DOI: 10.1126/science.1143837. Genomic Minimalism in the Early Diverging Intestinal Parasite <i>Giardia lamblia</i>

Genomic Minimalism in the Early Diverging Intestinal Parasite Giardia lamblia

10.1126/science.1143837

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The genome of the eukaryotic protist Giardia lamblia, an important human intestinal parasite, is compact in structure and content, contains few introns or mitochondrial relics, and has simplified machinery for DNA replication, transcription, RNA processing, and most metabolic pathways. Protein kinases comprise the single largest protein class and reflect Giardia's requirement for a complex signal transduction network for coordinating differentiation. Lateral gene transfer from bacterial and archaeal donors has shaped Giardia's genome, and previously unknown gene families, for example, cysteine-rich structural proteins, have been discovered. Unexpectedly, the genome shows little evidence of heterozygosity, supporting recent speculations that this organism is sexual. This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite.

Bibliography

Morrison, H. G., McArthur, A. G., Gillin, F. D., Aley, S. B., Adam, R. D., Olsen, G. J., Best, A. A., Cande, W. Z., Chen, F., Cipriano, M. J., Davids, B. J., Dawson, S. C., Elmendorf, H. G., Hehl, A. B., Holder, M. E., Huse, S. M., Kim, U. U., Lasek-Nesselquist, E., Manning, G., â¦ Sogin, M. L. (2007). Genomic Minimalism in the Early Diverging Intestinal Parasite Giardia lamblia. Science, 317(5846), 1921â1926.

Authors 29

Hilary G. Morrison (first)
Andrew G. McArthur (additional)
Frances D. Gillin (additional)
Stephen B. Aley (additional)
Rodney D. Adam (additional)
Gary J. Olsen (additional)
Aaron A. Best (additional)
W. Zacheus Cande (additional)
Feng Chen (additional)
Michael J. Cipriano (additional)
Barbara J. Davids (additional)
Scott C. Dawson (additional)
Heidi G. Elmendorf (additional)
Adrian B. Hehl (additional)
Michael E. Holder (additional)
Susan M. Huse (additional)
Ulandt U. Kim (additional)
Erica Lasek-Nesselquist (additional)
Gerard Manning (additional)
Anuranjini Nigam (additional)
Julie E. J. Nixon (additional)
Daniel Palm (additional)
Nora E. Passamaneck (additional)
Anjali Prabhu (additional)
Claudia I. Reich (additional)
David S. Reiner (additional)
John Samuelson (additional)
Staffan G. Svard (additional)
Mitchell L. Sogin (additional)

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We acknowledge J. D. Silberman S. (Pacocha) Preheim S. Birkeland M. Shapiro V. Seshadri and J. Woo for laboratory assistance and M. Pop S. Salzburg G. Hinkle and R. Campbell for assistance with informatics and helpful discussions. We are grateful to The Institute for Genomic Research the Sanger Centre and the Joint Genome Institute for use of sequence data. The NIH/National Institute of Allergy and Infectious Diseases (grants AI43273 to M.L.S. and AI42488/AI51687 to F.D.G.) the G. Unger Vetlesen Foundation the Ellison Medical Foundation and LI-COR Biotechnology supported this work. This whole-genome shotgun project has been deposited at the DNA Databank of Japan/European Molecular Biology Laboratory/GenBank under the project accession AACB00000000. The version described in this paper is the second version AACB02000000.

Dates

Type	When
Created	17 years, 11 months ago (Sept. 27, 2007, 4:44 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:31 a.m.)
Indexed	5 days, 2 hours ago (Sept. 2, 2025, 6:39 a.m.)
Issued	17 years, 11 months ago (Sept. 28, 2007)
Published	17 years, 11 months ago (Sept. 28, 2007)
Published Print	17 years, 11 months ago (Sept. 28, 2007)

Funders 0

None

BibTeX

@article{Morrison_2007, title={Genomic Minimalism in the Early Diverging Intestinal Parasite Giardia lamblia}, volume={317}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1143837}, DOI={10.1126/science.1143837}, number={5846}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Morrison, Hilary G. and McArthur, Andrew G. and Gillin, Frances D. and Aley, Stephen B. and Adam, Rodney D. and Olsen, Gary J. and Best, Aaron A. and Cande, W. Zacheus and Chen, Feng and Cipriano, Michael J. and Davids, Barbara J. and Dawson, Scott C. and Elmendorf, Heidi G. and Hehl, Adrian B. and Holder, Michael E. and Huse, Susan M. and Kim, Ulandt U. and Lasek-Nesselquist, Erica and Manning, Gerard and Nigam, Anuranjini and Nixon, Julie E. J. and Palm, Daniel and Passamaneck, Nora E. and Prabhu, Anjali and Reich, Claudia I. and Reiner, David S. and Samuelson, John and Svard, Staffan G. and Sogin, Mitchell L.}, year={2007}, month=sep, pages={1921–1926} }

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  "abstract": "<jats:p>The genome of the eukaryotic protist Giardia lamblia, an important human intestinal parasite, is compact in structure and content, contains few introns or mitochondrial relics, and has simplified machinery for DNA replication, transcription, RNA processing, and most metabolic pathways. Protein kinases comprise the single largest protein class and reflect Giardia's requirement for a complex signal transduction network for coordinating differentiation. Lateral gene transfer from bacterial and archaeal donors has shaped Giardia's genome, and previously unknown gene families, for example, cysteine-rich structural proteins, have been discovered. Unexpectedly, the genome shows little evidence of heterozygosity, supporting recent speculations that this organism is sexual. This genome sequence will not only be valuable for investigating the evolution of eukaryotes, but will also be applied to the search for new therapeutics for this parasite.</jats:p>",
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