Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
10.1126/science.1065810
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

In Saccharomyces cerevisiae , more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization ( BNI1 , ARP2 , ARC40 , BIM1 ), DNA synthesis and repair ( SGS1 , RAD27 ), or uncharacterized functions ( BBC1 , NBP2 ) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

Bibliography

Tong, A. H. Y., Evangelista, M., Parsons, A. B., Xu, H., Bader, G. D., Pagé, N., Robinson, M., Raghibizadeh, S., Hogue, C. W. V., Bussey, H., Andrews, B., Tyers, M., & Boone, C. (2001). Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants. Science, 294(5550), 2364–2368.

Authors 13
  1. Amy Hin Yan Tong (first)
  2. Marie Evangelista (additional)
  3. Ainslie B. Parsons (additional)
  4. Hong Xu (additional)
  5. Gary D. Bader (additional)
  6. Nicholas Pagé (additional)
  7. Mark Robinson (additional)
  8. Sasan Raghibizadeh (additional)
  9. Christopher W. V. Hogue (additional)
  10. Howard Bussey (additional)
  11. Brenda Andrews (additional)
  12. Mike Tyers (additional)
  13. Charles Boone (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:47 a.m.)
Deposited 1 year, 7 months ago (Jan. 9, 2024, 6:02 p.m.)
Indexed 1 week, 3 days ago (Aug. 20, 2025, 8:43 a.m.)
Issued 23 years, 8 months ago (Dec. 14, 2001)
Published 23 years, 8 months ago (Dec. 14, 2001)
Published Print 23 years, 8 months ago (Dec. 14, 2001)
Funders 0

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@article{Tong_2001, title={Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants}, volume={294}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1065810}, DOI={10.1126/science.1065810}, number={5550}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tong, Amy Hin Yan and Evangelista, Marie and Parsons, Ainslie B. and Xu, Hong and Bader, Gary D. and Pagé, Nicholas and Robinson, Mark and Raghibizadeh, Sasan and Hogue, Christopher W. V. and Bussey, Howard and Andrews, Brenda and Tyers, Mike and Boone, Charles}, year={2001}, month=dec, pages={2364–2368} }