DOI: 10.1126/science.1104808. Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography

Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography

10.1126/science.1104808

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

Bibliography

Beck, M., FoÌrster, F., Ecke, M., Plitzko, J. M., Melchior, F., Gerisch, G., Baumeister, W., & Medalia, O. (2004). Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography. Science, 306(5700), 1387â1390.

Authors 8

Martin Beck (first)
Friedrich Förster (additional)
Mary Ecke (additional)
Jürgen M. Plitzko (additional)
Frauke Melchior (additional)
Günther Gerisch (additional)
Wolfgang Baumeister (additional)
Ohad Medalia (additional)

References 18 Referenced 435

10.1016/S1534-5807(03)00162-X
10.1016/S0022-2836(03)00266-3
10.1016/S0959-440X(02)00378-0
10.1126/science.1076184
10.1083/jcb.123.6.1649
Because the NPCs of spread Xenopus nuclear envelopes have a preferred orientation ( 17 ) an isotropically resolved structure cannot be obtained from such samples (although transport active) owing to the missing cone problem ( 11 ).
10.1006/jmbi.1996.0206
10.1016/j.jsb.2003.11.010
10.1016/S1097-2765(00)80023-4
10.1083/jcb.122.1.1
K. Grunewald, O. Medalia, A. Gross, A. C. Steven, W. Baumeister, Biophys. Chem.100, 577 (2003). / Biophys. Chem. (2003)
10.1083/jcb.148.4.635
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10.1126/science.273.5282.1729
10.1038/nrm1230
10.1073/pnas.0403675101
10.1093/embo-reports/kvf171
We thank R. Hegerl for help with the image processing and A. Leis V. Lucic and P. Zwickl for critical reading of the manuscript.

Dates

Type	When
Created	20 years, 9 months ago (Oct. 28, 2004, 8:33 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 11:44 p.m.)
Indexed	3 weeks, 1 day ago (July 30, 2025, 10:38 a.m.)
Issued	20 years, 9 months ago (Nov. 19, 2004)
Published	20 years, 9 months ago (Nov. 19, 2004)
Published Print	20 years, 9 months ago (Nov. 19, 2004)

Funders 0

None

BibTeX

@article{Beck_2004, title={Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography}, volume={306}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1104808}, DOI={10.1126/science.1104808}, number={5700}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Beck, Martin and Förster, Friedrich and Ecke, Mary and Plitzko, Jürgen M. and Melchior, Frauke and Gerisch, Günther and Baumeister, Wolfgang and Medalia, Ohad}, year={2004}, month=nov, pages={1387–1390} }

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