CPD Damage Recognition by Transcribing RNA Polymerase II
10.1126/science.1135400
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Cells use transcription-coupled repair (TCR) to efficiently eliminate DNA lesions such as ultraviolet light–induced cyclobutane pyrimidine dimers (CPDs). Here we present the structure-based mechanism for the first step in eukaryotic TCR, CPD-induced stalling of RNA polymerase (Pol) II. A CPD in the transcribed strand slowly passes a translocation barrier and enters the polymerase active site. The CPD 5′-thymine then directs uridine misincorporation into messenger RNA, which blocks translocation. Artificial replacement of the uridine by adenosine enables CPD bypass; thus, Pol II stalling requires CPD-directed misincorporation. In the stalled complex, the lesion is inaccessible, and the polymerase conformation is unchanged. This is consistent with nonallosteric recruitment of repair factors and excision of a lesion-containing DNA fragment in the presence of Pol II.

Bibliography

Brueckner, F., Hennecke, U., Carell, T., & Cramer, P. (2007). CPD Damage Recognition by Transcribing RNA Polymerase II. Science, 315(5813), 859–862.

Authors 4
  1. Florian Brueckner (first)
  2. Ulrich Hennecke (additional)
  3. Thomas Carell (additional)
  4. Patrick Cramer (additional)
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Dates
Type When
Created 18 years, 6 months ago (Feb. 8, 2007, 4:33 p.m.)
Deposited 1 year, 7 months ago (Jan. 10, 2024, 4:40 a.m.)
Indexed 7 hours, 22 minutes ago (Aug. 27, 2025, 11:39 a.m.)
Issued 18 years, 6 months ago (Feb. 9, 2007)
Published 18 years, 6 months ago (Feb. 9, 2007)
Published Print 18 years, 6 months ago (Feb. 9, 2007)
Funders 0

None

@article{Brueckner_2007, title={CPD Damage Recognition by Transcribing RNA Polymerase II}, volume={315}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1135400}, DOI={10.1126/science.1135400}, number={5813}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Brueckner, Florian and Hennecke, Ulrich and Carell, Thomas and Cramer, Patrick}, year={2007}, month=feb, pages={859–862} }