Recombinant Antibodies to the Small GTPase Rab6 as Conformation Sensors
10.1126/science.1083911
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Here we report an approach, based on antibody phage display, to generate molecular conformation sensors. Recombinant antibodies specific to the guanosine triphosphate (GTP)–bound conformation of the small guanosine triphosphatase (GTPase) Rab6, a regulator of membrane traffic, were generated and used to locate Rab6·GTP in fixed cells, and, after green fluorescent protein (GFP) tagging and intracellular expression, to follow Rab6·GTP in vivo. Rab6 was in its GTP-bound conformation on the Golgi apparatus and transport intermediates, and the geometry of transport intermediates was modulated by Rab6 activity. More generally, the same approach could be applied to other molecules that can be locked in a particular conformation in vitro.

Bibliography

Nizak, C., Monier, S., del Nery, E., Moutel, S., Goud, B., & Perez, F. (2003). Recombinant Antibodies to the Small GTPase Rab6 as Conformation Sensors. Science, 300(5621), 984–987.

Authors 6
  1. Clément Nizak (first)
  2. Solange Monier (additional)
  3. Elaine del Nery (additional)
  4. Sandrine Moutel (additional)
  5. Bruno Goud (additional)
  6. Franck Perez (additional)
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Dates
Type When
Created 22 years, 3 months ago (May 8, 2003, 4:53 p.m.)
Deposited 1 year, 7 months ago (Jan. 9, 2024, 11:26 p.m.)
Indexed 1 week ago (Aug. 23, 2025, 12:59 a.m.)
Issued 22 years, 3 months ago (May 9, 2003)
Published 22 years, 3 months ago (May 9, 2003)
Published Print 22 years, 3 months ago (May 9, 2003)
Funders 0

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@article{Nizak_2003, title={Recombinant Antibodies to the Small GTPase Rab6 as Conformation Sensors}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1083911}, DOI={10.1126/science.1083911}, number={5621}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nizak, Clément and Monier, Solange and del Nery, Elaine and Moutel, Sandrine and Goud, Bruno and Perez, Franck}, year={2003}, month=may, pages={984–987} }