DOI: 10.1126/science.1117551. Photosynthetic O <sub>2</sub> Formation Tracked by Time-Resolved X-ray Experiments

Photosynthetic O 2 Formation Tracked by Time-Resolved X-ray Experiments

10.1126/science.1117551

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Plants and cyanobacteria produce atmospheric dioxygen from water, powered by sunlight and catalyzed by a manganese complex in photosystem II. A classic S-cycle model for oxygen evolution involves five states, but only four have been identified. The missing S 4 state is particularly important because it is directly involved in dioxygen formation. Now progress comes from an x-ray technique that can monitor redox and structural changes in metal centers in real time with 10-microsecond resolution. We show that in the O 2 -formation step, an intermediate is formed—the enigmatic S 4 state. Its creation is identified with a deprotonation process rather than the expected electron-transfer mechanism. Subsequent electron transfer would give an additional S 4 ′ state, thus extending the fundamental S-state cycle of dioxygen formation.

Bibliography

Haumann, M., Liebisch, P., MuÌller, C., Barra, M., Grabolle, M., & Dau, H. (2005). Photosynthetic O 2 Formation Tracked by Time-Resolved X-ray Experiments. Science, 310(5750), 1019â1021.

Authors 6

M. Haumann (first)
P. Liebisch (additional)
C. Müller (additional)
M. Barra (additional)
M. Grabolle (additional)
H. Dau (additional)

References 31 Referenced 426

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The x-ray experiments were carried out at the experimental station ID26 of the European Synchrotron Radiation Facility (ESRF) (Grenoble France). We thank T. Neisius S. Eeckhout and P. Glatzel (all of ESRF) who contributed significantly to the preparation of the x-ray experiment and P. Loja (of our research group) for her important contributions to XAS data collection. Financial support by the Deutsche Forschungsgemeinschaft (DFG) (Collaborative Research Center SFB 498 projects C6 and C8) and the Bundesministerium für Bildung und Forschung (BMBF) (grant 05KS1KEA/6) is gratefully acknowledged.

Dates

Type	When
Created	19 years, 9 months ago (Nov. 10, 2005, 5:33 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:25 p.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 9:28 a.m.)
Issued	19 years, 9 months ago (Nov. 11, 2005)
Published	19 years, 9 months ago (Nov. 11, 2005)
Published Print	19 years, 9 months ago (Nov. 11, 2005)

Funders 0

None

BibTeX

@article{Haumann_2005, title={Photosynthetic O 2 Formation Tracked by Time-Resolved X-ray Experiments}, volume={310}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1117551}, DOI={10.1126/science.1117551}, number={5750}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Haumann, M. and Liebisch, P. and Müller, C. and Barra, M. and Grabolle, M. and Dau, H.}, year={2005}, month=nov, pages={1019–1021} }

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