DOI: 10.1126/science.1057176. Requirement for the SLP-76 Adaptor GADS in T Cell Development

Requirement for the SLP-76 Adaptor GADS in T Cell Development

10.1126/science.1057176

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS – CD4 – CD8 – thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS – thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS – thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.

Bibliography

Yoder, J., Pham, C., Iizuka, Y.-M., Kanagawa, O., Liu, S. K., McGlade, J., & Cheng, A. M. (2001). Requirement for the SLP-76 Adaptor GADS in T Cell Development. Science, 291(5510), 1987â1991.

Authors 7

Jeff Yoder (first)
Christine Pham (additional)
Yoshie-Matsubayashi Iizuka (additional)
Osami Kanagawa (additional)
Stanley K. Liu (additional)
Jane McGlade (additional)
Alec M. Cheng (additional)

References 29 Referenced 122

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Thymic DN cells were purified by Dynal magnetic beads. Purified samples were stained with antibody to CD25-FITC washed and fixed in 70% ethanol. Cells were washed and treated with propidium iodide (10 mg/ml) and ribonuclease A (250 mg/ml). Cells were gated for singlets and CD25 + and DNA content was determined by FL2-H. 30 . We thank A. C. Chan and laboratory for many suggestions technical advice and generous gifts of reagents and the Dr. Hector Molina laboratory for help and support. Supported by a research startup fund provided by the Department of Medicine and the Department of Pathology and Immunology at the Washington University School of Medicine.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:48 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 6:06 p.m.)
Indexed	2 months ago (July 2, 2025, 6:01 p.m.)
Issued	24 years, 5 months ago (March 9, 2001)
Published	24 years, 5 months ago (March 9, 2001)
Published Print	24 years, 5 months ago (March 9, 2001)

Funders 0

None

BibTeX

@article{Yoder_2001, title={Requirement for the SLP-76 Adaptor GADS in T Cell Development}, volume={291}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1057176}, DOI={10.1126/science.1057176}, number={5510}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yoder, Jeff and Pham, Christine and Iizuka, Yoshie-Matsubayashi and Kanagawa, Osami and Liu, Stanley K. and McGlade, Jane and Cheng, Alec M.}, year={2001}, month=mar, pages={1987–1991} }

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  "abstract": "<jats:p>\n            GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS\n            <jats:sup>\u2013</jats:sup>\n            CD4\n            <jats:sup>\u2013</jats:sup>\n            CD8\n            <jats:sup>\u2013</jats:sup>\n            thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS\n            <jats:sup>\u2013</jats:sup>\n            thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS\n            <jats:sup>\u2013</jats:sup>\n            thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.\n          </jats:p>",
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