DOI: 10.1126/science.1067765. Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway

Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway

10.1126/science.1067765

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.

Bibliography

Kurz, T., Pintard, L., Willis, J. H., Hamill, D. R., GoÌnczy, P., Peter, M., & Bowerman, B. (2002). Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway. Science, 295(5558), 1294â1298.

Authors 7

Thimo Kurz (first)
Lionel Pintard (additional)
John H. Willis (additional)
Danielle R. Hamill (additional)
Pierre Gönczy (additional)
Matthias Peter (additional)
Bruce Bowerman (additional)

References 36 Referenced 165

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10.1016/S0960-9822(00)00703-X
We thank S. Schneider for phylogenetic analysis of RFL-1; P. Mains Y. Bobinnec S. Strome and A. Severson for providing antibodies; J. Austin and C. Malone for providing GFP lines; Y. Kohara (National Institute of Genetics Mishima Japan) and E. Kipreos for providing cDNA clones; the C. elegans Genetics Center funded by the NIH National Center for Research Resources for providing strains; C. Doe A. Severson and D. Williams for helpful discussions and comments on the manuscript; and V. Hemleben U. Zentgraf (University of Tübingen Germany) and U. Brügge (Ludwig-Wilhelm-Gymnasium Rastatt Germany) for their help and teaching. T.K. was supported by a predoctoral fellowship from the American Heart Association; L.P. by a long-term fellowship from the Federation of European Biochemical Societies; J.H.W. by an NIH Molecular Biology Training Grant; D.R.H. by a postdoctoral fellowship from the American Cancer Society; P.G. and M.P. by the Swiss National Science Foundation; and B.B. was supported by the NIH (R01GM58017).

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 5:25 p.m.)
Indexed	2 months ago (July 2, 2025, 2:31 p.m.)
Issued	23 years, 6 months ago (Feb. 15, 2002)
Published	23 years, 6 months ago (Feb. 15, 2002)
Published Print	23 years, 6 months ago (Feb. 15, 2002)

Funders 0

None

BibTeX

@article{Kurz_2002, title={Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway}, volume={295}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1067765}, DOI={10.1126/science.1067765}, number={5558}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kurz, Thimo and Pintard, Lionel and Willis, John H. and Hamill, Danielle R. and Gönczy, Pierre and Peter, Matthias and Bowerman, Bruce}, year={2002}, month=feb, pages={1294–1298} }

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