DOI: 10.1126/science.1084274. Role of Histone H3 Lysine 27 Methylation in X Inactivation

Role of Histone H3 Lysine 27 Methylation in X Inactivation

10.1126/science.1084274

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation in extraembryonic cells but not of random X inactivation in embryonic cells. The Drosophila homolog of the Eed-Ezh2 PcG protein complex achieves gene silencing through methylation of histone H3 on lysine 27 (H3-K27), which suggests a role for H3-K27 methylation in imprinted X inactivation. Here we demonstrate that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation. Recruitment of the complex and methylation on the Xi depend on Xist RNA but are independent of its silencing function. Together, our results suggest a role for Eed-Ezh2–mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi.

Bibliography

Plath, K., Fang, J., Mlynarczyk-Evans, S. K., Cao, R., Worringer, K. A., Wang, H., de la Cruz, C. C., Otte, A. P., Panning, B., & Zhang, Y. (2003). Role of Histone H3 Lysine 27 Methylation in X Inactivation. Science, 300(5616), 131â135.

Authors 10

Kathrin Plath (first)
Jia Fang (additional)
Susanna K. Mlynarczyk-Evans (additional)
Ru Cao (additional)
Kathleen A. Worringer (additional)
Hengbin Wang (additional)
Cecile C. de la Cruz (additional)
Arie P. Otte (additional)
Barbara Panning (additional)
Yi Zhang (additional)

References 23 Referenced 994

10.1038/35047580
10.1146/annurev.genet.36.042902.092433
10.1093/hmg/10.20.2225
10.1016/S0168-9525(02)02717-8
10.1101/gad.927301
10.1016/S0959-437X(02)00287-3
10.1126/science.1063127
10.1126/science.1076997
10.1016/S0092-8674(02)00975-3
10.1101/gad.1035902
10.1016/S0092-8674(02)00976-5
10.1038/ng574
10.1016/S0960-9822(02)00892-8
R. Cao and Y. Zhang unpublished results.
K. A. Worringer and B. Panning unpublished data.
K. Plath and B. Panning unpublished results.
___ unpublished results.
10.1038/ng820
10.1016/S0959-437X(02)00288-5
10.1038/384648a0
10.1016/S1097-2765(00)80248-8
B. Panning unpublished data.
We thank G. Uy R. Jaenisch and T. Magnusonfor reagents; V. Nguyen and D. Scheel for preimplantation embryos; A. Andersen D. Nusinow and H. Cohen for critical reading of the manuscript. Y.Z. is a Kimmel Scholar and is supported by grants from NIH and the American Chemical Society. B.P. is a Pew Scholar and is funded by NIH and by grants from Howard Hughes Medical Institute and the Sandler Family Foundation. K.P. is an O'Donnell Foundation Fellow of the Life Sciences Research Foundation. S.M.-E. and C.C.C. are supported by a National Sciences Foundation graduate fellowship.

Dates

Type	When
Created	22 years, 4 months ago (April 4, 2003, 12:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:13 p.m.)
Indexed	2 days, 14 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	22 years, 4 months ago (April 4, 2003)
Published	22 years, 4 months ago (April 4, 2003)
Published Print	22 years, 4 months ago (April 4, 2003)

Funders 0

None

BibTeX

@article{Plath_2003, title={Role of Histone H3 Lysine 27 Methylation in X Inactivation}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1084274}, DOI={10.1126/science.1084274}, number={5616}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Plath, Kathrin and Fang, Jia and Mlynarczyk-Evans, Susanna K. and Cao, Ru and Worringer, Kathleen A. and Wang, Hengbin and de la Cruz, Cecile C. and Otte, Arie P. and Panning, Barbara and Zhang, Yi}, year={2003}, month=apr, pages={131–135} }

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