DOI: 10.1126/science.1133281. Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA

Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA

10.1126/science.1133281

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis -acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo–electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.

Bibliography

Pfingsten, J. S., Costantino, D. A., & Kieft, J. S. (2006). Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA. Science, 314(5804), 1450â1454.

Authors 3

Jennifer S. Pfingsten (first)
David A. Costantino (additional)
Jeffrey S. Kieft (additional)

References 28 Referenced 135

J. W. B. Hershey, W. C. Merrick, in Translational Control of Gene Expression, N. Sonenberg, J. W. B. Hershey, M. B. Mathews, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2000), pp. 33–88. / Translational Control of Gene Expression (2000)
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We acknowledge the staff at beamline ALS 8.2.1 for assistance R. Zhao for managing the University of Colorado (UC) at Denver and Health Sciences Center x-ray facility D. Farrell for computer administration and M. Churchill R. Batey and A. Ferré-D'Amaré for useful discussions and advice. We especially thank C. Spahn for supplying various cryo-EM density files and R. Batey for the iridium (III) hexammine. R. Batey M. Churchill D. Bentley L. Krushel and R. Zhao provided a critical reading of this manuscript. This work was supported by a grant from NIH and funding from the UC Cancer Center in support of the x-ray facility. Structure factors and coordinates have been deposited in the Protein Data Bank under accession code 2IL9.

Dates

Type	When
Created	18 years, 9 months ago (Nov. 23, 2006, 8:48 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 4:15 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	18 years, 8 months ago (Dec. 1, 2006)
Published	18 years, 8 months ago (Dec. 1, 2006)
Published Print	18 years, 8 months ago (Dec. 1, 2006)

Funders 0

None

BibTeX

@article{Pfingsten_2006, title={Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA}, volume={314}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1133281}, DOI={10.1126/science.1133281}, number={5804}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Pfingsten, Jennifer S. and Costantino, David A. and Kieft, Jeffrey S.}, year={2006}, month=dec, pages={1450–1454} }

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