DOI: 10.1124/pr.58.1.8. Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery

Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery

10.1124/pr.58.1.8

Crossref journal-article

Elsevier BV

Pharmacological Reviews (78)

Bibliography

Khalil, I. A., Kogure, K., Akita, H., & Harashima, H. (2006). Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery. Pharmacological Reviews, 58(1), 32â45.

Authors 4

Ikramy A. Khalil (first)
Kentaro Kogure (additional)
Hidetaka Akita (additional)
Hideyoshi Harashima (additional)

References 127 Referenced 1,064

10.1016/j.ymthe.2004.01.005 / Mol Ther / Quantitative three-dimensional analysis of the intracellular trafficking of plasmid DNA transfected by a nonviral gene delivery system using confocal laser scanning microscopy by Akita (2004)
10.1016/S0952-7915(96)80102-6 / Curr Opin Immunol / Mechanisms of phagocytosis by Allen (1996)
10.1016/0014-5793(87)80506-9 / FEBS Lett / Large unilamellar liposomes with low uptake into the reticuloendothelial system by Allen (1987)
10.1016/S0003-9861(02)00725-7 / Arch Biochem Biophys / Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization by Almofti (2003)
10.1078/1438-4221-00157 / Int J Med Microbiol / Origin, originality, functions, subversions and molecular signalling of macropinocytosis by Amyere (2002)
10.1126/science.1310359 / Science (Wash DC) / Potocytosis: sequestration and transport of small molecules by caveolae by Anderson (1992)
10.1042/bj2960297 / Biochem J / Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses by Arcaro (1993)
10.1016/S0960-9822(00)00859-9 / Curr Biol / Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton by Ayscough (2000)
10.1016/S0169-409X(99)00034-4 / Adv Drug Deliv Rev / Biological barriers to cellular delivery of lipid-based DNA carriers by Bally (1999)
{'key': '10.1124/pr.58.1.8_REF10', 'first-page': '1016', 'article-title': 'Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O', 'volume': '15', 'author': 'Barry', 'year': '1993', 'journal-title': 'Biotechniques'} / Biotechniques / Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O by Barry (1993)
10.1073/pnas.86.18.6982 / Proc Natl Acad Sci USA / Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA by Behr (1989)
10.1093/nar/21.15.3567 / Nucleic Acids Res / Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy by Bergan (1993)
10.1073/pnas.92.16.7297 / Proc Natl Acad Sci USA / A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine by Boussif (1995)
10.1146/annurev.cellbio.17.1.517 / Annu Rev Cell Dev Biol / Biological basket weaving: formation and function of clathrin-coated vesicles by Brodsky (2001)
10.1016/j.addr.2004.12.001 / Adv Drug Deliv Rev / Tat peptide-mediated cellular delivery: back to basics by Brooks (2005)
10.1002/mabi.200500018 / Macromol Biosci / Folate receptor-mediated gene delivery using folate-poly(ethylene glycol)-poly(l-lysine) conjugate by Cho (2005)
10.1002/jps.10437 / J Pharm Sci / Biophysical characterization of PEI/DNA complexes by Choosakoonkriang (2003)
10.1038/nature01451 / Nature (Lond) / Regulated portals of entry into the cell by Conner (2003)
10.1073/pnas.87.11.4033 / Proc Natl Acad Sci USA / Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels by Cotten (1990)
10.1016/0009-3084(86)90067-8 / Chem Phys Lipids / Lipid polymorphism and the roles of lipids in membranes by Cullis (1986)
10.1083/jcb.127.4.915 / J Cell Biol / Induction of mutant dynamin specifically blocks endocytic coated vesicle formation by Damke (1994)
10.1016/0006-2952(74)90174-9 / Biochem Pharmacol / Commentary: lysosomotropic agents by de Duve (1974)
10.1074/jbc.271.30.18188 / J Biol Chem / Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent by Derossi (1996)
10.1016/S0169-409X(00)00082-X / Adv Drug Deliv Rev / Novel non-endocytic delivery of antisense oligonucleotides by Dokka (2000)
10.1242/jeb.200.1.1 / J Exp Biol / Bafilomycins and concanamycins as inhibitors of V-ATPases and P-ATPases by Drose (1997)
10.1083/jcb.107.4.1279 / J Cell Biol / The effects of variations in the number and sequence of targeting signals on nuclear uptake by Dworetzky (1988)
10.1016/S0005-2736(96)00242-8 / Biochim Biophys Acta / Biophysical characterization of cationic lipid: DNA complexes by Eastman (1997)
10.1016/0005-2736(95)80016-9 / Biochim Biophys Acta / The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer by Farhood (1995)
10.1073/pnas.84.21.7413 / Proc Natl Acad Sci USA / Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure by Felgner (1987)
10.1111/j.1749-6632.1995.tb44738.x / Ann NY Acad Sci / Improved cationic lipid formulations for in vivo gene therapy by Felgner (1995)
