DOI: 10.1126/science.1082374. Closing of the Nucleotide Pocket of Kinesin-Family Motors upon Binding to Microtubules

Closing of the Nucleotide Pocket of Kinesin-Family Motors upon Binding to Microtubules

10.1126/science.1082374

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

We have used adenosine diphosphate analogs containing electron paramagnetic resonance (EPR) spin moieties and EPR spectroscopy to show that the nucleotide-binding site of kinesin-family motors closes when the motor·diphosphate complex binds to microtubules. Structural analyses demonstrate that a domain movement in the switch 1 region at the nucleotide site, homologous to domain movements in the switch 1 region in the G proteins [heterotrimeric guanine nucleotide–binding proteins], explains the EPR data. The switch movement primes the motor both for the free energy–yielding nucleotide hydrolysis reaction and for subsequent conformational changes that are crucial for the generation of force and directed motion along the microtubule.

Bibliography

Naber, N., Minehardt, T. J., Rice, S., Chen, X., Grammer, J., Matuska, M., Vale, R. D., Kollman, P. A., Car, R., Yount, R. G., Cooke, R., & Pate, E. (2003). Closing of the Nucleotide Pocket of Kinesin-Family Motors upon Binding to Microtubules. Science, 300(5620), 798â801.

Authors 12

Nariman Naber (first)
Todd J. Minehardt (additional)
Sarah Rice (additional)
Xiaoru Chen (additional)
Jean Grammer (additional)
Marija Matuska (additional)
Ronald D. Vale (additional)
Peter A. Kollman (additional)
Roberto Car (additional)
Ralph G. Yount (additional)
Roger Cooke (additional)
Edward Pate (additional)

References 30 Referenced 47

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This work was supported by NIH grants AR39643 (E.P.) AR42895 (R.C. R.D.V.) DK05915 (R.G.Y.) and GM29072 (P.A.K.). T.J.M. was a Princeton University Councilon Science and Technology Postdoctoral Fellow. Molecular visualizations were done using the UCSF Computer Graphics Laboratory supported by NIH grant RR1081 to T. Ferrin.

Dates

Type	When
Created	22 years, 3 months ago (May 1, 2003, 4:53 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 11:20 p.m.)
Indexed	1 day, 8 hours ago (Aug. 29, 2025, 6:35 a.m.)
Issued	22 years, 3 months ago (May 2, 2003)
Published	22 years, 3 months ago (May 2, 2003)
Published Print	22 years, 3 months ago (May 2, 2003)

Funders 0

None

BibTeX

@article{Naber_2003, title={Closing of the Nucleotide Pocket of Kinesin-Family Motors upon Binding to Microtubules}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1082374}, DOI={10.1126/science.1082374}, number={5620}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Naber, Nariman and Minehardt, Todd J. and Rice, Sarah and Chen, Xiaoru and Grammer, Jean and Matuska, Marija and Vale, Ronald D. and Kollman, Peter A. and Car, Roberto and Yount, Ralph G. and Cooke, Roger and Pate, Edward}, year={2003}, month=may, pages={798–801} }

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