DOI: 10.1126/science.1079237. Dispelling the Myths--Biocatalysis in Industrial Synthesis

Dispelling the Myths--Biocatalysis in Industrial Synthesis

10.1126/science.1079237

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Biocatalysis has emerged as an important tool in the industrial synthesis of bulk chemicals, pharmaceutical and agrochemical intermediates, active pharmaceuticals, and food ingredients. However, the number and diversity of the applications are modest, perhaps in part because of perceived or real limitations of biocatalysts, such as limited enzyme availability, substrate scope, and operational stability. Recent scientific breakthroughs in genomics, directed enzyme evolution, and the exploitation of biodiversity should help to overcome these limitations. As a result, we expect many new industrial applications of biocatalysis to be realized, from single-step enzymatic conversions to customized multistep microbial synthesis by means of metabolic pathway engineering.

Authors 3

Hans E. Schoemaker (first)
Daniel Mink (additional)
Marcel G. Wubbolts (additional)

References 43 Referenced 739

Dates

Type	When
Created	22 years, 5 months ago (March 13, 2003, 4:43 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:43 p.m.)
Indexed	6 days, 11 hours ago (Aug. 21, 2025, 12:50 p.m.)
Issued	22 years, 5 months ago (March 14, 2003)
Published	22 years, 5 months ago (March 14, 2003)
Published Print	22 years, 5 months ago (March 14, 2003)