10.1016/S1525-0016(03)00122-9 / Mol Ther / Caveolae-mediated internalization of extracellular HIV-1 tat fusion proteins visualized in real time by Ferrari (2003)
10.1074/jbc.M303045200 / J Biol Chem / Cell membrane lipid rafts mediate caveolar endocytosis of HIV-1 Tat fusion proteins by Fittipaldi (2003)
10.1016/0005-2736(95)00219-7 / Biochim Biophys Acta / Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes by Friend (1996)
10.1034/j.1600-0854.2000.010208.x / Traffic / Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis in mammalian cells by Fujimoto (2000)
10.1021/bc015508l / Bioconjug Chem / Stearylated arginine-rich peptides: a new class of transfection systems by Futaki (2001)
10.1146/annurev.cb.01.110185.000245 / Annu Rev Cell Biol / Receptor-mediated endocytosis: concepts emerging from the LDL receptor system by Goldstein (1985)
10.1016/j.ymthe.2004.05.023 / Mol Ther / Macropinocytosis of polyplexes and recycling of plasmid via the clathrindependent pathway impair the transfection efficiency of human hepatocarcinoma cells by Goncalves (2004)
10.1016/j.addr.2004.10.007 / Adv Drug Deliv Rev / Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides by Gupta (2005)
10.1016/S1471-4906(01)02161-5 / Trends Immunol / Caveolae and caveolin in immune cells: distribution and functions by Harris (2002)
10.1083/jcb.108.2.389 / J Cell Biol / Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation by Heuser (1989)
10.1083/jcb.124.5.689 / J Cell Biol / The coated pit and macropinocytic pathways serve distinct endosome populations by Hewlett (1994)
10.1016/0925-4439(91)90016-3 / Biochim Biophys Acta / Liposomes for the transformation of eukaryotic cells by Hug (1991)
10.1139/o96-068 / Biochem Cell Biol / Spermidine-condensed DNA and cone-shaped lipids improve delivery and expression of exogenous DNA transfer by liposomes by Ibanez (1996)
10.1152/physrev.1996.76.3.651 / Physiol Rev / Regulation of protein transport to the nucleus: central role of phosphorylation by Jans (1996)
10.1091/mbc.4.12.1251 / Mol Biol Cell / Endosome acidification and receptor trafficking: bafilomycin A1 slows receptor externalization by a mechanism involving the receptor’s internalization motif by Johnson (1993)
10.1021/bi035802w / Biochemistry / Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system by Kakudo (2004)
10.1016/S1359-6446(03)02889-7 / Drug Discov Today / Pharmacokinetic and pharmacodynamic considerations in gene therapy by Kamiya (2003)
10.1016/S0006-291X(02)02485-3 / Biochem Biophys Res Commun / Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes by Kamiya (2002)
10.1016/j.jconrel.2004.10.018 / J Control Release / Cationic TAT peptide transduction domain enters cells by macropinocytosis by Kaplan (2005)
{'key': '10.1124/pr.58.1.8_REF50', 'first-page': '405', 'article-title': 'Effect of cationic charge on receptor-mediated transfection using mannosylated cationic liposome/plasmid DNA complexes following the intravenous administration in mice', 'volume': '59', 'author': 'Kawakami', 'year': '2004', 'journal-title': 'Pharmazie'} / Pharmazie / Effect of cationic charge on receptor-mediated transfection using mannosylated cationic liposome/plasmid DNA complexes following the intravenous administration in mice by Kawakami (2004)
10.1038/sj.gt.3302128 / Gene Ther / Mechanism of improved gene transfer by the N-terminal stearylation of octaarginine: enhanced cellular association by hydrophobic core formation by Khalil (2004)
Khalil IA, Kogure K, Futaki S, and Harashima H (2006) High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression. J Biol Chem, in press. (10.1074/jbc.M503202200)
10.1002/jgm.173 / J Gene Med / Polyethylenimine-mediated gene delivery: a mechanistic study by Kichler (2001)
10.1016/S0169-409X(97)00132-4 / Adv Drug Deliv Rev / Identification of proteins mediating clearance of liposomes using a liver perfusion system by Kiwada (1998)
10.1016/S0006-2952(98)00098-7 / Biochem Pharmacol / Effects of polyethyleneimine on endocytosis and lysosome stability by Klemm (1998)
10.1038/353043a0 / Nature (Lond) / Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents by Kleuss (1991)
10.1016/j.jconrel.2004.04.024 / J Control Release / Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method by Kogure (2004)
10.1002/jgm.558 / J Gene Med / A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin by Kopatz (2004)
