DOI: 10.1126/science.1150057. Role of Intermolecular Forces in Defining Material Properties of Protein Nanofibrils

Role of Intermolecular Forces in Defining Material Properties of Protein Nanofibrils

10.1126/science.1150057

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Protein molecules have the ability to form a rich variety of natural and artificial structures and materials. We show that amyloid fibrils, ordered supramolecular nanostructures that are self-assembled from a wide range of polypeptide molecules, have rigidities varying over four orders of magnitude, and constitute a class of high-performance biomaterials. We elucidate the molecular origin of fibril material properties and show that the major contribution to their rigidity stems from a generic interbackbone hydrogen-bonding network that is modulated by variable side-chain interactions.

Bibliography

Knowles, T. P., Fitzpatrick, A. W., Meehan, S., Mott, H. R., Vendruscolo, M., Dobson, C. M., & Welland, M. E. (2007). Role of Intermolecular Forces in Defining Material Properties of Protein Nanofibrils. Science, 318(5858), 1900â1903.

Authors 7

Tuomas P. Knowles (first)
Anthony W. Fitzpatrick (additional)
Sarah Meehan (additional)
Helen R. Mott (additional)
Michele Vendruscolo (additional)
Christopher M. Dobson (additional)
Mark E. Welland (additional)

References 31 Referenced 707

D. M. Fowler, A. V. Koulov, W. E. Balch, J. W. Kelly, Trends Biochem. Sci.32, 217 (2007). (10.1016/j.tibs.2007.03.003) / Trends Biochem. Sci. (2007)
10.1146/annurev.biochem.75.101304.123901
M. Tanaka, S. R. Collins, B. H. Toyama, J. S. Weissman, Nature442, 585 (2006). (10.1038/nature04922) / Nature (2006)
D. M. Fowleret al., PLoS Biol.4, e6 (2005). (10.1371/journal.pbio.0040006) / PLoS Biol. (2005)
10.1038/nbt874
S. Zhang, T. Holmes, C. Lockshin, A. Rich, Proc. Natl. Acad. Sci. U.S.A.90, 3334 (1993). (10.1073/pnas.90.8.3334) / Proc. Natl. Acad. Sci. U.S.A. (1993)
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C. M. Dobson, Trends Biochem. Sci.24, 329 (1999). (10.1016/S0968-0004(99)01445-0) / Trends Biochem. Sci. (1999)
Materials and methods are available as supporting material on Science Online.
J. C. Wanget al., J. Mol. Biol.315, 601 (2002). (10.1006/jmbi.2001.5130) / J. Mol. Biol. (2002)
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P. T. Lansbury, H. A. Lashuel, Nature443, 774 (2006). (10.1038/nature05290) / Nature (2006)
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Single-letter abbreviations for the amino acid residues are as follows: A Ala; C Cys; D Asp; E Glu; F Phe; G Gly; H His; I Ile; K Lys; L Leu; M Met; N Asn; P Pro; Q Gln; R Arg; S Ser; T Thr; V Val; W Trp; and Y Tyr.
We acknowledge helpful discussions with E. Terentjev R. Hawkins S. Rogers and J. Smith as well as financial support from the Engineering and Physical Sciences Research Council and the Interdisciplinary Research Collaboration in Nanotechnology. We thank S. Shammas A. Brorsson and G. Devlin for preparing the Aβ and TTR fibrils and A. Tickler for synthesizing the TTR peptide. The work of C.M.D is supported in part by Programme Grants from the Wellcome Trust and the Leverhulme Trust.

Dates

Type	When
Created	17 years, 8 months ago (Dec. 20, 2007, 8:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:27 a.m.)
Indexed	52 minutes ago (Sept. 2, 2025, 11:31 p.m.)
Issued	17 years, 8 months ago (Dec. 21, 2007)
Published	17 years, 8 months ago (Dec. 21, 2007)
Published Print	17 years, 8 months ago (Dec. 21, 2007)

Funders 0

None

BibTeX

@article{Knowles_2007, title={Role of Intermolecular Forces in Defining Material Properties of Protein Nanofibrils}, volume={318}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1150057}, DOI={10.1126/science.1150057}, number={5858}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Knowles, Tuomas P. and Fitzpatrick, Anthony W. and Meehan, Sarah and Mott, Helen R. and Vendruscolo, Michele and Dobson, Christopher M. and Welland, Mark E.}, year={2007}, month=dec, pages={1900–1903} }

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      "unstructured": "We acknowledge helpful discussions with E. Terentjev R. Hawkins S. Rogers and J. Smith as well as financial support from the Engineering and Physical Sciences Research Council and the Interdisciplinary Research Collaboration in Nanotechnology. We thank S. Shammas A. Brorsson and G. Devlin for preparing the A\u03b2 and TTR fibrils and A. Tickler for synthesizing the TTR peptide. The work of C.M.D is supported in part by Programme Grants from the Wellcome Trust and the Leverhulme Trust."
    }
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