DOI: 10.1126/science.1064027. Requirement of Heterochromatin for Cohesion at Centromeres

Requirement of Heterochromatin for Cohesion at Centromeres

10.1126/science.1064027

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Centromeres are heterochromatic in many organisms, but the mitotic function of this silent chromatin remains unknown. During cell division, newly replicated sister chromatids must cohere until anaphase when Scc1/Rad21–mediated cohesion is destroyed. In metazoans, chromosome arm cohesins dissociate during prophase, leaving centromeres as the only linkage before anaphase. It is not known what distinguishes centromere cohesion from arm cohesion. Fission yeast Swi6 (a Heterochromatin protein 1 counterpart) is a component of silent heterochromatin. Here we show that this heterochromatin is specifically required for cohesion between sister centromeres. Swi6 is required for association of Rad21-cohesin with centromeres but not along chromosome arms and, thus, acts to distinguish centromere from arm cohesion. Therefore, one function of centromeric heterochromatin is to attract cohesin, thereby ensuring sister centromere cohesion and proper chromosome segregation.

Bibliography

Bernard, P., Maure, J.-F., Partridge, J. F., Genier, S., Javerzat, J.-P., & Allshire, R. C. (2001). Requirement of Heterochromatin for Cohesion at Centromeres. Science, 294(5551), 2539â2542.

Authors 6

Pascal Bernard (first)
Jean-François Maure (additional)
Janet F. Partridge (additional)
Sylvie Genier (additional)
Jean-Paul Javerzat (additional)
Robin C. Allshire (additional)

References 31 Referenced 510

10.1016/S0962-8924(00)01821-3
10.1038/21831
10.1016/S0092-8674(00)81518-4
10.1083/jcb.151.5.1047
10.1101/gad.832000
10.1016/S0092-8674(00)00132-X
10.1016/S0960-9822(00)00806-X
10.1016/S0168-9525(97)01298-5
10.1093/genetics/131.3.683
10.1126/science.273.5271.118
10.1101/gad.14.7.783
10.1101/gad.9.2.218
A. L. Pidoux R. C. Allshire unpublished data.
K. Ekwall et al. Science 269 1429 (1995). (10.1126/science.7660126)
10.1016/S0092-8674(00)80492-4
10.1242/jcs.113.23.4177
10.1038/35053103
10.1083/jcb.143.7.1775
Supplemental material is available at Science Online at www.sciencemag.org/cgi/content/full/1064027/DC1.
H. Yamada K. Kumada M. Yanagida J. Cell Sci. 110 (15) 1793 (1997). (10.1242/jcs.110.15.1793)
J. Nakayama et al. Science 292 110 (2001). (10.1126/science.1060118)
10.1038/35020506
10.1038/35065138
10.1038/35055010
10.1083/jcb.153.3.517
10.1038/35019529
E. T. O'Toole
10.1091/mbc.10.6.2017
10.1083/jcb.120.1.141
Cosmids SPA c56F8 {37 kb} c493 [37 kb] c102 [36 kb] c147 [37.7 kb] c1480 [36.8 kb] c1694 [44 kb] (including c8C9) c1556 [36.6 kb] c323 [39.3 kb] c23A1 {36 kb} c1F7 {34 kb} c23D3 {42 kb} and c27D7 {36 kb} were used as FISH probes. These were identified at www.sanger.ac.uk/Projects/S_pombe/ and obtained from the Sanger Centre UK. Square brackets indicate physical size whereas curly brackets indicate the size of the sequenced portion of the cosmid. Cosmids were prepared and utilized for FISH as previously described (14).
We thank Allshire lab members for helpful input M. Yanagida for cut9-665 H. Ikeda for rad21-K1 and K. Gull for TAT1. Allshire lab centromere research is supported by MRC core funding. P.B. is supported by a Wellcome Trust Travelling Research Fellowship. Research in the Javerzat lab is supported by the Centre National de la Recherche Scientifique and l'Association pour la Recherche sur le Cancer and J.-F.M. by a fellowship from the Ministère de la Recherche et de l'Enseignement Supérieur.

Dates

Type	When
Created	23 years ago (July 27, 2002, 5:47 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 6:12 p.m.)
Indexed	1 month ago (July 27, 2025, 3:48 a.m.)
Issued	23 years, 8 months ago (Dec. 21, 2001)
Published	23 years, 8 months ago (Dec. 21, 2001)
Published Print	23 years, 8 months ago (Dec. 21, 2001)

Funders 0

None

BibTeX

@article{Bernard_2001, title={Requirement of Heterochromatin for Cohesion at Centromeres}, volume={294}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1064027}, DOI={10.1126/science.1064027}, number={5551}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Bernard, Pascal and Maure, Jean-François and Partridge, Janet F. and Genier, Sylvie and Javerzat, Jean-Paul and Allshire, Robin C.}, year={2001}, month=dec, pages={2539–2542} }

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