{'key': '10.1124/pr.58.1.8_REF59', 'first-page': '1010', 'article-title': 'An electron microscopy study into the mechanism of gene transfer with lipopolyamines', 'volume': '3', 'author': 'Labat-Moleur', 'year': '1996', 'journal-title': 'Gene Ther'} / Gene Ther / An electron microscopy study into the mechanism of gene transfer with lipopolyamines by Labat-Moleur (1996)
10.1016/0955-0674(95)80015-8 / Curr Opin Cell Biol / The emergence of clathrin-independent pinocytic pathways by Lamaze (1995)
10.1016/0092-8674(83)90356-2 / Cell / Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblasts by Larkin (1983)
10.1016/0958-1669(94)90085-X / Curr Opin Biotechnol / Non-viral gene therapy by Ledley (1994)
10.1074/jbc.271.14.8481 / J Biol Chem / Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer by Lee (1996)
10.1073/pnas.88.7.2702 / Proc Natl Acad Sci USA / Intracellular distribution of microinjected antisense oligonucleotides by Leonetti (1991)
10.1038/sj.gt.3301110 / Gene Ther / Nonviral gene therapy: promises and challenges by Li (2000)
10.1016/j.addr.2003.10.041 / Adv Drug Deliv Rev / GALA: a designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery by Li (2004)
10.1038/nbt0297-167 / Nat Biotechnol / Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery by Liu (1997)
10.1016/S1525-0016(03)00135-7 / Mol Ther / Cell surface adherence and endocytosis of protein transduction domains by Lundberg (2003)
10.1074/jbc.272.2.1117 / J Biol Chem / Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells by Matsui (1997)
10.1016/S0169-409X(01)00138-7 / Adv Drug Deliv Rev / The role of caveolae and caveolin in vesicle-dependent and vesicle-independent trafficking by Matveev (2001)
10.1038/nrm1315 / Nat Rev Mol Cell Biol / Endocytic recycling by Maxfield (2004)
10.1002/jgm.409 / J Gene Med / Adenovirus endocytosis by Meier (2003)
10.1016/0955-0674(95)80084-0 / Curr Opin Cell Biol / Mechanisms of nuclear protein import by Melchior (1995)
10.1146/annurev.bi.55.070186.003311 / Annu Rev Biochem / Acidification of the endocytic and exocytic pathways by Mellman (1986)
10.1016/0005-2736(95)00163-W / Biochim Biophys Acta / Membrane permeabilization by α-helical peptides: a flow cytometry study by Midoux (1995)
10.1073/pnas.93.22.12349 / Proc Natl Acad Sci USA / Evidence for the role of proteoglycans in cation-mediated gene transfer by Mislick (1996)
10.1016/0304-4157(90)90008-Z / Biochim Biophys Acta / Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity by Mollenhauer (1990)
10.1073/pnas.90.23.11307 / Proc Natl Acad Sci USA / Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity and lack of toxicity in humans by Nabel (1993)
10.1016/j.ymthe.2004.08.010 / Mol Ther / Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement by Nakase (2004)
10.1083/jcb.141.4.905 / J Cell Biol / Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains by Orlandi (1998)
10.1083/jcb.127.5.1199 / J Cell Biol / Regulated internalization of caveolae by Parton (1994)
10.1038/35074539 / Nat Cell Biol / Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER by Pelkmans (2001)
10.1074/jbc.272.11.7398 / J Biol Chem / Biochemical and functional characterization of DNA complexes capable of targeting genes to hepatocytes via the asialoglycoprotein receptor by Perales (1997)
10.1016/S1074-5521(02)00284-3 / Chem Biol / Small molecules, big impact: a history of chemical inhibitors and the cytoskeleton by Peterson (2002)
10.1016/S0021-9258(18)99963-1 / J Biol Chem / The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems by Plank (1994)
10.1074/jbc.273.13.7507 / J Biol Chem / Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells by Pollard (1998)
10.1126/science.275.5301.810 / Science (Wash DC) / Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes by Radler (1997)
10.1016/j.ymthe.2005.03.038 / Mol Ther / Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes by Rejman (2005)
10.1042/bj20031253 / Biochem J / Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis by Rejman (2004)
10.1074/jbc.M401604200 / J Biol Chem / Cellular uptake of unconjugated TAT peptide involves clathrin-dependent endocytosis and heparan sulfate receptors by Richard (2005)
10.1074/jbc.M209548200 / J Biol Chem / Cell-penetrating peptides: a reevaluation of the mechanism of cellular uptake by Richard (2003)
10.1083/jcb.105.2.679 / J Cell Biol / Acidification of the cytosol inhibits endocytosis from coated pits by Sandvig (1987)
10.1073/pnas.83.17.6425 / Proc Natl Acad Sci USA / Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells by Saraste (1986)
10.1146/annurev.biochem.66.1.511 / Annu Rev Biochem / Clathrin-coated vesicle formation and protein sorting: an integrated process by Schmid (1997)
