DOI: 10.1126/science.1070824. Ectodermal Wnt Function as a Neural Crest Inducer

Ectodermal Wnt Function as a Neural Crest Inducer

10.1126/science.1070824

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Neural crest cells, which generate peripheral nervous system and facial skeleton, arise at the neural plate/ectodermal border via an inductive interaction between these tissues. Wnts and bone morphogenetic proteins (BMPs) play roles in neural crest induction in amphibians and zebrafish. Here, we show that, in avians, Wnt6 is localized in ectoderm and in vivo inhibition of Wnt signaling perturbs neural crest formation. Furthermore, Wnts induce neural crest from naı̈ve neural plates in vitro in a defined medium without added factors, whereas BMPs require additives. Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos.

Bibliography

GarcÄ±Ìa-Castro, M. I., Marcelle, C., & Bronner-Fraser, M. (2002). Ectodermal Wnt Function as a Neural Crest Inducer. Science, 297(5582), 848â851.

Authors 3

Martı́n I. Garcı́a-Castro (first)
Christophe Marcelle (additional)
Marianne Bronner-Fraser (additional)

References 56 Referenced 411

N. M. Le Douarin C. Kalcheim in The Neural Crest J. B. L. Bard P. W. Barlow D. L. Kirk Eds. (Cambridge University Press Cambridge ed. 2 1999). (10.1017/CBO9780511897948)
J. Rollhäuser-ter Horst Anat. Embryol. 157 113 (1979). (10.1007/BF00315644)
10.1016/0012-1606(89)90295-9
10.1242/dev.121.2.525
M. E. Dickinson M. A. Selleck A. P. McMahon M. Bronner-Fraser Development 121 2099 (July 1995). (10.1242/dev.121.7.2099)
10.1016/0092-8674(95)90276-7
10.1016/0092-8674(93)90249-P
10.1016/S0092-8674(01)80015-5
10.1146/annurev.neuro.22.1.261
10.1242/dev.125.24.4919
10.1159/000017444
10.1242/dev.122.11.3607
10.1016/0925-4773(96)00534-5
10.1242/dev.125.3.507
T. C. Dale Biochem. J. 329 ( Part 2 ) 209 (1998). (10.1042/bj3290209)
J. R. Miller Genome Biol. 3 REVIEWS3001 (2002). (10.1186/gb-2001-3-1-reviews3001)
10.1038/40146
10.1073/pnas.94.25.13713
10.1006/dbio.1997.8820
10.1242/dev.125.13.2403
10.1242/dev.128.19.3655
10.1242/dev.128.19.3665
10.1016/S0925-4773(01)00369-0
10.1101/gad.10.21.2805
10.1006/dbio.1999.9319
10.1016/S0925-4773(97)00160-3
10.1126/science.7513443
M. I. García-Castro C. Marcelle M. Bronner-Fraser data not shown.
10.1016/0045-6039(84)90049-6
10.1242/dev.115.1.355
N. R. Ramakrishna A. M. Brown Development ( Suppl ) 95 (1993). (10.1242/dev.119.Supplement.95)
10.1038/382225a0
Q. Xu P. A. D'Amore
10.1242/dev.125.23.4767
10.1038/18899
10.1002/j.1460-2075.1996.tb00830.x
10.1016/0092-8674(93)90248-O
10.1242/dev.123.1.95
10.1016/S0925-4773(97)00659-X
10.1006/dbio.1998.8927
10.1242/dev.127.6.1209
Connolly D. J., Patel K., Cooke J., Int. J. Dev. Biol. 41, 389 (1997). / Int. J. Dev. Biol. by Connolly D. J. (1997)
10.1101/gad.12.10.1438
10.1101/gad.9.17.2105
10.1101/gad.11.4.451
10.1038/375787a0
. The following probes were used: Slug [from A. Nieto (16)]; Wnts1 3a 4 5a 5b and 6 (from A. McMahon); Wnt8c (from J. Dodd); BMP-4 (from D. Wu and P. Brickell); and BMP-7 (from M. Dickinson).
Intermediate neural plates from stage 10 [as described by
10.1002/jmor.1050880104
] White Leghorn chicken embryos were dissected and cultured [as described in (5 10 11) and also based on (6–8 35)].
S 2 and S 2 -Wg cells (gift of R. Nusse) were grown [as recommended in
10.1038/368342a0
] and DMEM pen/strep control-CM and Wg-CM were collected in 1/10 original volume. Wg (54 kD) was identified by Western blot analysis.
10.1242/dev.126.1.63
A Hind III–Snc B1 fragment from mouse DnWnt1 [gift of R. Moon (23)] was cloned into a Hind III–Sma fragment of pC1 Neo (Promega Madison WI); permanently transfected 3T3 cells were selected after 3 months. Cell labeling and injections were performed [as described in (10 11)]. Expression of mRNA from DnWnt1 was detected after injection. Wnt1-cell lines were kindly provided by N. Brown and A. Kiespert.
Supported by NIH grant number NS36585. We thank C. Baker A. Groves and A. Knecht for helpful comments on this work.

