DOI: 10.1126/science.1157643. A Cytosolic Iron Chaperone That Delivers Iron to Ferritin

A Cytosolic Iron Chaperone That Delivers Iron to Ferritin

10.1126/science.1157643

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Ferritins are the main iron storage proteins found in animals, plants, and bacteria. The capacity to store iron in ferritin is essential for life in mammals, but the mechanism by which cytosolic iron is delivered to ferritin is unknown. Human ferritins expressed in yeast contain little iron. Human poly (rC)–binding protein 1 (PCBP1) increased the amount of iron loaded into ferritin when expressed in yeast. PCBP1 bound to ferritin in vivo and bound iron and facilitated iron loading into ferritin in vitro. Depletion of PCBP1 in human cells inhibited ferritin iron loading and increased cytosolic iron pools. Thus, PCBP1 can function as a cytosolic iron chaperone in the delivery of iron to ferritin.

Bibliography

Shi, H., Bencze, K. Z., Stemmler, T. L., & Philpott, C. C. (2008). A Cytosolic Iron Chaperone That Delivers Iron to Ferritin. Science, 320(5880), 1207â1210.

Authors 4

Haifeng Shi (first)
Krisztina Z. Bencze (additional)
Timothy L. Stemmler (additional)
Caroline C. Philpott (additional)

References 20 Referenced 433

P. M. Harrison, P. Arosio, Biochim. Biophys. Acta1275, 161 (1996). (10.1016/0005-2728(96)00022-9) / Biochim. Biophys. Acta (1996)
E. C. Theil, M. Matzapetakis, X. Liu, J. Biol. Inorg. Chem.11, 803 (2006). (10.1007/s00775-006-0125-6) / J. Biol. Inorg. Chem. (2006)
F. Missirliset al., Genetics177, 89 (2007). (10.1534/genetics.107.075150) / Genetics (2007)
J. Kuchar, R. P. Hausinger, Chem. Rev.104, 509 (2004). (10.1021/cr020613p) / Chem. Rev. (2004)
10.1074/jbc.R000006200
10.1126/science.278.5339.853
10.1126/science.284.5415.805
10.1126/science.1098991
M. Pandolfo, J. Neural Transm. Suppl.70, 143 (2006). / J. Neural Transm. Suppl. (2006)
10.1016/0092-8674(94)90345-X
C. C. Philpott, O. Protchenko, Eukaryot. Cell7, 20 (2008). (10.1128/EC.00354-07) / Eukaryot. Cell (2008)
A. V. Makeyev, S. A. Liebhaber, RNA8, 265 (2002). (10.1017/S1355838202024627) / RNA (2002)
10.1038/nchembio807
10.1016/S1079-9796(03)00161-X
S. Epsztejn, O. Kakhlon, H. Glickstein, W. Breuer, I. Cabantchik, Anal. Biochem.248, 31 (1997). (10.1006/abio.1997.2126) / Anal. Biochem. (1997)
F. Bou-Abdallahet al., Biochemistry41, 11184 (2002). (10.1021/bi020215g) / Biochemistry (2002)
J. D. Cooket al., Biochemistry45, 7767 (2006). (10.1021/bi060424r) / Biochemistry (2006)
A. K. Wernimont, L. A. Yatsunyk, A. C. Rosenzweig, J. Biol. Chem.279, 12269 (2004). (10.1074/jbc.M311213200) / J. Biol. Chem. (2004)
L. Banciet al., Nat. Chem. Biol.2, 367 (2006). (10.1038/nchembio797) / Nat. Chem. Biol. (2006)
The authors thank S. Liebhaber for the PCBP1 plasmid protein samples and helpful discussions; H. Levin for helpful discussions and ferritin plasmids; P. Ponka for salicylaldehyde isonicotinoyl hydrazone; and T. Rouault for helpful discussions cell lines and the IRP2 antibody. These studies were supported by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases (H.S. and C.C.P.) and by NIH R01 DK068139 (K.Z.B. and T.L.S.).

Dates

Type	When
Created	17 years, 3 months ago (May 29, 2008, 4:53 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:33 a.m.)
Indexed	1 hour, 54 minutes ago (Aug. 30, 2025, 12:35 p.m.)
Issued	17 years, 3 months ago (May 30, 2008)
Published	17 years, 3 months ago (May 30, 2008)
Published Print	17 years, 3 months ago (May 30, 2008)

Funders 0

None

BibTeX

@article{Shi_2008, title={A Cytosolic Iron Chaperone That Delivers Iron to Ferritin}, volume={320}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1157643}, DOI={10.1126/science.1157643}, number={5880}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shi, Haifeng and Bencze, Krisztina Z. and Stemmler, Timothy L. and Philpott, Caroline C.}, year={2008}, month=may, pages={1207–1210} }

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