10.1083/jcb.127.5.1217 / J Cell Biol / Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis and capillary permeability of select macromolecules by Schnitzer (1994)
10.1038/nbt0198-80 / Nat Biotechnol / DNA vector chemistry: the covalent attachment of signal peptides to plasmid DNA by Sebestyen (1998)
10.1038/sj.gt.3301015 / Gene Ther / Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides by Simoes (1999)
10.1128/MCB.19.11.7289 / Mol Cell Biol / Caveolins, liquid-ordered domains and signal transduction by Smart (1999)
10.1016/0304-4157(93)90004-8 / Biochim Biophys Acta / Liposomes as agents of DNA transfer by Smith (1993)
10.1038/ng1293-397 / Nat Genet / Adenovirus mediated expression of therapeutic plasma levels of human factor IX in mice by Smith (1993)
{'key': '10.1124/pr.58.1.8_REF101', 'first-page': '267', 'article-title': 'Trafficking of the transferrin receptor', 'volume': '4', 'author': 'Stoorvogel', 'year': '1991', 'journal-title': 'Targeted Diagn Ther'} / Targeted Diagn Ther / Trafficking of the transferrin receptor by Stoorvogel (1991)
10.1242/jcs.107.12.3461 / J Cell Sci / Rapid endocytosis of interleukin 2 receptors when clathrin-coated pit endocytosis is inhibited by Subtil (1994)
10.1016/S0962-8924(00)89101-1 / Trends Cell Biol / Macropinocytosis by Swanson (1995)
10.1016/S0169-409X(01)00211-3 / Adv Drug Deliv Rev / Quantitative studies on the nuclear transport of plasmid DNA and gene expression employing nonviral vectors by Tachibana (2001)
10.1006/bbrc.1998.9460 / Biochem Biophys Res Commun / Intracellular regulation of macromolecules using pH-sensitive liposomes and nuclear localization signal: qualitative and quantitative evaluation of intracellular trafficking by Tachibana (1998)
10.1016/S0962-8924(01)02082-7 / Trends Cell Biol / Clathrin-mediated endocytosis: membrane factors pull the trigger by Takei (2001)
10.1038/374186a0 / Nature (Lond) / Tubular membrane invaginations coated by dynamin rings are induced by GTP-γ S in nerve terminals by Takei (1995)
10.1016/0092-8674(83)90286-6 / Cell / Perturbation of vesicular traffic with the carboxylic ionophore monensin by Tartakoff (1983)
10.1016/S0006-291X(03)01135-5 / Biochem Biophys Res Commun / Uptake of analogs of penetratin, Tat(48–60) and oligoarginine in live cells by Thoren (2003)
10.1073/pnas.0435906100 / Proc Natl Acad Sci USA / Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome-DNA complexes by Torchilin (2003)
10.1016/j.ejpb.2004.02.018 / Eur J Pharm Biopharm / Chances and pitfalls of cell penetrating peptides for cellular drug delivery by Trehin (2004)
10.1074/jbc.M006701200 / J Biol Chem / Internalization of HIV-1 Tat requires cell surface heparan sulfate proteoglycans by Tyagi (2001)
10.1074/jbc.272.25.16010 / J Biol Chem / A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus by Vives (1997)
10.1038/nm996 / Nat Med / Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis by Wadia (2004)
10.1073/pnas.89.17.7934 / Proc Natl Acad Sci USA / Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle by Wagner (1992)
10.1083/jcb.123.5.1107 / J Cell Biol / Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation by Wang (1993)
10.1021/js9504752 / J Pharm Sci / Plasmid DNA is protected against ultrasonic cavitation-induced damage when complexed to cationic liposomes by Wasan (1996)
10.1016/S0169-409X(99)00066-6 / Adv Drug Deliv Rev / Endosomes, lysosomes: their implication in gene transfer by Wattiaux (2000)
10.1091/mbc.5.2.183 / Mol Biol Cell / Syndecan 4 heparan sulfate proteoglycan is a selectively enriched and widespread focal adhesion component by Woods (1994)
10.1016/0005-2736(95)80017-A / Biochim Biophys Acta / Fusion of cationic liposomes with mammalian cells occurs after endocytosis by Wrobel (1995)
10.1016/S0021-9258(18)61209-8 / J Biol Chem / Receptor-mediated in vitro gene transformation by a soluble DNA carrier system by Wu (1987)
10.1021/bi9602019 / Biochemistry / Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection by Xu (1996)
10.1073/pnas.91.10.4407 / Proc Natl Acad Sci USA / Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy by Yang (1994)
10.1074/jbc.270.32.18997 / J Biol Chem / Cellular and molecular barriers to gene transfer by a cationic lipid by Zabner (1995)
10.1038/35052055 / Nat Rev Mol Cell Biol / Rab proteins as membrane organizers by Zerial (2001)
10.1016/0005-2736(94)90066-3 / Biochim Biophys Acta / DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action by Zhou (1994)
10.1074/jbc.M111257200 / J Biol Chem / Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis by Zuhorn (2002)

