An Autoinhibitory Mechanism for Nonsyntaxin SNARE Proteins Revealed by the Structure of Ykt6p
10.1126/science.1062950
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Ykt6p is a nonsyntaxin SNARE implicated in multiple intracellular membrane trafficking steps. Here we present the structure of the NH 2 -terminal domain of Ykt6p (Ykt6pN, residues 1 to 140). The structure of Ykt6pN differed entirely from that of syntaxin and resembled the overall fold of the actin regulatory protein, profilin. Like some syntaxins, Ykt6p adopted a folded back conformation in which Ykt6pN bound to its COOH-terminal core domain. The NH 2 -terminal domain plays an important biological role in the function of Ykt6p, which in vitro studies revealed to include influencing the kinetics and proper assembly of SNARE complexes.

Bibliography

Tochio, H., Tsui, M. M. K., Banfield, D. K., & Zhang, M. (2001). An Autoinhibitory Mechanism for Nonsyntaxin SNARE Proteins Revealed by the Structure of Ykt6p. Science, 293(5530), 698–702.

Authors 4
  1. Hidehito Tochio (first)
  2. Marco M. K. Tsui (additional)
  3. David K. Banfield (additional)
  4. Mingjie Zhang (additional)
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  40. We thank F. Hung and Q. Zhang for assistance in protein purification and gel-filtration experiments respectively and C. Rock for comments on the manuscript. This work was supported by grants from the Research Grant Council of Hong Kong to D.K.B and M.Z. and by a grant from the Human Frontiers Science Program Organization to M.Z. The NMR spectrometer used in this work was purchased with funds donated to the Biotechnology Research Institute by the Hong Kong Jockey Club. The coordinates of the structure of Ykt6pN have been deposited in the Protein Data Bank (accession code 1h8m).
Dates
Type When
Created 19 years, 5 months ago (March 28, 2006, 1:37 p.m.)
Deposited 1 year, 7 months ago (Jan. 9, 2024, 5:11 p.m.)
Indexed 2 weeks, 1 day ago (Aug. 20, 2025, 8:47 a.m.)
Issued 24 years, 1 month ago (July 27, 2001)
Published 24 years, 1 month ago (July 27, 2001)
Published Print 24 years, 1 month ago (July 27, 2001)
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@article{Tochio_2001, title={An Autoinhibitory Mechanism for Nonsyntaxin SNARE Proteins Revealed by the Structure of Ykt6p}, volume={293}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1062950}, DOI={10.1126/science.1062950}, number={5530}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tochio, Hidehito and Tsui, Marco M. K. and Banfield, David K. and Zhang, Mingjie}, year={2001}, month=jul, pages={698–702} }