DOI: 10.1126/science.1084909. Fusion of Cells by Flipped SNAREs

10.1126/science.1084909

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The SNARE (soluble N -ethylmaleimide–sensitive factor attachment protein receptor) hypothesis suggests that pairs of proteins known as vesicle (v-) SNAREs and target membrane (t-) SNAREs interact specifically to control and mediate intracellular membrane fusion events. Here, cells expressing the interacting domains of v- and t-SNAREs on the cell surface were found to fuse spontaneously, demonstrating that SNAREs are sufficient to fuse biological membranes.

Bibliography

Hu, C., Ahmed, M., Melia, T. J., SoÌllner, T. H., Mayer, T., & Rothman, J. E. (2003). Fusion of Cells by Flipped SNAREs. Science, 300(5626), 1745â1749.

Authors 6

Chuan Hu (first)
Mahiuddin Ahmed (additional)
Thomas J. Melia (additional)
Thomas H. Söllner (additional)
Thomas Mayer (additional)
James E. Rothman (additional)

References 31 Referenced 194

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E. Witze, J. H. Rothman, Curr. Biol.12, R 467 (2002). (10.1016/S0960-9822(02)00712-1) / Curr. Biol. (2002)
We thank the Molecular Cytology Core Facility of the Memorial Sloan-Kettering Cancer Center for assistance with microscopy; P. Antinozzi L. Mahal and other members of the Rothman lab for helpful discussions; W. Eng for technical support; and B. V. Zemelman for plasmids. Supported by a grant from the NIH (J.E.R.).

Dates

Type	When
Created	22 years, 2 months ago (June 12, 2003, 8:13 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 11:34 p.m.)
Indexed	1 month, 1 week ago (July 19, 2025, 11:45 p.m.)
Issued	22 years, 2 months ago (June 13, 2003)
Published	22 years, 2 months ago (June 13, 2003)
Published Print	22 years, 2 months ago (June 13, 2003)

Funders 0

None

BibTeX

@article{Hu_2003, title={Fusion of Cells by Flipped SNAREs}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1084909}, DOI={10.1126/science.1084909}, number={5626}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hu, Chuan and Ahmed, Mahiuddin and Melia, Thomas J. and Söllner, Thomas H. and Mayer, Thomas and Rothman, James E.}, year={2003}, month=jun, pages={1745–1749} }

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