Dates

Type	When
Created	19 years, 6 months ago (Feb. 28, 2006, 6:03 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 4, 2025, 3:53 p.m.)
Indexed	2 days, 20 hours ago (Aug. 26, 2025, 2:57 a.m.)
Issued	19 years, 5 months ago (March 1, 2006)
Published	19 years, 5 months ago (March 1, 2006)
Published Print	19 years, 5 months ago (March 1, 2006)

Funders 0

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BibTeX

@article{Khalil_2006, title={Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery}, volume={58}, ISSN={0031-6997}, url={http://dx.doi.org/10.1124/pr.58.1.8}, DOI={10.1124/pr.58.1.8}, number={1}, journal={Pharmacological Reviews}, publisher={Elsevier BV}, author={Khalil, Ikramy A. and Kogure, Kentaro and Akita, Hidetaka and Harashima, Hideyoshi}, year={2006}, month=mar, pages={32–45} }

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      "key": "10.1124/pr.58.1.8_REF6",
      "doi-asserted-by": "crossref",
      "first-page": "410",
      "DOI": "10.1126/science.1310359",
      "article-title": "Potocytosis: sequestration and transport of small molecules by caveolae",
      "volume": "255",
      "author": "Anderson",
      "year": "1992",
      "journal-title": "Science (Wash DC)"
    },
    {
      "issue": "Pt 2",
      "key": "10.1124/pr.58.1.8_REF7",
      "doi-asserted-by": "crossref",
      "first-page": "297",
      "DOI": "10.1042/bj2960297",
      "article-title": "Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses",
      "volume": "296",
      "author": "Arcaro",
      "year": "1993",
      "journal-title": "Biochem J"
    },
    {
      "key": "10.1124/pr.58.1.8_REF8",
      "doi-asserted-by": "crossref",
      "first-page": "1587",
      "DOI": "10.1016/S0960-9822(00)00859-9",
      "article-title": "Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton",
      "volume": "10",
      "author": "Ayscough",
      "year": "2000",
      "journal-title": "Curr Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF9",
      "doi-asserted-by": "crossref",
      "first-page": "291",
      "DOI": "10.1016/S0169-409X(99)00034-4",
      "article-title": "Biological barriers to cellular delivery of lipid-based DNA carriers",
      "volume": "38",
      "author": "Bally",
      "year": "1999",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF10",
      "first-page": "1016",
      "article-title": "Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O",
      "volume": "15",
      "author": "Barry",
      "year": "1993",
      "journal-title": "Biotechniques"
    },
    {
      "key": "10.1124/pr.58.1.8_REF11",
      "doi-asserted-by": "crossref",
      "first-page": "6982",
      "DOI": "10.1073/pnas.86.18.6982",
      "article-title": "Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA",
      "volume": "86",
      "author": "Behr",
      "year": "1989",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF12",
      "doi-asserted-by": "crossref",
      "first-page": "3567",
      "DOI": "10.1093/nar/21.15.3567",
      "article-title": "Electroporation enhances c-myc antisense oligodeoxynucleotide efficacy",
      "volume": "21",
      "author": "Bergan",
      "year": "1993",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "10.1124/pr.58.1.8_REF13",
      "doi-asserted-by": "crossref",
      "first-page": "7297",
      "DOI": "10.1073/pnas.92.16.7297",
      "article-title": "A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine",
      "volume": "92",
      "author": "Boussif",
      "year": "1995",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF14",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1146/annurev.cellbio.17.1.517",
      "article-title": "Biological basket weaving: formation and function of clathrin-coated vesicles",
      "volume": "17",
      "author": "Brodsky",
      "year": "2001",
      "journal-title": "Annu Rev Cell Dev Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF15",
      "doi-asserted-by": "crossref",
      "first-page": "559",
      "DOI": "10.1016/j.addr.2004.12.001",
      "article-title": "Tat peptide-mediated cellular delivery: back to basics",
      "volume": "57",
      "author": "Brooks",
      "year": "2005",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF16",
      "doi-asserted-by": "crossref",
      "first-page": "512",
      "DOI": "10.1002/mabi.200500018",
      "article-title": "Folate receptor-mediated gene delivery using folate-poly(ethylene glycol)-poly(l-lysine) conjugate",
      "volume": "5",
      "author": "Cho",
      "year": "2005",
      "journal-title": "Macromol Biosci"
    },
    {
      "key": "10.1124/pr.58.1.8_REF17",
      "doi-asserted-by": "crossref",
      "first-page": "1710",
      "DOI": "10.1002/jps.10437",
      "article-title": "Biophysical characterization of PEI/DNA complexes",
      "volume": "92",
      "author": "Choosakoonkriang",
      "year": "2003",
      "journal-title": "J Pharm Sci"
    },
    {
      "key": "10.1124/pr.58.1.8_REF18",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1038/nature01451",
      "article-title": "Regulated portals of entry into the cell",
      "volume": "422",
      "author": "Conner",
      "year": "2003",
      "journal-title": "Nature (Lond)"
    },
    {
      "key": "10.1124/pr.58.1.8_REF19",
      "doi-asserted-by": "crossref",
      "first-page": "4033",
      "DOI": "10.1073/pnas.87.11.4033",
      "article-title": "Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels",
      "volume": "87",
      "author": "Cotten",
      "year": "1990",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF20",
