DOI: 10.1126/science.1101156. The Genome of the Diatom <i>Thalassiosira Pseudonana</i> : Ecology, Evolution, and Metabolism

The Genome of the Diatom Thalassiosira Pseudonana : Ecology, Evolution, and Metabolism

10.1126/science.1101156

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for ∼20% of global carbon fixation. We report the 34 million–base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand–base pair plastid and 44 thousand–base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.

Authors 45

E. Virginia Armbrust (first)
John A. Berges (additional)
Chris Bowler (additional)
Beverley R. Green (additional)
Diego Martinez (additional)
Nicholas H. Putnam (additional)
Shiguo Zhou (additional)
Andrew E. Allen (additional)
Kirk E. Apt (additional)
Michael Bechner (additional)
Mark A. Brzezinski (additional)
Balbir K. Chaal (additional)
Anthony Chiovitti (additional)
Aubrey K. Davis (additional)
Mark S. Demarest (additional)
J. Chris Detter (additional)
Tijana Glavina (additional)
David Goodstein (additional)
Masood Z. Hadi (additional)
Uffe Hellsten (additional)
Mark Hildebrand (additional)
Bethany D. Jenkins (additional)
Jerzy Jurka (additional)
Vladimir V. Kapitonov (additional)
Nils Kröger (additional)
Winnie W. Y. Lau (additional)
Todd W. Lane (additional)
Frank W. Larimer (additional)
J. Casey Lippmeier (additional)
Susan Lucas (additional)
Mónica Medina (additional)
Anton Montsant (additional)
Miroslav Obornik (additional)
Micaela Schnitzler Parker (additional)
Brian Palenik (additional)
Gregory J. Pazour (additional)
Paul M. Richardson (additional)
Tatiana A. Rynearson (additional)
Mak A. Saito (additional)
David C. Schwartz (additional)
Kimberlee Thamatrakoln (additional)
Klaus Valentin (additional)
Assaf Vardi (additional)
Frances P. Wilkerson (additional)
Daniel S. Rokhsar (additional)

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This work was performed under the auspices of the U.S. Department of Energy's (DOE) Office of Science Biological and Environmental Research Program and by the University of California Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48 Lawrence Berkeley National Laboratory under contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36.; and DOE (DE-FG03-02ER63471 to E.V.A.) European Union Margens (QLRT-2001-01226 to C.B.) the CNRS Atip program (2JE144 to C.B.) and the U.S. Environmental Protection Agency (R827107-01-0 Basic to B.P. M.H.).

Dates

Type	When
Created	20 years, 10 months ago (Sept. 30, 2004, 5:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:20 p.m.)
Indexed	2 days, 6 hours ago (Aug. 20, 2025, 9:06 a.m.)
Issued	20 years, 10 months ago (Oct. 1, 2004)
Published	20 years, 10 months ago (Oct. 1, 2004)
Published Print	20 years, 10 months ago (Oct. 1, 2004)

Funders 0

None

BibTeX

@article{Armbrust_2004, title={The Genome of the Diatom Thalassiosira Pseudonana  : Ecology, Evolution, and Metabolism}, volume={306}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1101156}, DOI={10.1126/science.1101156}, number={5693}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Armbrust, E. Virginia and Berges, John A. and Bowler, Chris and Green, Beverley R. and Martinez, Diego and Putnam, Nicholas H. and Zhou, Shiguo and Allen, Andrew E. and Apt, Kirk E. and Bechner, Michael and Brzezinski, Mark A. and Chaal, Balbir K. and Chiovitti, Anthony and Davis, Aubrey K. and Demarest, Mark S. and Detter, J. Chris and Glavina, Tijana and Goodstein, David and Hadi, Masood Z. and Hellsten, Uffe and Hildebrand, Mark and Jenkins, Bethany D. and Jurka, Jerzy and Kapitonov, Vladimir V. and Kröger, Nils and Lau, Winnie W. Y. and Lane, Todd W. and Larimer, Frank W. and Lippmeier, J. Casey and Lucas, Susan and Medina, Mónica and Montsant, Anton and Obornik, Miroslav and Parker, Micaela Schnitzler and Palenik, Brian and Pazour, Gregory J. and Richardson, Paul M. and Rynearson, Tatiana A. and Saito, Mak A. and Schwartz, David C. and Thamatrakoln, Kimberlee and Valentin, Klaus and Vardi, Assaf and Wilkerson, Frances P. and Rokhsar, Daniel S.}, year={2004}, month=oct, pages={79–86} }

JSON

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  "abstract": "<jats:p>\n            Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for \u223c20% of global carbon fixation. We report the 34 million\u2013base pair draft nuclear genome of the marine diatom\n            <jats:italic>Thalassiosira pseudonana</jats:italic>\n            and its 129 thousand\u2013base pair plastid and 44 thousand\u2013base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.\n          </jats:p>",
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  "title": "The Genome of the Diatom\n            <i>Thalassiosira Pseudonana</i>\n            : Ecology, Evolution, and Metabolism",
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          "name": "Laboratory of Molecular Plant Biology, Stazione Zoologica, Villa Comunale, I 80121 Naples, Italy. Organismes Photosynthe\u0301tiques et Environnement, De\u0301partement de Biologie, 75230 Paris, France."
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        {
          "name": "Ecole Normale Supe\u0301rieure, 75230 Paris, France."
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