DOI: 10.1126/science.1092385. Neutrophil Extracellular Traps Kill Bacteria

Neutrophil Extracellular Traps Kill Bacteria

10.1126/science.1092385

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Neutrophils engulf and kill bacteria when their antimicrobial granules fuse with the phagosome. Here, we describe that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria. These neutrophil extracellular traps (NETs) degrade virulence factors and kill bacteria. NETs are abundant in vivo in experimental dysentery and spontaneous human appendicitis, two examples of acute inflammation. NETs appear to be a form of innate response that binds microorganisms, prevents them from spreading, and ensures a high local concentration of antimicrobial agents to degrade virulence factors and kill bacteria.

Bibliography

Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y., & Zychlinsky, A. (2004). Neutrophil Extracellular Traps Kill Bacteria. Science, 303(5663), 1532â1535.

Authors 8

Volker Brinkmann (first)
Ulrike Reichard (additional)
Christian Goosmann (additional)
Beatrix Fauler (additional)
Yvonne Uhlemann (additional)
David S. Weiss (additional)
Yvette Weinrauch (additional)
Arturo Zychlinsky (additional)

References 21 Referenced 8,048

P. Elsbach, J. Weiss, in Inflammation: Basic Principles and Clinical Correlates, J. I. Gallin, I. M. Goldstein, R. Snyderman, Eds. (Raven Press, New York, ed. 2, 1992), pp. 603–636. / Inflammation: Basic Principles and Clinical Correlates (1992)
S. J. Klebanoff, in Inflammation: Basic Principles and Clinical Correlates, J. I. Gallin, R. Snyderman, Eds. (Lippincott Williams & Wilkens, Philadelphia, PA, ed. 3, 1999), pp. 721–768. / Inflammation: Basic Principles and Clinical Correlates (1999)
P. E. Hensonet al., in Inflammation: Basic Principles and Clinical Correlates, J. I. Gallin, R Snyderman, Eds. (Raven Press, New York, ed. 2, 1992), pp. 511–539. / Inflammation: Basic Principles and Clinical Correlates (1992)
L. C. Junqueira J. Carneiro R. O. Kelley Basic Histology (Appelton & Lange Norwalk CT ed. 8 1995).
10.1016/S0022-1759(99)00173-8
10.1182/blood.V89.10.3503
10.1006/cbir.2001.0760
V. Brinkmann et al. data not shown.
M. J. Losman, T. M. Fasy, K. E. Novick, M. Monestier, J. Immunol.148, 1561 (1992). (10.4049/jimmunol.148.5.1561) / J. Immunol. (1992)
10.1093/nar/25.3.680
10.4049/jimmunol.170.10.5268
10.1182/blood.V92.12.4808
N. A. Maianski, D. Roos, T. W. Kuijpers, Blood101, 1987 (2002). / Blood (2002)
10.1038/417091a
10.1084/jem.108.6.925
S. M. Zhdan-Pushkina, N. V. Dronova, Mikrobiologiia45, 60 (1976). / Mikrobiologiia (1976)
10.1006/bbrc.1996.1814
10.1016/0966-842X(94)90449-9
10.1038/416291a
10.1165/rcmb.2002-0119OC
The gift of M. Monestier Temple University the monoclonal antibody against H2A-H2B-DNA is gratefully acknowledged. The authors thank the help of M. Ingersoll B. Raupach C. Scharff C. Heinz and members of the Department of Cellular Microbiology Max Planck Institute for Infection Biology. Supported in part by NIH grant AI037720.

Dates

Type	When
Created	21 years, 5 months ago (March 4, 2004, 4:23 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:45 p.m.)
Indexed	11 hours, 37 minutes ago (Sept. 1, 2025, 6:32 a.m.)
Issued	21 years, 5 months ago (March 5, 2004)
Published	21 years, 5 months ago (March 5, 2004)
Published Print	21 years, 5 months ago (March 5, 2004)

Funders 0

None

BibTeX

@article{Brinkmann_2004, title={Neutrophil Extracellular Traps Kill Bacteria}, volume={303}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1092385}, DOI={10.1126/science.1092385}, number={5663}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Brinkmann, Volker and Reichard, Ulrike and Goosmann, Christian and Fauler, Beatrix and Uhlemann, Yvonne and Weiss, David S. and Weinrauch, Yvette and Zychlinsky, Arturo}, year={2004}, month=mar, pages={1532–1535} }

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