      "doi-asserted-by": "crossref",
      "first-page": "127",
      "DOI": "10.1016/0009-3084(86)90067-8",
      "article-title": "Lipid polymorphism and the roles of lipids in membranes",
      "volume": "40",
      "author": "Cullis",
      "year": "1986",
      "journal-title": "Chem Phys Lipids"
    },
    {
      "key": "10.1124/pr.58.1.8_REF21",
      "doi-asserted-by": "crossref",
      "first-page": "915",
      "DOI": "10.1083/jcb.127.4.915",
      "article-title": "Induction of mutant dynamin specifically blocks endocytic coated vesicle formation",
      "volume": "127",
      "author": "Damke",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF22",
      "doi-asserted-by": "crossref",
      "first-page": "2495",
      "DOI": "10.1016/0006-2952(74)90174-9",
      "article-title": "Commentary: lysosomotropic agents",
      "volume": "23",
      "author": "de Duve",
      "year": "1974",
      "journal-title": "Biochem Pharmacol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF23",
      "doi-asserted-by": "crossref",
      "first-page": "18188",
      "DOI": "10.1074/jbc.271.30.18188",
      "article-title": "Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent",
      "volume": "271",
      "author": "Derossi",
      "year": "1996",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF24",
      "doi-asserted-by": "crossref",
      "first-page": "35",
      "DOI": "10.1016/S0169-409X(00)00082-X",
      "article-title": "Novel non-endocytic delivery of antisense oligonucleotides",
      "volume": "44",
      "author": "Dokka",
      "year": "2000",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF25",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1242/jeb.200.1.1",
      "article-title": "Bafilomycins and concanamycins as inhibitors of V-ATPases and P-ATPases",
      "volume": "200",
      "author": "Drose",
      "year": "1997",
      "journal-title": "J Exp Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF26",
      "doi-asserted-by": "crossref",
      "first-page": "1279",
      "DOI": "10.1083/jcb.107.4.1279",
      "article-title": "The effects of variations in the number and sequence of targeting signals on nuclear uptake",
      "volume": "107",
      "author": "Dworetzky",
      "year": "1988",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF27",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1016/S0005-2736(96)00242-8",
      "article-title": "Biophysical characterization of cationic lipid: DNA complexes",
      "volume": "1325",
      "author": "Eastman",
      "year": "1997",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF28",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1016/0005-2736(95)80016-9",
      "article-title": "The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer",
      "volume": "1235",
      "author": "Farhood",
      "year": "1995",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF29",
      "doi-asserted-by": "crossref",
      "first-page": "7413",
      "DOI": "10.1073/pnas.84.21.7413",
      "article-title": "Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure",
      "volume": "84",
      "author": "Felgner",
      "year": "1987",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF30",
      "doi-asserted-by": "crossref",
      "first-page": "126",
      "DOI": "10.1111/j.1749-6632.1995.tb44738.x",
      "article-title": "Improved cationic lipid formulations for in vivo gene therapy",
      "volume": "772",
      "author": "Felgner",
      "year": "1995",
      "journal-title": "Ann NY Acad Sci"
    },
    {
      "key": "10.1124/pr.58.1.8_REF31",
      "doi-asserted-by": "crossref",
      "first-page": "284",
      "DOI": "10.1016/S1525-0016(03)00122-9",
      "article-title": "Caveolae-mediated internalization of extracellular HIV-1 tat fusion proteins visualized in real time",
      "volume": "8",
      "author": "Ferrari",
      "year": "2003",
      "journal-title": "Mol Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF32",
      "doi-asserted-by": "crossref",
      "first-page": "34141",
      "DOI": "10.1074/jbc.M303045200",
      "article-title": "Cell membrane lipid rafts mediate caveolar endocytosis of HIV-1 Tat fusion proteins",
      "volume": "278",
      "author": "Fittipaldi",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF33",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1016/0005-2736(95)00219-7",
      "article-title": "Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes",
      "volume": "1278",
      "author": "Friend",
      "year": "1996",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF34",
      "doi-asserted-by": "crossref",
      "first-page": "161",
      "DOI": "10.1034/j.1600-0854.2000.010208.x",
      "article-title": "Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis in mammalian cells",
      "volume": "1",
      "author": "Fujimoto",
      "year": "2000",
      "journal-title": "Traffic"
    },
    {
      "key": "10.1124/pr.58.1.8_REF35",
      "doi-asserted-by": "crossref",
      "first-page": "1005",
      "DOI": "10.1021/bc015508l",
      "article-title": "Stearylated arginine-rich peptides: a new class of transfection systems",
      "volume": "12",
      "author": "Futaki",
      "year": "2001",
      "journal-title": "Bioconjug Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF36",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1146/annurev.cb.01.110185.000245",
      "article-title": "Receptor-mediated endocytosis: concepts emerging from the LDL receptor system",
      "volume": "1",
