DOI: 10.1126/science.1081412. Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor

Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor

10.1126/science.1081412

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.

Bibliography

Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., & Mitchison, T. J. (2003). Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor. Science, 299(5613), 1743â1747.

Authors 7

Aaron F. Straight (first)
Amy Cheung (additional)
John Limouze (additional)
Irene Chen (additional)
Nick J. Westwood (additional)
James R. Sellers (additional)
Timothy J. Mitchison (additional)

References 34 Referenced 1,242

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We thank E. M. Ostap for purified myosin Ib; J. Dantzig A. Shaw and Y. M. Goldman for rabbit skeletal myosin SI; L. Flanagan and T. Stossel for M2 cells; K. Pierce and M. M.-C. Lofor assistance with chiral chromatography; S. Miller and T. Kapoor for advice on chemical synthesis; T. Kapoor for assistance with aurora kinase inhibitors; and A. Farrell W. Brieher and R. Ward for stimulating discussion and critical review of the manuscript. This work was supported by grants from the NIH (GM62566 GM23928) to T.J.M. and from Merck & Co. and E. Merck. A.F.S. was supported by the Cancer Research Fund of the Damon Runyon–Walter Winchell Foundation.

Dates

Type	When
Created	22 years, 5 months ago (March 13, 2003, 4:43 p.m.)
Deposited	8 months, 2 weeks ago (Dec. 11, 2024, 6:19 p.m.)
Indexed	4 days, 2 hours ago (Aug. 28, 2025, 8:52 a.m.)
Issued	22 years, 5 months ago (March 14, 2003)
Published	22 years, 5 months ago (March 14, 2003)
Published Print	22 years, 5 months ago (March 14, 2003)

Funders 0

None

BibTeX

@article{Straight_2003, title={Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor}, volume={299}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1081412}, DOI={10.1126/science.1081412}, number={5613}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Straight, Aaron F. and Cheung, Amy and Limouze, John and Chen, Irene and Westwood, Nick J. and Sellers, James R. and Mitchison, Timothy J.}, year={2003}, month=mar, pages={1743–1747} }

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