Molecular Architecture of the Multiprotein Splicing Factor SF3b
10.1126/science.1084155
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The splicing factor SF3b is a multiprotein complex essential for the accurate excision of introns from pre-messenger RNA. As an integral component of the U2 small nuclear ribonucleoprotein (snRNP) and the U11/U12 di-snRNP, SF3b is involved in the recognition of the pre-messenger RNA's branch site within the major and minor spliceosomes. We have determined the three-dimensional structure of the human SF3b complex by single-particle electron cryomicroscopy at a resolution of less than 10 angstroms, allowing identification of protein domains with known structural folds. The best fit of a modeled RNA-recognition motif indicates that the protein p14 is located in the central cavity of the complex. The 22 tandem helical repeats of the protein SF3b155 are located in the outer shell of the complex enclosing p14.

Bibliography

Golas, M. M., Sander, B., Will, C. L., Lührmann, R., & Stark, H. (2003). Molecular Architecture of the Multiprotein Splicing Factor SF3b. Science, 300(5621), 980–984.

Authors 5
  1. Monika M. Golas (first)
  2. Bjoern Sander (additional)
  3. Cindy L. Will (additional)
  4. Reinhard Lührmann (additional)
  5. Holger Stark (additional)
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  32. We thank G. Heyne for expert technical assistance. Supported bygrants from the Bundesministerium für Bildung Wissenschaft Forschung und Technologie to R.L. and H.S. and the Deutsche Forschungsgemeinschaft to R.L.
Dates
Type When
Created 22 years, 3 months ago (May 8, 2003, 4:53 p.m.)
Deposited 1 year, 7 months ago (Jan. 9, 2024, 11:26 p.m.)
Indexed 1 week, 4 days ago (Aug. 21, 2025, 12:59 p.m.)
Issued 22 years, 3 months ago (May 9, 2003)
Published 22 years, 3 months ago (May 9, 2003)
Published Print 22 years, 3 months ago (May 9, 2003)
Funders 0

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@article{Golas_2003, title={Molecular Architecture of the Multiprotein Splicing Factor SF3b}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1084155}, DOI={10.1126/science.1084155}, number={5621}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Golas, Monika M. and Sander, Bjoern and Will, Cindy L. and Lührmann, Reinhard and Stark, Holger}, year={2003}, month=may, pages={980–984} }