DOI: 10.1126/science.1086957. Untangling Desmosomal Knots with Electron Tomography

Untangling Desmosomal Knots with Electron Tomography

10.1126/science.1086957

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cell adhesion by adherens junctions and desmosomes relies on interactions between cadherin molecules. However, the molecular interfaces that define molecular specificity and that mediate adhesion remain controversial. We used electron tomography of plastic sections from neonatal mouse skin to visualize the organization of desmosomes in situ. The resulting three-dimensional maps reveal individual cadherin molecules forming discrete groups and interacting through their tips. Fitting of an x-ray crystal structure for C-cadherin to these maps is consistent with a flexible intermolecular interface mediated by an exchange of amino-terminal tryptophans. This flexibility suggests a novel mechanism for generating both cis and trans interactions and for propagating these adhesive interactions along the junction.

Bibliography

He, W., Cowin, P., & Stokes, D. L. (2003). Untangling Desmosomal Knots with Electron Tomography. Science, 302(5642), 109â113.

Authors 3

Wanzhong He (first)
Pamela Cowin (additional)
David L. Stokes (additional)

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We thank Y. Ji M. Auer and K. MacDonald for help in preparing samples; F. Macaluso and J. Ault for assistance with high-pressure freezing; and K. Taylor E. Branlund N. Kisseberth and D. Mastronarde for providing facilities and support for tomographic data collection and image reconstruction. Coordinates for Fig. 2 G to I have been deposited in the Protein Data Bank with accession codes 1Q55 1Q5A 1Q5B and 1Q5C. Tomographic reconstructions have been submitted to the electron microscopy database of the European Bioinformatics Institute with accession codes EMD-1051 EMD-1052 and EMD-1053. Supported by NIH grant R01 GM47429 (P.C.).

Dates

Type	When
Created	21 years, 10 months ago (Oct. 2, 2003, 4:43 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 10:01 p.m.)
Indexed	2 months, 1 week ago (June 13, 2025, 6:25 a.m.)
Issued	21 years, 10 months ago (Oct. 3, 2003)
Published	21 years, 10 months ago (Oct. 3, 2003)
Published Print	21 years, 10 months ago (Oct. 3, 2003)

Funders 0

None

BibTeX

@article{He_2003, title={Untangling Desmosomal Knots with Electron Tomography}, volume={302}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1086957}, DOI={10.1126/science.1086957}, number={5642}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={He, Wanzhong and Cowin, Pamela and Stokes, David L.}, year={2003}, month=oct, pages={109–113} }

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