DOI: 10.1126/science.1086391. Genome-Wide Insertional Mutagenesis of <i>Arabidopsis thaliana</i>

Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

10.1126/science.1086391

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ∼29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.

Bibliography

Alonso, J. M., Stepanova, A. N., Leisse, T. J., Kim, C. J., Chen, H., Shinn, P., Stevenson, D. K., Zimmerman, J., Barajas, P., Cheuk, R., Gadrinab, C., Heller, C., Jeske, A., Koesema, E., Meyers, C. C., Parker, H., Prednis, L., Ansari, Y., Choy, N., â¦ Ecker, J. R. (2003). Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana. Science, 301(5633), 653â657.

Authors 39

José M. Alonso (first)
Anna N. Stepanova (additional)
Thomas J. Leisse (additional)
Christopher J. Kim (additional)
Huaming Chen (additional)
Paul Shinn (additional)
Denise K. Stevenson (additional)
Justin Zimmerman (additional)
Pascual Barajas (additional)
Rosa Cheuk (additional)
Carmelita Gadrinab (additional)
Collen Heller (additional)
Albert Jeske (additional)
Eric Koesema (additional)
Cristina C. Meyers (additional)
Holly Parker (additional)
Lance Prednis (additional)
Yasser Ansari (additional)
Nathan Choy (additional)
Hashim Deen (additional)
Michael Geralt (additional)
Nisha Hazari (additional)
Emily Hom (additional)
Meagan Karnes (additional)
Celene Mulholland (additional)
Ral Ndubaku (additional)
Ian Schmidt (additional)
Plinio Guzman (additional)
Laura Aguilar-Henonin (additional)
Markus Schmid (additional)
Detlef Weigel (additional)
David E. Carter (additional)
Trudy Marchand (additional)
Eddy Risseeuw (additional)
Debra Brogden (additional)
Albana Zeko (additional)
William L. Crosby (additional)
Charles C. Berry (additional)
Joseph R. Ecker (additional)

References 28 Referenced 4,236

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We thank A. Hamilton and members of the Ecker laboratory for critical reading of the manuscript and stimulating discussions. J.M.A. was in part supported by a postdoctoral fellowship from the Spanish Ministerio de Educación y Ciencia. A.N.S. was a recipient of a predoctoral fellowship from the Department of Energy (ER20162-A0003). The W.L.C. laboratory was supported by a grant from the Canadian National Research Council Vice-President's fund. P. G. was on a sabbatical recess from Cinvestav-Irapuato Mexico and was supported in part by a fellowship from CONACYT Mexico. We thank K. Davis and colleagues at Paradigm Genetics for their assistance in bulking up low-seed T1 lines. This work was supported by an NSF Arabidopsis 2010 project grant (MCB O115103) and Department of Energy DE-FG03-00ER15113 awarded to J.R.E. Seeds for all the T-DNA lines are publicly available from The Arabidopsis Biological Resource Center at Ohio State University (http://arabidopsis.org/abrc/). Affymetrix microarray expression data has been deposited in the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). GEO accessions: GSM8467 through GSM8478.

Dates

Type	When
Created	22 years, 1 month ago (July 31, 2003, 11:27 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:41 p.m.)
Indexed	47 minutes ago (Sept. 6, 2025, 8:40 a.m.)
Issued	22 years, 1 month ago (Aug. 1, 2003)
Published	22 years, 1 month ago (Aug. 1, 2003)
Published Print	22 years, 1 month ago (Aug. 1, 2003)

Funders 0

None

BibTeX

@article{Alonso_2003, title={Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana}, volume={301}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1086391}, DOI={10.1126/science.1086391}, number={5633}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Alonso, José M. and Stepanova, Anna N. and Leisse, Thomas J. and Kim, Christopher J. and Chen, Huaming and Shinn, Paul and Stevenson, Denise K. and Zimmerman, Justin and Barajas, Pascual and Cheuk, Rosa and Gadrinab, Carmelita and Heller, Collen and Jeske, Albert and Koesema, Eric and Meyers, Cristina C. and Parker, Holly and Prednis, Lance and Ansari, Yasser and Choy, Nathan and Deen, Hashim and Geralt, Michael and Hazari, Nisha and Hom, Emily and Karnes, Meagan and Mulholland, Celene and Ndubaku, Ral and Schmidt, Ian and Guzman, Plinio and Aguilar-Henonin, Laura and Schmid, Markus and Weigel, Detlef and Carter, David E. and Marchand, Trudy and Risseeuw, Eddy and Brogden, Debra and Zeko, Albana and Crosby, William L. and Berry, Charles C. and Ecker, Joseph R.}, year={2003}, month=aug, pages={653–657} }

JSON

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  "abstract": "<jats:p>\n            Over 225,000 independent\n            <jats:italic>Agrobacterium</jats:italic>\n            transferred DNA (T-DNA) insertion events in the genome of the reference plant\n            <jats:italic>Arabidopsis thaliana</jats:italic>\n            have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the \u223c29,454 predicted\n            <jats:italic>Arabidopsis</jats:italic>\n            genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.\n          </jats:p>",
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