DOI: 10.1126/science.1084398. Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization [Penn]

Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization

10.1126/science.1084398

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ∼37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 – 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.

Bibliography

Yildiz, A., Forkey, J. N., McKinney, S. A., Ha, T., Goldman, Y. E., & Selvin, P. R. (2003). Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization. Science, 300(5628), 2061â2065.

Authors 6

Ahmet Yildiz (first)
Joseph N. Forkey (additional)
Sean A. McKinney (additional)
Taekjip Ha (additional)
Yale E. Goldman (additional)
Paul R. Selvin (additional)

References 33 Referenced 1,607

10.1038/23072
10.1038/ncb732
10.1242/jcs.114.11.1981
10.1016/S0167-4889(00)00007-0
10.1073/pnas.182539599
10.1038/nature01529
10.1073/pnas.96.24.13726
10.1126/science.1063089
10.1038/nsb803
10.1073/pnas.191512398
10.1016/S0091-679X(08)60982-6
10.1038/374555a0
10.1006/bbrc.1997.6732
Materials and Methods are available as supporting online material on Science Online.
10.1073/pnas.93.7.2926
10.1016/S0006-3495(00)76764-6
10.1073/pnas.170286097
10.1016/S0006-3495(02)75618-X
10.1038/331450a0
10.1063/1.1138619
10.1016/S0006-3495(01)75884-5
10.1016/S0022-2836(05)80060-9
10.1073/pnas.120174297
10.1126/science.286.5445.1722
10.1038/22704
10.1006/bbrc.2000.2819
10.1021/jp9846330
Fitting the data to two rate constants does not improve the fit; however the curve is fit equally well to a range of (k 1 k 2 ) where k 1 and k 2 can differ by a factor of 2 or more. Hence we cannot rule out asymmetry in the rates of the two heads.
10.1038/35015592
10.1073/pnas.97.17.9482
10.1083/jcb.200208172
10.1038/35073086
Supported by NIH grants AR44420 (to P.R.S.) GM65367 (to T.H.) and AR26846 (to Y.E.G.); NSF grants DBI-02-15869 (to P.R.S. and T.H.) and 9984841 (to P.R.S.); the Carver Trust Foundation (to P.R.S.); and the DOE Division of Materials Sciences (under award no. DEFG02-91ER45439) through the Frederick Seitz Materials Research Laboratory at the University of Illinois at Urbana-Champaign (to P.R.S.). S.A.M. was supported by a National Research Service Award in Molecular Biophysics (through NIH training grant PHS 5 T32 GM08276). We thank J. E. T. Corrie for gift of bis-rhodamine E. Gratton for initial suggestions and discussions B. C. Stevens for computer programming assistance and I. Rasnik for fluorescence imaging assistance.

Dates

Type	When
Created	22 years, 2 months ago (June 9, 2003, 8:22 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 11:41 p.m.)
Indexed	4 days, 16 hours ago (Aug. 20, 2025, 8:31 a.m.)
Issued	22 years, 1 month ago (June 27, 2003)
Published	22 years, 1 month ago (June 27, 2003)
Published Print	22 years, 1 month ago (June 27, 2003)

Funders 0

None

BibTeX

@article{Yildiz_2003, title={Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1084398}, DOI={10.1126/science.1084398}, number={5628}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yildiz, Ahmet and Forkey, Joseph N. and McKinney, Sean A. and Ha, Taekjip and Goldman, Yale E. and Selvin, Paul R.}, year={2003}, month=jun, pages={2061–2065} }

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