DOI: 10.1126/science.1145977. Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7

Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7

10.1126/science.1145977

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr 1 -Ser 2 -Pro 3 -Thr 4 -Ser 5 -Pro 6 -Ser 7 . Differential phosphorylation of serine-2 and serine-5 at the 5′ and 3′ regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.

Bibliography

Chapman, R. D., Heidemann, M., Albert, T. K., Mailhammer, R., Flatley, A., Meisterernst, M., Kremmer, E., & Eick, D. (2007). Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7. Science, 318(5857), 1780â1782.

Authors 8

Rob D. Chapman (first)
Martin Heidemann (additional)
Thomas K. Albert (additional)
Reinhard Mailhammer (additional)
Andrew Flatley (additional)
Michael Meisterernst (additional)
Elisabeth Kremmer (additional)
Dirk Eick (additional)

References 16 Referenced 263

10.1101/gad.1318105
10.1101/gad.1477006
10.1046/j.1432-1033.2003.03794.x
E. Barron-Casella, J. L. Corden, J. Mol. Evol.35, 405 (1992). / J. Mol. Evol. (1992)
M. S. Bartolomei, N. F. Halden, C. R. Cullen, J. L. Corden, Mol. Cell. Biol.8, 330 (1988). / Mol. Cell. Biol. (1988)
10.1128/MCB.25.17.7665-7674.2005
10.1093/genetics/140.4.1223
10.1074/jbc.M102170200
10.1038/nsmb1155
10.1074/jbc.M001883200
10.1128/EC.3.3.735-740.2004
10.1074/jbc.C500423200
10.1038/sj.emboj.7600876
10.1126/science.1145989
10.1038/nsb0903-679
Supported by grants from the Deutsche Forschungsgemeinschaft (SFB/TR5 and SFB684) Bundesministerium für Bildung und Forschung (QuantPRO 0313860) Boehringer Ingelheim Fonds and Fonds der Chemischen Industrie. We thank A. Meinhart and P. Cramer for critical comments on this manuscript.

Dates

Type	When
Created	17 years, 8 months ago (Dec. 13, 2007, 4:43 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 2:50 a.m.)
Indexed	1 week ago (Aug. 20, 2025, 8:25 a.m.)
Issued	17 years, 8 months ago (Dec. 14, 2007)
Published	17 years, 8 months ago (Dec. 14, 2007)
Published Print	17 years, 8 months ago (Dec. 14, 2007)

Funders 0

None

BibTeX

@article{Chapman_2007, title={Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7}, volume={318}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1145977}, DOI={10.1126/science.1145977}, number={5857}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chapman, Rob D. and Heidemann, Martin and Albert, Thomas K. and Mailhammer, Reinhard and Flatley, Andrew and Meisterernst, Michael and Kremmer, Elisabeth and Eick, Dirk}, year={2007}, month=dec, pages={1780–1782} }

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