DOI: 10.1126/science.1082320. Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies

Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies

10.1126/science.1082320

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A major pathway of eukaryotic messenger RNA (mRNA) turnover begins with deadenylation, followed by decapping and 5′ to 3′ exonucleolytic decay. We provide evidence that mRNA decapping and 5′ to 3′ degradation occur in discrete cytoplasmic foci in yeast, which we call processing bodies (P bodies). First, proteins that activate or catalyze decapping are concentrated in P bodies. Second, inhibiting mRNA turnover before decapping leads to loss of P bodies; however, inhibiting turnover at, or after, decapping, increases the abundance and size of P bodies. Finally, mRNA degradation intermediates are localized to P bodies. These results define the fluxof mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation.

Bibliography

Sheth, U., & Parker, R. (2003). Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies. Science, 300(5620), 805â808.

Authors 2

Ujwal Sheth (first)
Roy Parker (additional)

References 35 Referenced 1,147

10.1101/gad.5.2.221
10.1101/gad.7.8.1632
10.1101/gad.6.11.2100
10.1101/gad.8.7.855
10.1128/MCB.15.4.2145
10.1038/382642a0
10.1093/emboj/18.19.5411
C. L. Hsu, A. Stevens, Mol. Cell. Biol.13, 4826 (1993). / Mol. Cell. Biol. (1993)
10.1146/annurev.biochem.69.1.571
10.1128/MCB.19.8.5247
10.1128/MCB.20.21.7933-7942.2000
10.1016/S1097-2765(01)00395-1
10.1017/S135583820101994X
10.1093/nar/25.5.965
10.1242/dev.128.17.3233
10.1242/dev.128.17.3221
10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U
The GFP-tagged fusions of decay factors were shown to be functional in vivo for mRNA decay by comparing the ratio of the full length to the decay fragment at steady state of PGK1pG and MFA2pG reporter mRNAs.
10.1038/35006676
U. Sheth R. Parker unpublished observations.
10.1016/S0092-8674(01)00225-2
10.1128/MCB.20.21.8230-8243.2000
10.1093/emboj/19.23.6602
10.1128/MCB.20.16.5939-5946.2000
10.1093/emboj/19.7.1661
10.1016/S0021-9258(17)36937-5
10.1016/S1097-2765(00)80143-4
10.1093/emboj/cdf678
10.1128/MCB.22.23.8114-8121.2002
D. Ingelfinger, D. J. Arndt-Jovin, R. Luhrmann, T. Achsel, RNA8, 1489 (2002). (10.1017/S1355838202021726) / RNA (2002)
M. Brengues R. Parker unpublished observation.
T. LaGrandeur, R. Parker, RNA5, 4420 (1999). / RNA (1999)
10.1242/jcs.115.16.3227
10.1091/mbc.01-05-0221
We thank M. A. V. Sanchez for the MFA2-pG-MS2 plasmid P. Hilleren for the PGK1-pG-MS2 plasmid K. Bloom for the MS2-GFP plasmid A. van Hoof for the strain expressing SKI7-GFP R. Tsein for the mRFP plasmid H. Fares for experimental assistance J. M. Coller for helpful discussions and the members of the Parker Lab for their advice. Supported by Howard Hughes Medical Institute and NIH (GM45443).

Dates

Type	When
Created	22 years, 3 months ago (May 1, 2003, 12:53 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 6:21 p.m.)
Indexed	14 hours, 27 minutes ago (Aug. 28, 2025, 8:42 a.m.)
Issued	22 years, 3 months ago (May 2, 2003)
Published	22 years, 3 months ago (May 2, 2003)
Published Print	22 years, 3 months ago (May 2, 2003)

Funders 0

None

BibTeX

@article{Sheth_2003, title={Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1082320}, DOI={10.1126/science.1082320}, number={5620}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sheth, Ujwal and Parker, Roy}, year={2003}, month=may, pages={805–808} }

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