DOI: 10.1126/science.1151424. Single-Molecule Cut-and-Paste Surface Assembly

Single-Molecule Cut-and-Paste Surface Assembly

10.1126/science.1151424

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency.

Bibliography

Kufer, S. K., Puchner, E. M., Gumpp, H., Liedl, T., & Gaub, H. E. (2008). Single-Molecule Cut-and-Paste Surface Assembly. Science, 319(5863), 594â596.

Authors 5

S. K. Kufer (first)
E. M. Puchner (additional)
H. Gumpp (additional)
T. Liedl (additional)
H. E. Gaub (additional)

References 35 Referenced 238

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Helpful discussions with P. Hansma G. M. Whitesides J. Fernandez H. Heus P. Tinnefeld J. Morfill C. Albrecht and L. Whetton are gratefully acknowledged. Supported by the German Science Foundation and the Nanosystems Initiative Munich.

Dates

Type	When
Created	17 years, 6 months ago (Feb. 4, 2008, 7:49 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:19 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 2, 2025, 12:36 a.m.)
Issued	17 years, 6 months ago (Feb. 1, 2008)
Published	17 years, 6 months ago (Feb. 1, 2008)
Published Print	17 years, 6 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Kufer_2008, title={Single-Molecule Cut-and-Paste Surface Assembly}, volume={319}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1151424}, DOI={10.1126/science.1151424}, number={5863}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kufer, S. K. and Puchner, E. M. and Gumpp, H. and Liedl, T. and Gaub, H. E.}, year={2008}, month=feb, pages={594–596} }

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