Funders 0

None

BibTeX

@article{Schoemaker_2003, title={Dispelling the Myths--Biocatalysis in Industrial Synthesis}, volume={299}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1079237}, DOI={10.1126/science.1079237}, number={5613}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schoemaker, Hans E. and Mink, Daniel and Wubbolts, Marcel G.}, year={2003}, month=mar, pages={1694–1697} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T16:50:33Z",
    "timestamp": 1755795033835
  },
  "reference-count": 43,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5613",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        3,
        14
      ]
    ]
  },
  "abstract": "<jats:p>Biocatalysis has emerged as an important tool in the industrial synthesis of bulk chemicals, pharmaceutical and agrochemical intermediates, active pharmaceuticals, and food ingredients. However, the number and diversity of the applications are modest, perhaps in part because of perceived or real limitations of biocatalysts, such as limited enzyme availability, substrate scope, and operational stability. Recent scientific breakthroughs in genomics, directed enzyme evolution, and the exploitation of biodiversity should help to overcome these limitations. As a result, we expect many new industrial applications of biocatalysis to be realized, from single-step enzymatic conversions to customized multistep microbial synthesis by means of metabolic pathway engineering.</jats:p>",
  "DOI": "10.1126/science.1079237",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        13
      ]
    ],
    "date-time": "2003-03-13T21:43:18Z",
    "timestamp": 1047591798000
  },
  "page": "1694-1697",
  "source": "Crossref",
  "is-referenced-by-count": 739,
  "title": "Dispelling the Myths--Biocatalysis in Industrial Synthesis",
  "prefix": "10.1126",
  "volume": "299",
  "author": [
    {
      "given": "Hans E.",
      "family": "Schoemaker",
      "sequence": "first",
      "affiliation": [
        {
          "name": "DSM Research, Life Science Products, Post Office Box 18, 6160 MD Geleen, Netherlands."
        },
        {
          "name": "Institute of Molecular Chemistry, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS Amsterdam, Netherlands."
        }
      ]
    },
    {
      "given": "Daniel",
      "family": "Mink",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "DSM Research, Life Science Products, Post Office Box 18, 6160 MD Geleen, Netherlands."
        }
      ]
    },
    {
      "given": "Marcel G.",
      "family": "Wubbolts",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "DSM Research, Life Science Products, Post Office Box 18, 6160 MD Geleen, Netherlands."
        },
        {
          "name": "DSM Biotech, Karl-Heinz-Beckurtsstrasse 13, 52428 Ju\u0308lich, Germany."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_1_2_2",
      "doi-asserted-by": "crossref",
      "unstructured": "K. Drauz H. Waldmann Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook (Wiley-VCH Weinheim Germany ed. 2 2002).",
      "DOI": "10.1002/9783527618262"
    },
    {
      "key": "e_1_3_1_3_2",
      "doi-asserted-by": "crossref",
      "unstructured": "A. Liese K. Seelbach C. Wandrey Industrial Biotransformations (Wiley-VCH Weinheim Germany 2000).",
      "DOI": "10.1002/9783527614165"
    },
    {
      "key": "e_1_3_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35051736"
    },
    {
      "key": "e_1_3_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1367-5931(99)00055-1"
    },
    {
      "key": "e_1_3_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.10.3192"
    },
    {
      "key": "e_1_3_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bit.10396"
    },
    {
      "key": "e_1_3_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.297.5582.798"
    },
    {
      "key": "e_1_3_1_9_2",
      "unstructured": "K. Oyama in Chirality in Industry A. N. Collins G. N. Sheldrake J. Crosby Eds. (Wiley Chichester UK 1992)."
    },
    {
      "key": "e_1_3_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/op9700643"
    },
    {
      "key": "e_1_3_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1271/bbb.60.1391"
    },
    {
      "key": "e_1_3_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1039/a809538h"
    },
    {
      "key": "e_1_3_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-7799(02)01935-2"
    },
    {
      "key": "e_1_3_1_14_2",
      "doi-asserted-by": "crossref",
      "unstructured": "R. N. Patel Stereoselective Biocatalysis (Dekker New York 2000).",
      "DOI": "10.1201/9781420027242"
    },
    {
      "key": "e_1_3_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0958-1669(02)00360-9"
    },
    {
      "key": "e_1_3_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/370389a0"
    },
    {
      "key": "e_1_3_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02932909"
    },
    {
      "key": "e_1_3_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja0259842"
    },
    {
      "key": "e_1_3_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt1297-1322"
    },
    {
      "key": "e_1_3_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1367-5931(00)00180-0"
    },
    {
      "key": "e_1_3_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/AEM.66.6.2541-2547.2000"
    },
    {
      "key": "e_1_3_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0141-0229(02)00074-1"
    },
    {
      "key": "e_1_3_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0958-1669(02)00337-3"
    },
    {
      "key": "e_1_3_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1615-4169(200108)343:6/7<662::AID-ADSC662>3.0.CO;2-I"
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0958-1669(02)00302-6"
    },
    {
      "key": "e_1_3_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja983819"
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1521-3773(20010119)40:2<284::AID-ANIE284>3.0.CO;2-N"
    },
    {
      "key": "e_1_3_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/73773"
    },
    {
      "key": "e_1_3_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0040-4020(02)00668-3"
    },
    {
      "key": "e_1_3_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-7799(00)01452-9"
    },
    {
      "key": "e_1_3_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0958-1669(00)00141-5"
    },
    {
      "key": "e_1_3_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.micro.51.1.285"
    },
    {
      "key": "e_1_3_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1521-3773(20020415)41:8<1404::AID-ANIE1404>3.0.CO;2-G"
    },
    {
      "key": "e_1_3_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja9720422"
    },
    {
      "key": "e_1_3_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1074-5521(00)00035-1"
    },
    {
      "key": "e_1_3_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1039/b001538p"
    },
    {
      "key": "e_1_3_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-7799(98)01190-1"
    },
    {
      "key": "e_1_3_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.jim.7000228"
    },
    {
      "key": "e_1_3_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s002530100787"
    },
    {
      "key": "e_1_3_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bp020281"
    },
    {
      "key": "e_1_3_1_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja00080a057"
    },
    {
      "key": "e_1_3_1_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1521-3773(20010518)40:10<1945::AID-ANIE1945>3.0.CO;2-5"
    },
    {
      "key": "e_1_3_1_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MMBR.65.1.106-118.2001"
    },
    {
      "key": "e_1_3_1_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/mben.2000.0170"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.1079237",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        10
      ]
    ],
    "date-time": "2024-01-10T02:43:50Z",
    "timestamp": 1704854630000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.1079237"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        3,
        14
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "5613",
    "published-print": {
      "date-parts": [
        [
          2003,
          3,
          14
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.1079237"
  ],
  "URL": "http://dx.doi.org/10.1126/science.1079237",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2003,
        3,
        14
      ]
    ]
  }
}