DOI: 10.1126/science.1132998. RIG-I-Mediated Antiviral Responses to Single-Stranded RNA Bearing 5'-Phosphates

RIG-I-Mediated Antiviral Responses to Single-Stranded RNA Bearing 5'-Phosphates

10.1126/science.1132998

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Double-stranded RNA (dsRNA) produced during viral replication is believed to be the critical trigger for activation of antiviral immunity mediated by the RNA helicase enzymes retinoic acid–inducible gene I (RIG-I) and melanoma differentiation–associated gene 5 (MDA5). We showed that influenza A virus infection does not generate dsRNA and that RIG-I is activated by viral genomic single-stranded RNA (ssRNA) bearing 5′-phosphates. This is blocked by the influenza protein nonstructured protein 1 (NS1), which is found in a complex with RIG-I in infected cells. These results identify RIG-I as a ssRNA sensor and potential target of viral immune evasion and suggest that its ability to sense 5'-phosphorylated RNA evolved in the innate immune system as a means of discriminating between self and nonself.

Bibliography

Pichlmair, A., Schulz, O., Tan, C. P., NaÌslund, T. I., LiljestroÌm, P., Weber, F., & Reis e Sousa, C. (2006). RIG-I-Mediated Antiviral Responses to Single-Stranded RNA Bearing 5â-Phosphates. Science, 314(5801), 997â1001.

Authors 7

Andreas Pichlmair (first)
Oliver Schulz (additional)
Choon Ping Tan (additional)
Tanja I. Näslund (additional)
Peter Liljeström (additional)
Friedemann Weber (additional)
Caetano Reis e Sousa (additional)

References 25 Referenced 1,832

10.1146/annurev.immunol.23.021704.115843
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This work was funded by Cancer Research UK. We thank I. Kerr for SFV and EMCV T. Muster for ΔNS1 J. Skehel for purified influenza S. Diebold for flu vRNA isolation and GFP RNA synthesis A. Bergthaler for VSV vRNA J. Yewdell for the antibody to NS1 C. Basler for FLAG–RIG-I and A. Garcia-Sastre for NS1 constructs. We are grateful to members of the Immunobiology Laboratory for support.

Dates

Type	When
Created	18 years, 10 months ago (Oct. 13, 2006, 6:40 a.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 4:28 a.m.)
Indexed	1 hour, 56 minutes ago (Aug. 24, 2025, 4:50 a.m.)
Issued	18 years, 9 months ago (Nov. 10, 2006)
Published	18 years, 9 months ago (Nov. 10, 2006)
Published Print	18 years, 9 months ago (Nov. 10, 2006)

Funders 0

None

BibTeX

@article{Pichlmair_2006, title={RIG-I-Mediated Antiviral Responses to Single-Stranded RNA Bearing 5’-Phosphates}, volume={314}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1132998}, DOI={10.1126/science.1132998}, number={5801}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Pichlmair, Andreas and Schulz, Oliver and Tan, Choon Ping and Näslund, Tanja I. and Liljeström, Peter and Weber, Friedemann and Reis e Sousa, Caetano}, year={2006}, month=nov, pages={997–1001} }

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