DOI: 10.1126/science.1090284. Three-Dimensional Structure of Herpes Simplex Virus from Cryo-Electron Tomography

Three-Dimensional Structure of Herpes Simplex Virus from Cryo-Electron Tomography

10.1126/science.1090284

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Herpes simplex virus, a DNA virus of high complexity, consists of a nucleocapsid surrounded by the tegument—a protein compartment—and the envelope. The latter components, essential for infectivity, are pleiomorphic. Visualized in cryo–electron tomograms of isolated virions, the tegument was seen to form an asymmetric cap: On one side, the capsid closely approached the envelope; on the other side, they were separated by ∼35 nanometers of tegument. The tegument substructure was particulate, with some short actin-like filaments. The envelope contained 600 to 750 glycoprotein spikes that varied in length, spacing, and in the angles at which they emerge from the membrane. Their distribution was nonrandom, suggesting functional clustering.

Bibliography

GruÌnewald, K., Desai, P., Winkler, D. C., Heymann, J. B., Belnap, D. M., Baumeister, W., & Steven, A. C. (2003). Three-Dimensional Structure of Herpes Simplex Virus from Cryo-Electron Tomography. Science, 302(5649), 1396â1398.

Authors 7

Kay Grünewald (first)
Prashant Desai (additional)
Dennis C. Winkler (additional)
J. Bernard Heymann (additional)
David M. Belnap (additional)
Wolfgang Baumeister (additional)
Alasdair C. Steven (additional)

References 30 Referenced 491

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We thank B. Trus A. Frangakis and O. Medalia for help and advice and gratefully acknowledge contributions from an Emmy Noether fellowship from the Deutsche Forschungsgemeinschaft (K.G.) a Humboldt Foundation fellowship (A.C.S.) the NIH Intramural AIDS Targeted Antiviral Program (A.C.S.) and U.S. Public Health Service grant AI33077 (P.D.) for the support of this research.

Dates

Type	When
Created	21 years, 9 months ago (Nov. 21, 2003, 12:14 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:32 p.m.)
Indexed	3 weeks, 4 days ago (July 26, 2025, 5:07 a.m.)
Issued	21 years, 9 months ago (Nov. 21, 2003)
Published	21 years, 9 months ago (Nov. 21, 2003)
Published Print	21 years, 9 months ago (Nov. 21, 2003)

Funders 0

None

BibTeX

@article{Gru_newald_2003, title={Three-Dimensional Structure of Herpes Simplex Virus from Cryo-Electron Tomography}, volume={302}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1090284}, DOI={10.1126/science.1090284}, number={5649}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Grünewald, Kay and Desai, Prashant and Winkler, Dennis C. and Heymann, J. Bernard and Belnap, David M. and Baumeister, Wolfgang and Steven, Alasdair C.}, year={2003}, month=nov, pages={1396–1398} }

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