Dates

Type	When
Created	3 years, 10 months ago (Oct. 21, 2021, 10:35 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:57 p.m.)
Indexed	5 days, 10 hours ago (Aug. 21, 2025, 1:45 p.m.)
Issued	23 years ago (Aug. 2, 2002)
Published	23 years ago (Aug. 2, 2002)
Published Print	23 years ago (Aug. 2, 2002)

Funders 0

None

BibTeX

@article{Garc_a_Castro_2002, title={Ectodermal Wnt Function as a Neural Crest Inducer}, volume={297}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1070824}, DOI={10.1126/science.1070824}, number={5582}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Garcı́a-Castro, Martı́n I. and Marcelle, Christophe and Bronner-Fraser, Marianne}, year={2002}, month=aug, pages={848–851} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T17:45:51Z",
    "timestamp": 1755798351648
  },
  "reference-count": 56,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5582",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        8,
        2
      ]
    ]
  },
  "abstract": "<jats:p>Neural crest cells, which generate peripheral nervous system and facial skeleton, arise at the neural plate/ectodermal border via an inductive interaction between these tissues. Wnts and bone morphogenetic proteins (BMPs) play roles in neural crest induction in amphibians and zebrafish. Here, we show that, in avians, Wnt6 is localized in ectoderm and in vivo inhibition of Wnt signaling perturbs neural crest formation. Furthermore, Wnts induce neural crest from na\u0131\u0308ve neural plates in vitro in a defined medium without added factors, whereas BMPs require additives. Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos.</jats:p>",
  "DOI": "10.1126/science.1070824",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        10,
        21
      ]
    ],
    "date-time": "2021-10-21T14:35:35Z",
    "timestamp": 1634826935000
  },
  "page": "848-851",
  "source": "Crossref",
  "is-referenced-by-count": 411,
  "title": "Ectodermal Wnt Function as a Neural Crest Inducer",
  "prefix": "10.1126",
  "volume": "297",
  "author": [
    {
      "given": "Mart\u0131\u0301n I.",
      "family": "Garc\u0131\u0301a-Castro",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Division of Biology 139-74, California Institute of Technology, Wilson and California, Pasadena, CA 91125, USA."
        }
      ]
    },
    {
      "given": "Christophe",
      "family": "Marcelle",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Division of Biology 139-74, California Institute of Technology, Wilson and California, Pasadena, CA 91125, USA."
        }
      ]
    },
    {
      "given": "Marianne",
      "family": "Bronner-Fraser",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Division of Biology 139-74, California Institute of Technology, Wilson and California, Pasadena, CA 91125, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_1_2_2",
      "doi-asserted-by": "crossref",
      "unstructured": "N. M. Le Douarin C. Kalcheim in The Neural Crest J. B. L. Bard P. W. Barlow D. L. Kirk Eds. (Cambridge University Press Cambridge ed. 2 1999).",
      "DOI": "10.1017/CBO9780511897948"
    },
    {
      "key": "e_1_3_1_3_2",
      "doi-asserted-by": "crossref",
      "unstructured": "J. Rollh\u00e4user-ter Horst Anat. Embryol. 157 113 (1979).",
      "DOI": "10.1007/BF00315644"
    },
    {
      "key": "e_1_3_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(89)90295-9"
    },
    {
      "key": "e_1_3_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.121.2.525"
    },
    {
      "key": "e_1_3_1_6_2",
      "doi-asserted-by": "crossref",
      "unstructured": "M. E. Dickinson M. A. Selleck A. P. McMahon M. Bronner-Fraser Development 121 2099 (July 1995).",
      "DOI": "10.1242/dev.121.7.2099"
    },
    {
      "key": "e_1_3_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90276-7"
    },
    {
      "key": "e_1_3_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90249-P"
    },
    {
      "key": "e_1_3_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)80015-5"
    },
    {
      "key": "e_1_3_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.neuro.22.1.261"
    },
    {
      "key": "e_1_3_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.125.24.4919"
    },
    {
      "key": "e_1_3_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000017444"
    },
    {
      "key": "e_1_3_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.122.11.3607"
    },
    {
      "key": "e_1_3_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0925-4773(96)00534-5"
    },
    {
      "key": "e_1_3_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.125.3.507"
    },
    {
      "key": "e_1_3_1_16_2",
      "doi-asserted-by": "crossref",
      "unstructured": "T. C. Dale Biochem. J. 329 ( Part 2 ) 209 (1998).",
      "DOI": "10.1042/bj3290209"
    },
    {
      "key": "e_1_3_1_17_2",
      "doi-asserted-by": "crossref",
      "unstructured": "J. R. Miller Genome Biol. 3 REVIEWS3001 (2002).",
      "DOI": "10.1186/gb-2001-3-1-reviews3001"
    },
    {
      "key": "e_1_3_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/40146"
    },
    {
      "key": "e_1_3_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.25.13713"
    },
    {
      "key": "e_1_3_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1997.8820"
    },
    {
      "key": "e_1_3_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.125.13.2403"
    },
    {
