DOI: 10.1126/science.1114955. RNA Polymerase II Is Required for RNAi-Dependent Heterochromatin Assembly

RNA Polymerase II Is Required for RNAi-Dependent Heterochromatin Assembly

10.1126/science.1114955

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In Schizosaccharomyces pombe , the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.

Bibliography

Kato, H., Goto, D. B., Martienssen, R. A., Urano, T., Furukawa, K., & Murakami, Y. (2005). RNA Polymerase II Is Required for RNAi-Dependent Heterochromatin Assembly. Science, 309(5733), 467â469.

Authors 6

Hiroaki Kato (first)
Derek B. Goto (additional)
Robert A. Martienssen (additional)
Takeshi Urano (additional)
Koichi Furukawa (additional)
Yota Murakami (additional)

References 21 Referenced 234

10.1126/science.1093686
10.1016/j.cell.2004.11.034
10.1126/science.1074973
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Materials and methods are available as supporting material on Science Online.
10.1126/science.1060118
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We thank R. C. Allshire S. I. S. Grewal D. Q. Ding and Y. Hiraoka for their gifts of strains and plasmids and members of the Murakami laboratory and K. Tanaka for their support and discussions. D.B.G. is a U.S. Department of Energy–Energy Biosciences Research Fellow of the Life Sciences Research Foundation. This work is supported in part by a grant from NIH (R01-GM067014) to R.A.M. and by a Grant-in-aid for Scientific Research (B) to Y.M. from the Japan Society for the Promotion of Science.

Dates

Type	When
Created	20 years, 2 months ago (June 9, 2005, 8:44 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:40 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 10:07 a.m.)
Issued	20 years, 1 month ago (July 15, 2005)
Published	20 years, 1 month ago (July 15, 2005)
Published Print	20 years, 1 month ago (July 15, 2005)

Funders 0

None

BibTeX

@article{Kato_2005, title={RNA Polymerase II Is Required for RNAi-Dependent Heterochromatin Assembly}, volume={309}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1114955}, DOI={10.1126/science.1114955}, number={5733}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kato, Hiroaki and Goto, Derek B. and Martienssen, Robert A. and Urano, Takeshi and Furukawa, Koichi and Murakami, Yota}, year={2005}, month=jul, pages={467–469} }

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