      "author": "Goldstein",
      "year": "1985",
      "journal-title": "Annu Rev Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF37",
      "doi-asserted-by": "crossref",
      "first-page": "373",
      "DOI": "10.1016/j.ymthe.2004.05.023",
      "article-title": "Macropinocytosis of polyplexes and recycling of plasmid via the clathrindependent pathway impair the transfection efficiency of human hepatocarcinoma cells",
      "volume": "10",
      "author": "Goncalves",
      "year": "2004",
      "journal-title": "Mol Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF38",
      "doi-asserted-by": "crossref",
      "first-page": "637",
      "DOI": "10.1016/j.addr.2004.10.007",
      "article-title": "Intracellular delivery of large molecules and small particles by cell-penetrating proteins and peptides",
      "volume": "57",
      "author": "Gupta",
      "year": "2005",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF39",
      "doi-asserted-by": "crossref",
      "first-page": "158",
      "DOI": "10.1016/S1471-4906(01)02161-5",
      "article-title": "Caveolae and caveolin in immune cells: distribution and functions",
      "volume": "23",
      "author": "Harris",
      "year": "2002",
      "journal-title": "Trends Immunol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF40",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1083/jcb.108.2.389",
      "article-title": "Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation",
      "volume": "108",
      "author": "Heuser",
      "year": "1989",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF41",
      "doi-asserted-by": "crossref",
      "first-page": "689",
      "DOI": "10.1083/jcb.124.5.689",
      "article-title": "The coated pit and macropinocytic pathways serve distinct endosome populations",
      "volume": "124",
      "author": "Hewlett",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF42",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/0925-4439(91)90016-3",
      "article-title": "Liposomes for the transformation of eukaryotic cells",
      "volume": "1097",
      "author": "Hug",
      "year": "1991",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF43",
      "doi-asserted-by": "crossref",
      "first-page": "633",
      "DOI": "10.1139/o96-068",
      "article-title": "Spermidine-condensed DNA and cone-shaped lipids improve delivery and expression of exogenous DNA transfer by liposomes",
      "volume": "74",
      "author": "Ibanez",
      "year": "1996",
      "journal-title": "Biochem Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF44",
      "doi-asserted-by": "crossref",
      "first-page": "651",
      "DOI": "10.1152/physrev.1996.76.3.651",
      "article-title": "Regulation of protein transport to the nucleus: central role of phosphorylation",
      "volume": "76",
      "author": "Jans",
      "year": "1996",
      "journal-title": "Physiol Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF45",
      "doi-asserted-by": "crossref",
      "first-page": "1251",
      "DOI": "10.1091/mbc.4.12.1251",
      "article-title": "Endosome acidification and receptor trafficking: bafilomycin A1 slows receptor externalization by a mechanism involving the receptor\u2019s internalization motif",
      "volume": "4",
      "author": "Johnson",
      "year": "1993",
      "journal-title": "Mol Biol Cell"
    },
    {
      "key": "10.1124/pr.58.1.8_REF46",
      "doi-asserted-by": "crossref",
      "first-page": "5618",
      "DOI": "10.1021/bi035802w",
      "article-title": "Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system",
      "volume": "43",
      "author": "Kakudo",
      "year": "2004",
      "journal-title": "Biochemistry"
    },
    {
      "key": "10.1124/pr.58.1.8_REF47",
      "doi-asserted-by": "crossref",
      "first-page": "990",
      "DOI": "10.1016/S1359-6446(03)02889-7",
      "article-title": "Pharmacokinetic and pharmacodynamic considerations in gene therapy",
      "volume": "8",
      "author": "Kamiya",
      "year": "2003",
      "journal-title": "Drug Discov Today"
    },
    {
      "key": "10.1124/pr.58.1.8_REF48",
      "doi-asserted-by": "crossref",
      "first-page": "591",
      "DOI": "10.1016/S0006-291X(02)02485-3",
      "article-title": "Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes",
      "volume": "298",
      "author": "Kamiya",
      "year": "2002",
      "journal-title": "Biochem Biophys Res Commun"
    },
    {
      "key": "10.1124/pr.58.1.8_REF49",
      "doi-asserted-by": "crossref",
      "first-page": "247",
      "DOI": "10.1016/j.jconrel.2004.10.018",
      "article-title": "Cationic TAT peptide transduction domain enters cells by macropinocytosis",
      "volume": "102",
      "author": "Kaplan",
      "year": "2005",
      "journal-title": "J Control Release"
    },
    {
      "key": "10.1124/pr.58.1.8_REF50",
      "first-page": "405",
      "article-title": "Effect of cationic charge on receptor-mediated transfection using mannosylated cationic liposome/plasmid DNA complexes following the intravenous administration in mice",
      "volume": "59",
      "author": "Kawakami",
      "year": "2004",
      "journal-title": "Pharmazie"
    },
    {
      "key": "10.1124/pr.58.1.8_REF51",
      "doi-asserted-by": "crossref",
      "first-page": "636",
      "DOI": "10.1038/sj.gt.3302128",
      "article-title": "Mechanism of improved gene transfer by the N-terminal stearylation of octaarginine: enhanced cellular association by hydrophobic core formation",
      "volume": "11",
      "author": "Khalil",
