DOI: 10.1126/science.1069861. A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G <sub>0</sub> Cells

A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 Cells

10.1126/science.1069861

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1γ, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G 0 cells rather than in G 1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.

Bibliography

Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D. M., & Nakatani, Y. (2002). A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 Cells. Science, 296(5570), 1132â1136.

Authors 5

Hidesato Ogawa (first)
Kei-ichiro Ishiguro (additional)
Stefan Gaubatz (additional)
David M. Livingston (additional)
Yoshihiro Nakatani (additional)

References 24 Referenced 630

10.1101/gad.12.15.2245
Muller H., Helin K., Biochim. Biophys. Acta 1470, M1 (2000). / Biochim. Biophys. Acta by Muller H. (2000)
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J. M. Trimarchi et al. Proc. Natl. Acad. Sci. U.S.A. 95 2850 (1998). (10.1073/pnas.95.6.2850)
Materials and methods are available as supporting online material.
H. Ogawa Y. Nakatani unpublished observations.
10.1093/emboj/18.24.7019
10.1002/bies.1114
GenBank accession number .
10.1074/jbc.M101914200
10.1007/s00335-001-3029-3
M. Lachner D. O'Carroll
10.1038/35065132
A. J. Bannister et al. Nature 410 120 (2001). (10.1038/35065138)
10.1002/(SICI)1521-1878(200002)22:2<124::AID-BIES4>3.0.CO;2-E
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We would like to thank colleagues in the Nakatani and Livingston labs for discussions and reagents; B. Eisenman M. van Lohuizen and G. Cavalli for useful comments; P. Hurlin J. Lees and M. Vidal for antibodies; members of the DFCI Molecular Core Facility and Taplin Biological Mass Spectrometry Facility for their assistance. This work was supported in part by grants from Claudia Adams Barr Program (Y.N.) Human Frontier Science Program (Y.N.) and the National Cancer Institute (D.M.L.). H.O. is supported by a fellowship from Japan Science and Technology. Supporting Online Material (www.sciencemag.org/cgi/content/full/296/5570/1132/DC1) Materials and Methods References

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:54 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 9:36 p.m.)
Indexed	16 hours, 43 minutes ago (Aug. 29, 2025, 6:08 a.m.)
Issued	23 years, 3 months ago (May 10, 2002)
Published	23 years, 3 months ago (May 10, 2002)
Published Print	23 years, 3 months ago (May 10, 2002)

Funders 0

None

BibTeX

@article{Ogawa_2002, title={A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 Cells}, volume={296}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1069861}, DOI={10.1126/science.1069861}, number={5570}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ogawa, Hidesato and Ishiguro, Kei-ichiro and Gaubatz, Stefan and Livingston, David M. and Nakatani, Yoshihiro}, year={2002}, month=may, pages={1132–1136} }

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