DOI: 10.1126/science.1082160. Light Microscopy Techniques for Live Cell Imaging

Light Microscopy Techniques for Live Cell Imaging

10.1126/science.1082160

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Since the earliest examination of cellular structures, biologists have been fascinated by observing cells using light microscopy. The advent of fluorescent labeling technologies plus the plethora of sophisticated light microscope techniques now available make studying dynamic processes in living cells almost commonplace. For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to give a brief overview of the main approaches to live cell imaging, with some mention of their pros and cons.

Bibliography

Stephens, D. J., & Allan, V. J. (2003). Light Microscopy Techniques for Live Cell Imaging. Science, 300(5616), 82â86.

Authors 2

David J. Stephens (first)
Victoria J. Allan (additional)

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Materials and methods are available as supporting material on Science Online.
D.J.S. is supported by a Research Career Development Award from the Medical Research Council (grant G120/617). Figure 2 was produced with the use of equipment provided by the Biotechnology and Biological Sciences Research Council UK Bioimaging Initiative (grant C11195) and the Wellcome Trust (grant 043846) to V.J.A.

Dates

Type	When
Created	22 years, 4 months ago (April 4, 2003, 12:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:14 p.m.)
Indexed	2 days, 10 hours ago (Aug. 23, 2025, 9:20 p.m.)
Issued	22 years, 4 months ago (April 4, 2003)
Published	22 years, 4 months ago (April 4, 2003)
Published Print	22 years, 4 months ago (April 4, 2003)

Funders 0

None

BibTeX

@article{Stephens_2003, title={Light Microscopy Techniques for Live Cell Imaging}, volume={300}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1082160}, DOI={10.1126/science.1082160}, number={5616}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Stephens, David J. and Allan, Victoria J.}, year={2003}, month=apr, pages={82–86} }

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