DOI: 10.1126/science.1088755. Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins

Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins

10.1126/science.1088755

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An ultrasensitive method for detecting protein analytes has been developed. The system relies on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate-specific antigen (PSA) in this case] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes. Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe. Because the nanoparticle probe carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and PSA can be detected at 30 attomolar concentration. Alternatively, a polymerase chain reaction on the oligonucleotide bar codes can boost the sensitivity to 3 attomolar. Comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of ∼3 picomdar, six orders of magnitude less sensitive than what is observed with this method.

Bibliography

Nam, J.-M., Thaxton, C. S., & Mirkin, C. A. (2003). Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins. Science, 301(5641), 1884â1886.

Authors 3

Jwa-Min Nam (first)
C. Shad Thaxton (additional)
Chad A. Mirkin (additional)

References 27 Referenced 2,210

10.1126/science.2999980
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B. B. Haab M. J. Dunham P. O. Brown Genome Biol.2(2): research0004.1 (2001). (10.1186/gb-2001-2-2-research0004)
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10.1038/382607a0
10.1126/science.297.5586.1536
For 13-nm NPs each NP can support up to 100 strands of DNA with the proteins present ( 26 ); this value represents the upper limit and the exact number for these particles has not yet been determined.
10.1126/science.289.5485.1757
Silver enhancement kit was purchased from Ted Pella (Redding CA) and silver enhancement time was 6 min.
10.1021/ac0006627
C.A.M. acknowledges the Air Force Office of Scientific Research the Defense Advanced Research Projects Agency and the NSF for support of this research. C.S.T. acknowledges the Howard Hughes Medical Institute for a Training fellowship. A. Schaefferis acknowledged for helpful discussions and V. Levenson assisted in PCR optimization.

Dates

Type	When
Created	21 years, 11 months ago (Sept. 25, 2003, 6:06 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 8:58 p.m.)
Indexed	2 days, 11 hours ago (Aug. 31, 2025, 6:03 a.m.)
Issued	21 years, 11 months ago (Sept. 26, 2003)
Published	21 years, 11 months ago (Sept. 26, 2003)
Published Print	21 years, 11 months ago (Sept. 26, 2003)

Funders 0

None

BibTeX

@article{Nam_2003, title={Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins}, volume={301}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1088755}, DOI={10.1126/science.1088755}, number={5641}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nam, Jwa-Min and Thaxton, C. Shad and Mirkin, Chad A.}, year={2003}, month=sep, pages={1884–1886} }

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