DOI: 10.1126/science.1122689. Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs

Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs

10.1126/science.1122689

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.

Bibliography

Giraldez, A. J., Mishima, Y., Rihel, J., Grocock, R. J., Van Dongen, S., Inoue, K., Enright, A. J., & Schier, A. F. (2006). Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science, 312(5770), 75â79.

Authors 8

Antonio J. Giraldez (first)
Yuichiro Mishima (additional)
Jason Rihel (additional)
Russell J. Grocock (additional)
Stijn Van Dongen (additional)
Kunio Inoue (additional)
Anton J. Enright (additional)
Alexander F. Schier (additional)

References 40 Referenced 1,331

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We thank C. Antonio I. Baptista V. Greco H. Knaut H. Lopez-Schier S. Mango T. Schell and W. Talbot for providing helpful comments on the manuscript. A.J.G. was supported by European Molecular Biology Organisation and is currently supported by a Human Frontier Science Program fellowship. A.F.S. was an Irma T. Hirschl Trust Career Scientist and an Established Investigator of the American Heart Association. This work was also supported by grants from the NIH (A.F.S.). Array data has been deposited in Gene Expression Omnibus (GEO) database under accession number GSE4201.

Dates

Type	When
Created	19 years, 6 months ago (Feb. 16, 2006, 8:44 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:37 p.m.)
Indexed	41 minutes ago (Sept. 4, 2025, 5:50 a.m.)
Issued	19 years, 4 months ago (April 7, 2006)
Published	19 years, 4 months ago (April 7, 2006)
Published Print	19 years, 4 months ago (April 7, 2006)

Funders 0

None

BibTeX

@article{Giraldez_2006, title={Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs}, volume={312}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1122689}, DOI={10.1126/science.1122689}, number={5770}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Giraldez, Antonio J. and Mishima, Yuichiro and Rihel, Jason and Grocock, Russell J. and Van Dongen, Stijn and Inoue, Kunio and Enright, Anton J. and Schier, Alexander F.}, year={2006}, month=apr, pages={75–79} }

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