DOI: 10.1126/science.1087782. Autophagy Genes Are Essential for Dauer Development and Life-Span Extension in <i>C. elegans</i>

Autophagy Genes Are Essential for Dauer Development and Life-Span Extension in C. elegans

10.1126/science.1087782

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Both dauer formation (a stage of developmental arrest) and adult life-span in Caenorhabditis elegans are negatively regulated by insulin-like signaling, but little is known about cellular pathways that mediate these processes. Autophagy, through the sequestration and delivery of cargo to the lysosomes, is the major route for degrading long-lived proteins and cytoplasmic organelles in eukaryotic cells. Using nematodes with a loss-of-function mutation in the insulin-like signaling pathway, we show that bec-1 , the C. elegans ortholog of the yeast and mammalian autophagy gene APG6/VPS30/beclin1 , is essential for normal dauer morphogenesis and life-span extension. Dauer formation is associated with increased autophagy and also requires C. elegans orthologs of the yeast autophagy genes APG1, APG7, APG8 , and AUT10 . Thus, autophagy is a cellular pathway essential for dauer development and life-span extension in C. elegans.

Bibliography

MeleÌndez, A., TalloÌczy, Z., Seaman, M., Eskelinen, E.-L., Hall, D. H., & Levine, B. (2003). Autophagy Genes Are Essential for Dauer Development and Life-Span Extension in C. elegans. Science, 301(5638), 1387â1391.

Authors 6

Alicia Meléndez (first)
Zsolt Tallóczy (additional)
Matthew Seaman (additional)
Eeva-Liisa Eskelinen (additional)
David H. Hall (additional)
Beth Levine (additional)

References 35 Referenced 1,018

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We thank J. Yu W. Jiang F. Macaluso and G. Stephney for excellent technical assistance; R. Chamberlain for secretarial assistance; A. Fire Y. Kohara and T. Stiernagle for providing reagents; and H. Bülow for helpful discussions. We are particularly grateful to I. Greenwald for generous support. Supported by NIH grant nos. RO1 CA84254 (B.L. and A.M.) and RR 12596 (D.H.H.) American Cancer Society grant no. RSG-339 (B.L) and the Medical Research Council of the UK (M.S.).

Dates

Type	When
Created	21 years, 11 months ago (Sept. 4, 2003, 4:43 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:28 p.m.)
Indexed	39 minutes ago (Aug. 28, 2025, 8:38 a.m.)
Issued	21 years, 11 months ago (Sept. 5, 2003)
Published	21 years, 11 months ago (Sept. 5, 2003)
Published Print	21 years, 11 months ago (Sept. 5, 2003)

Funders 0

None

BibTeX

@article{Mele_ndez_2003, title={Autophagy Genes Are Essential for Dauer Development and Life-Span Extension in C. elegans}, volume={301}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1087782}, DOI={10.1126/science.1087782}, number={5638}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Meléndez, Alicia and Tallóczy, Zsolt and Seaman, Matthew and Eskelinen, Eeva-Liisa and Hall, David H. and Levine, Beth}, year={2003}, month=sep, pages={1387–1391} }

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      "unstructured": "We thank J. Yu W. Jiang F. Macaluso and G. Stephney for excellent technical assistance; R. Chamberlain for secretarial assistance; A. Fire Y. Kohara and T. Stiernagle for providing reagents; and H. B\u00fclow for helpful discussions. We are particularly grateful to I. Greenwald for generous support. Supported by NIH grant nos. RO1 CA84254 (B.L. and A.M.) and RR 12596 (D.H.H.) American Cancer Society grant no. RSG-339 (B.L) and the Medical Research Council of the UK (M.S.)."
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