      "key": "e_1_3_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.128.19.3655"
    },
    {
      "key": "e_1_3_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.128.19.3665"
    },
    {
      "key": "e_1_3_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0925-4773(01)00369-0"
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.21.2805"
    },
    {
      "key": "e_1_3_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1999.9319"
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0925-4773(97)00160-3"
    },
    {
      "key": "e_1_3_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7513443"
    },
    {
      "key": "e_1_3_1_29_2",
      "unstructured": "M. I. Garc\u00eda-Castro C. Marcelle M. Bronner-Fraser data not shown."
    },
    {
      "key": "e_1_3_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0045-6039(84)90049-6"
    },
    {
      "key": "e_1_3_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.115.1.355"
    },
    {
      "key": "e_1_3_1_32_2",
      "doi-asserted-by": "crossref",
      "unstructured": "N. R. Ramakrishna A. M. Brown Development ( Suppl ) 95 (1993).",
      "DOI": "10.1242/dev.119.Supplement.95"
    },
    {
      "key": "e_1_3_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/382225a0"
    },
    {
      "key": "e_1_3_1_34_2",
      "unstructured": "Q. Xu P. A. D'Amore"
    },
    {
      "key": "e_1_3_1_34_3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.125.23.4767"
    },
    {
      "key": "e_1_3_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/18899"
    },
    {
      "key": "e_1_3_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00830.x"
    },
    {
      "key": "e_1_3_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90248-O"
    },
    {
      "key": "e_1_3_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.123.1.95"
    },
    {
      "key": "e_1_3_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0925-4773(97)00659-X"
    },
    {
      "key": "e_1_3_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/dbio.1998.8927"
    },
    {
      "key": "e_1_3_1_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.127.6.1209"
    },
    {
      "key": "e_1_3_1_42_2",
      "first-page": "389",
      "volume": "41",
      "author": "Connolly D. J.",
      "year": "1997",
      "unstructured": "Connolly D. J., Patel K., Cooke J., Int. J. Dev. Biol. 41, 389 (1997).",
      "journal-title": "Int. J. Dev. Biol."
    },
    {
      "key": "e_1_3_1_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.10.1438"
    },
    {
      "key": "e_1_3_1_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.17.2105"
    },
    {
      "key": "e_1_3_1_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.4.451"
    },
    {
      "key": "e_1_3_1_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/375787a0"
    },
    {
      "key": "e_1_3_1_46_3",
      "unstructured": ". The following probes were used: Slug [from A. Nieto (16)]; Wnts1 3a 4 5a 5b and 6 (from A. McMahon); Wnt8c (from J. Dodd); BMP-4 (from D. Wu and P. Brickell); and BMP-7 (from M. Dickinson)."
    },
    {
      "key": "e_1_3_1_47_2",
      "unstructured": "Intermediate neural plates from stage 10 [as described by"
    },
    {
      "key": "e_1_3_1_47_3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jmor.1050880104"
    },
    {
      "key": "e_1_3_1_47_4",
      "unstructured": "] White Leghorn chicken embryos were dissected and cultured [as described in (5 10 11) and also based on (6\u20138 35)]."
    },
    {
      "key": "e_1_3_1_48_2",
      "unstructured": "S 2 and S 2 -Wg cells (gift of R. Nusse) were grown [as recommended in"
    },
    {
      "key": "e_1_3_1_48_3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/368342a0"
    },
    {
      "key": "e_1_3_1_48_4",
      "unstructured": "] and DMEM pen/strep control-CM and Wg-CM were collected in 1/10 original volume. Wg (54 kD) was identified by Western blot analysis."
    },
    {
      "key": "e_1_3_1_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.126.1.63"
    },
    {
      "key": "e_1_3_1_50_2",
      "unstructured": "A Hind III\u2013Snc B1 fragment from mouse DnWnt1 [gift of R. Moon (23)] was cloned into a Hind III\u2013Sma fragment of pC1 Neo (Promega Madison WI); permanently transfected 3T3 cells were selected after 3 months. Cell labeling and injections were performed [as described in (10 11)]. Expression of mRNA from DnWnt1 was detected after injection. Wnt1-cell lines were kindly provided by N. Brown and A. Kiespert."
    },
    {
      "key": "e_1_3_1_51_2",
      "unstructured": "Supported by NIH grant number NS36585. We thank C. Baker A. Groves and A. Knecht for helpful comments on this work."
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.1070824",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        10
      ]
    ],
    "date-time": "2024-01-10T02:57:29Z",
    "timestamp": 1704855449000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.1070824"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        8,
        2
      ]
    ]
  },
  "references-count": 56,
  "journal-issue": {
    "issue": "5582",
    "published-print": {
      "date-parts": [
        [
          2002,
          8,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.1070824"
  ],
  "URL": "http://dx.doi.org/10.1126/science.1070824",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2002,
        8,
        2
      ]
    ]
  }
}