DOI: 10.1126/science.1091422. The Needle Length of Bacterial Injectisomes Is Determined by a Molecular Ruler

The Needle Length of Bacterial Injectisomes Is Determined by a Molecular Ruler

10.1126/science.1091422

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Size determination represents a fundamental requirement for multicomponent biological structures. Some pathogenic bacteria possess a weapon derived from the flagellum. Like the flagellum, this type-III secretion apparatus, called the injectisome, has a transmembrane basal body, but the external component is a needle-like structure instead of a hook and a filament. Here, we provide evidence that the length of this needle is determined by the size of a protein, YscP, acting as a molecular ruler.

Bibliography

Journet, L., Agrain, C., Broz, P., & Cornelis, G. R. (2003). The Needle Length of Bacterial Injectisomes Is Determined by a Molecular Ruler. Science, 302(5651), 1757â1760.

Authors 4

Laure Journet (first)
Céline Agrain (additional)
Petr Broz (additional)
Guy R. Cornelis (additional)

References 26 Referenced 245

10.1038/nrm932
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T. G. Kimbrough, S. I. Miller, Proc. Natl. Acad. Sci. U.S.A.97, 11008(2000). (10.1073/pnas.200209497) / Proc. Natl. Acad. Sci. U.S.A. (2000)
A. Blocker et al., Mol. Microbiol.39, 652 (2001). (10.1046/j.1365-2958.2001.02200.x) / Mol. Microbiol. (2001)
S. I. Aizawa, FEMS Microbiol. Lett.202, 157 (2001). (10.1111/j.1574-6968.2001.tb10797.x) / FEMS Microbiol. Lett. (2001)
10.1146/annurev.micro.57.030502.090832
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10.1126/science.1058366
K. Tamano, E. Katayama, T. Toyotome, C. Sasakawa, J. Bacteriol.184, 1244 (2002). (10.1128/JB.184.5.1244-1252.2002) / J. Bacteriol. (2002)
J. Magdalena et al., J. Bacteriol.184, 3433 (2002). (10.1128/JB.184.13.3433-3441.2002) / J. Bacteriol. (2002)
Yersinia builds injectisomes when temperature reaches 37°C the host temperature. However Yop secretion is only triggered upon contact with a target cell or artificially by chelating Ca 2+ ions. The usual procedure consists of growing bacteria at 28°C in oxalated rich medium and then switching the culture to 37°C. In these secretion-permissive conditions the production of injectisomes is also stimulated. When bacteria are grown at 37°C in the presence of 5 mM Ca 2+ they make injectisomes but they do not secrete Yops (nonpermissive conditions). Materials and methods are available as supporting material on Science Online.
E. Hoiczyk, G. Blobel, Proc. Natl. Acad. Sci. U.S.A.98, 4669 (2001). (10.1073/pnas.071065798) / Proc. Natl. Acad. Sci. U.S.A. (2001)
I. Stainier et al., Mol. Microbiol.37, 1005 (2000). (10.1046/j.1365-2958.2000.02026.x) / Mol. Microbiol. (2000)
P. L. Payne, S. C. Straley, J. Bacteriol.181, 2852 (1999). (10.1128/JB.181.9.2852-2862.1999) / J. Bacteriol. (1999)
For complementation yscP DNA was amplified by polymerase chain reaction and cloned in the pBAD expression vector. Expression was induced with arabinose.
I. Katsura, Nature327, 73 (1987). (10.1038/327073a0) / Nature (1987)
I. Katsura, R. W. Hendrix, Cell39, 691 (1984). (10.1016/0092-8674(84)90476-8) / Cell (1984)
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T. Minamino, R. M. Macnab, J. Bacteriol.182, 4906 (2000). (10.1128/JB.182.17.4906-4914.2000) / J. Bacteriol. (2000)
P. J. Edqvist et al., J. Bacteriol.185, 2259 (2003). (10.1128/JB.185.7.2259-2266.2003) / J. Bacteriol. (2003)
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F. S. Cordes et al., J. Biol. Chem.278, 17103 (2003). (10.1074/jbc.M300091200) / J. Biol. Chem. (2003)
R. L. Duda, M. Gingery, F. A. Eiserling, Virology151, 296 (1986). (10.1016/0042-6822(86)90051-6) / Virology (1986)
C. M. Collazo, M. K. Zierler, J. E. Galan, Mol. Microbiol.15, 25 (1995). (10.1111/j.1365-2958.1995.tb02218.x) / Mol. Microbiol. (1995)
T. Minamino, B. Gonzalez-Pedrajo, K. Yamaguchi, S. I. Aizawa, R. M. Macnab, Mol. Microbiol.34, 295 (1999). (10.1046/j.1365-2958.1999.01597.x) / Mol. Microbiol. (1999)
We thank M. Duerrenberger for the electron microscopy facility; V. Huschauer for contributing to the needle purification protocol; S. I. Aizawa for advice; M. Kuhn for technical assistance; S. Straley and K. Hughes for supplying yscP pestisKIM and Salmonella LT2; and C. Thompson U. Jenal H. Shin and J. Mota for suggestions. This work was supported by the Swiss National Science Foundation (grant 32-65393.01) and the Swiss Office Fédéral de l'Education et de la Science (European Union Human Potential–Research Training Network CT-2000-00075).

Dates

Type	When
Created	21 years, 8 months ago (Dec. 4, 2003, 5:22 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:43 p.m.)
Indexed	3 months, 1 week ago (May 9, 2025, 10:26 a.m.)
Issued	21 years, 8 months ago (Dec. 5, 2003)
Published	21 years, 8 months ago (Dec. 5, 2003)
Published Print	21 years, 8 months ago (Dec. 5, 2003)

Funders 0

None

BibTeX

@article{Journet_2003, title={The Needle Length of Bacterial Injectisomes Is Determined by a Molecular Ruler}, volume={302}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1091422}, DOI={10.1126/science.1091422}, number={5651}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Journet, Laure and Agrain, Céline and Broz, Petr and Cornelis, Guy R.}, year={2003}, month=dec, pages={1757–1760} }

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