DOI: 10.1126/science.1145980. Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex

Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex

10.1126/science.1145980

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The nuclear pore complex regulates cargo transport between the cytoplasm and the nucleus. We set out to correlate the governing biochemical interactions to the nanoscopic responses of the phenylalanineglycine (FG)–rich nucleoporin domains, which are involved in attenuating or promoting cargo translocation. We found that binding interactions with the transport receptor karyopherin-β1 caused the FG domains of the human nucleoporin Nup153 to collapse into compact molecular conformations. This effect was reversed by the action of Ran guanosine triphosphate, which returned the FG domains into a polymer brush-like, entropic barrier conformation. Similar effects were observed in Xenopus oocyte nuclei in situ. Thus, the reversible collapse of the FG domains may play an important role in regulating nucleocytoplasmic transport.

Bibliography

Lim, R. Y. H., Fahrenkrog, B., KoÌser, J., Schwarz-Herion, K., Deng, J., & Aebi, U. (2007). Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex. Science, 318(5850), 640â643.

Authors 6

Roderick Y. H. Lim (first)
Birthe Fahrenkrog (additional)
Joachim Köser (additional)
Kyrill Schwarz-Herion (additional)
Jie Deng (additional)
Ueli Aebi (additional)

References 30 Referenced 274

10.1016/S0092-8674(03)00082-5
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We thank K. S. Ullman for providing antibodies to C2-Nup153. Supported by the National Center of Competence in Research in Nanoscale Science the Swiss National Science Foundation the M. E. Müller Foundation of Switzerland and the Canton Basel-Stadt.

Dates

Type	When
Created	17 years, 10 months ago (Oct. 4, 2007, 8:59 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 4:19 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 12, 2025, 5:35 p.m.)
Issued	17 years, 10 months ago (Oct. 26, 2007)
Published	17 years, 10 months ago (Oct. 26, 2007)
Published Print	17 years, 10 months ago (Oct. 26, 2007)

Funders 0

None

BibTeX

@article{Lim_2007, title={Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex}, volume={318}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1145980}, DOI={10.1126/science.1145980}, number={5850}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lim, Roderick Y. H. and Fahrenkrog, Birthe and Köser, Joachim and Schwarz-Herion, Kyrill and Deng, Jie and Aebi, Ueli}, year={2007}, month=oct, pages={640–643} }

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