DOI: 10.1126/science.1095001. TAF1 Activates Transcription by Phosphorylation of Serine 33 in Histone H2B

TAF1 Activates Transcription by Phosphorylation of Serine 33 in Histone H2B

10.1126/science.1095001

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Dynamic changes in chromatin structure, induced by posttranslational modification of histones, play a fundamental role in regulating eukaryotic transcription. Here we report that histone H2B is phosphorylated at evolutionarily conserved Ser 33 (H2B-S33) by the carboxyl-terminal kinase domain (CTK) of the Drosophila TFIID subunit TAF1. Phosphorylation of H2B-S33 at the promoter of the cell cycle regulatory gene string and the segmentation gene giant coincides with transcriptional activation. Elimination of TAF1 CTK activity in Drosophila cells and embryos reduces transcriptional activation and phosphorylation of H2B-S33. These data reveal that H2B-S33 is a physiological substrate for the TAF1 CTK and that H2B-S33 phosphorylation is essential for transcriptional activation events that promote cell cycle progression and development.

Bibliography

Maile, T., Kwoczynski, S., Katzenberger, R. J., Wassarman, D. A., & Sauer, F. (2004). TAF1 Activates Transcription by Phosphorylation of Serine 33 in Histone H2B. Science, 304(5673), 1010â1014.

Authors 5

Tobias Maile (first)
Simona Kwoczynski (additional)
Rebeccah J. Katzenberger (additional)
David A. Wassarman (additional)
Frank Sauer (additional)

References 31 Referenced 53

10.1101/gad.831000
10.1016/S0092-8674(02)00655-4
10.1038/38444
10.1101/gad.1013902
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10.1016/S0092-8674(03)00355-6
10.1146/annurev.bi.48.070179.001111
Materials and methods are available as supporting material on Science Online.
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10.1242/jcs.114.16.2895
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Single-letter abbreviations for the amino acid residues are as follows: A Ala; C Cys; D Asp; E Glu; F Phe; G Gly; H His; I Ile; K Lys; L Leu; M Met; N Asn; P Pro; Q Gln; R Arg; S Ser; T Thr; V Val; W Trp; and Y Tyr.
We thank S. Hansen and R. Tjian for the RAP74 expression plasmid K. Schell and J. Batchelder at the UW-Madison Comprehensive Cancer Flow Cytometry Facility for their assistance and C. Metcalf and T. Sanchez-Elsner for comments that improved the manuscript. This workwas supported by an NIH grant (GM066204-02) to D.A.W. and a Volkswagen Stiftung grant (I/77 996) to F.S.

Dates

Type	When
Created	21 years, 3 months ago (May 13, 2004, 4:18 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:43 p.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 10:18 p.m.)
Issued	21 years, 3 months ago (May 14, 2004)
Published	21 years, 3 months ago (May 14, 2004)
Published Print	21 years, 3 months ago (May 14, 2004)

Funders 0

None

BibTeX

@article{Maile_2004, title={TAF1 Activates Transcription by Phosphorylation of Serine 33 in Histone H2B}, volume={304}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1095001}, DOI={10.1126/science.1095001}, number={5673}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Maile, Tobias and Kwoczynski, Simona and Katzenberger, Rebeccah J. and Wassarman, David A. and Sauer, Frank}, year={2004}, month=may, pages={1010–1014} }

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