DOI: 10.1126/science.1133427. The Consensus Coding Sequences of Human Breast and Colorectal Cancers

The Consensus Coding Sequences of Human Breast and Colorectal Cancers

10.1126/science.1133427

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The elucidation of the human genome sequence has made it possible to identify genetic alterations in cancers in unprecedented detail. To begin a systematic analysis of such alterations, we determined the sequence of well-annotated human protein-coding genes in two common tumor types. Analysis of 13,023 genes in 11 breast and 11 colorectal cancers revealed that individual tumors accumulate an average of ∼90 mutant genes but that only a subset of these contribute to the neoplastic process. Using stringent criteria to delineate this subset, we identified 189 genes (average of 11 per tumor) that were mutated at significant frequency. The vast majority of these genes were not known to be genetically altered in tumors and are predicted to affect a wide range of cellular functions, including transcription, adhesion, and invasion. These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention, and open fertile avenues for basic research in tumor biology.

Bibliography

SjoÌblom, T., Jones, S., Wood, L. D., Parsons, D. W., Lin, J., Barber, T. D., Mandelker, D., Leary, R. J., Ptak, J., Silliman, N., Szabo, S., Buckhaults, P., Farrell, C., Meeh, P., Markowitz, S. D., Willis, J., Dawson, D., Willson, J. K. V., Gazdar, A. F., â¦ Velculescu, V. E. (2006). The Consensus Coding Sequences of Human Breast and Colorectal Cancers. Science, 314(5797), 268â274.

Authors 29

Tobias Sjöblom (first)
Siân Jones (additional)
Laura D. Wood (additional)
D. Williams Parsons (additional)
Jimmy Lin (additional)
Thomas D. Barber (additional)
Diana Mandelker (additional)
Rebecca J. Leary (additional)
Janine Ptak (additional)
Natalie Silliman (additional)
Steve Szabo (additional)
Phillip Buckhaults (additional)
Christopher Farrell (additional)
Paul Meeh (additional)
Sanford D. Markowitz (additional)
Joseph Willis (additional)
Dawn Dawson (additional)
James K. V. Willson (additional)
Adi F. Gazdar (additional)
James Hartigan (additional)
Leo Wu (additional)
Changsheng Liu (additional)
Giovanni Parmigiani (additional)
Ben Ho Park (additional)
Kurtis E. Bachman (additional)
Nickolas Papadopoulos (additional)
Bert Vogelstein (additional)
Kenneth W. Kinzler (additional)
Victor E. Velculescu (additional)

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We thank J. Lutterbaugh E. Lawrence and L. Beard for assistance with cell culture and DNA preparation; E. Suh D. Smith K. Makowski and the Agencourt sequencing team for assistance with automated sequencing; S. Kern for helpful comments on the manuscript; and R. J. Vogelstein and J. T. Vogelstein for assistance with statistical analyses. Supported by the Virginia and D. K. Ludwig Fund for Cancer Research; NIH grants CA 121113 CA 43460 CA 57345 CA 62924 GM 07309 RR 017698 P30-CA43703 and CA109274; NCI Division of Cancer Prevention contract HHSN261200433002C; Department of Defense grant DAMD17-03-1-0241; and the Pew Charitable Trusts the Palmetto Health Foundation the Maryland Cigarette Restitution Fund the State of Ohio Biomedical Research and Technology Transfer Commission the Clayton Fund the Blaustein Foundation the National Colorectal Cancer Research Alliance the Avon Foundation the Flight Attendant Medical Research Institute and the V Foundation for Cancer Research.

Dates

Type	When
Created	18 years, 11 months ago (Sept. 7, 2006, 8:53 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 4:57 a.m.)
Indexed	14 hours, 21 minutes ago (Sept. 6, 2025, 3:36 p.m.)
Issued	18 years, 10 months ago (Oct. 13, 2006)
Published	18 years, 10 months ago (Oct. 13, 2006)
Published Print	18 years, 10 months ago (Oct. 13, 2006)

Funders 0

None

BibTeX

@article{Sjo_blom_2006, title={The Consensus Coding Sequences of Human Breast and Colorectal Cancers}, volume={314}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1133427}, DOI={10.1126/science.1133427}, number={5797}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sjöblom, Tobias and Jones, Siân and Wood, Laura D. and Parsons, D. Williams and Lin, Jimmy and Barber, Thomas D. and Mandelker, Diana and Leary, Rebecca J. and Ptak, Janine and Silliman, Natalie and Szabo, Steve and Buckhaults, Phillip and Farrell, Christopher and Meeh, Paul and Markowitz, Sanford D. and Willis, Joseph and Dawson, Dawn and Willson, James K. V. and Gazdar, Adi F. and Hartigan, James and Wu, Leo and Liu, Changsheng and Parmigiani, Giovanni and Park, Ben Ho and Bachman, Kurtis E. and Papadopoulos, Nickolas and Vogelstein, Bert and Kinzler, Kenneth W. and Velculescu, Victor E.}, year={2006}, month=oct, pages={268–274} }

JSON

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