      "year": "2004",
      "journal-title": "Gene Ther"
    },
    {
      "doi-asserted-by": "crossref",
      "unstructured": "Khalil IA, Kogure K, Futaki S, and Harashima H (2006) High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression. J Biol Chem, in press.",
      "key": "10.1124/pr.58.1.8_REF52",
      "DOI": "10.1074/jbc.M503202200"
    },
    {
      "key": "10.1124/pr.58.1.8_REF53",
      "doi-asserted-by": "crossref",
      "first-page": "135",
      "DOI": "10.1002/jgm.173",
      "article-title": "Polyethylenimine-mediated gene delivery: a mechanistic study",
      "volume": "3",
      "author": "Kichler",
      "year": "2001",
      "journal-title": "J Gene Med"
    },
    {
      "key": "10.1124/pr.58.1.8_REF54",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1016/S0169-409X(97)00132-4",
      "article-title": "Identification of proteins mediating clearance of liposomes using a liver perfusion system",
      "volume": "32",
      "author": "Kiwada",
      "year": "1998",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF55",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1016/S0006-2952(98)00098-7",
      "article-title": "Effects of polyethyleneimine on endocytosis and lysosome stability",
      "volume": "56",
      "author": "Klemm",
      "year": "1998",
      "journal-title": "Biochem Pharmacol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF56",
      "doi-asserted-by": "crossref",
      "first-page": "43",
      "DOI": "10.1038/353043a0",
      "article-title": "Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents",
      "volume": "353",
      "author": "Kleuss",
      "year": "1991",
      "journal-title": "Nature (Lond)"
    },
    {
      "key": "10.1124/pr.58.1.8_REF57",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1016/j.jconrel.2004.04.024",
      "article-title": "Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method",
      "volume": "98",
      "author": "Kogure",
      "year": "2004",
      "journal-title": "J Control Release"
    },
    {
      "key": "10.1124/pr.58.1.8_REF58",
      "doi-asserted-by": "crossref",
      "first-page": "769",
      "DOI": "10.1002/jgm.558",
      "article-title": "A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin",
      "volume": "6",
      "author": "Kopatz",
      "year": "2004",
      "journal-title": "J Gene Med"
    },
    {
      "key": "10.1124/pr.58.1.8_REF59",
      "first-page": "1010",
      "article-title": "An electron microscopy study into the mechanism of gene transfer with lipopolyamines",
      "volume": "3",
      "author": "Labat-Moleur",
      "year": "1996",
      "journal-title": "Gene Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF60",
      "doi-asserted-by": "crossref",
      "first-page": "573",
      "DOI": "10.1016/0955-0674(95)80015-8",
      "article-title": "The emergence of clathrin-independent pinocytic pathways",
      "volume": "7",
      "author": "Lamaze",
      "year": "1995",
      "journal-title": "Curr Opin Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF61",
      "doi-asserted-by": "crossref",
      "first-page": "273",
      "DOI": "10.1016/0092-8674(83)90356-2",
      "article-title": "Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblasts",
      "volume": "33",
      "author": "Larkin",
      "year": "1983",
      "journal-title": "Cell"
    },
    {
      "key": "10.1124/pr.58.1.8_REF62",
      "doi-asserted-by": "crossref",
      "first-page": "626",
      "DOI": "10.1016/0958-1669(94)90085-X",
      "article-title": "Non-viral gene therapy",
      "volume": "5",
      "author": "Ledley",
      "year": "1994",
      "journal-title": "Curr Opin Biotechnol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF63",
      "doi-asserted-by": "crossref",
      "first-page": "8481",
      "DOI": "10.1074/jbc.271.14.8481",
      "article-title": "Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer",
      "volume": "271",
      "author": "Lee",
      "year": "1996",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF64",
      "doi-asserted-by": "crossref",
      "first-page": "2702",
      "DOI": "10.1073/pnas.88.7.2702",
      "article-title": "Intracellular distribution of microinjected antisense oligonucleotides",
      "volume": "88",
      "author": "Leonetti",
      "year": "1991",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF65",
      "doi-asserted-by": "crossref",
      "first-page": "31",
      "DOI": "10.1038/sj.gt.3301110",
      "article-title": "Nonviral gene therapy: promises and challenges",
      "volume": "7",
      "author": "Li",
      "year": "2000",
      "journal-title": "Gene Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF66",
      "doi-asserted-by": "crossref",
      "first-page": "967",
      "DOI": "10.1016/j.addr.2003.10.041",
      "article-title": "GALA: a designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery",
      "volume": "56",
      "author": "Li",
      "year": "2004",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF67",
      "doi-asserted-by": "crossref",
      "first-page": "167",
      "DOI": "10.1038/nbt0297-167",
      "article-title": "Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery",
      "volume": "15",
      "author": "Liu",
      "year": "1997",
      "journal-title": "Nat Biotechnol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF68",
      "doi-asserted-by": "crossref",
      "first-page": "143",
      "DOI": "10.1016/S1525-0016(03)00135-7",
      "article-title": "Cell surface adherence and endocytosis of protein transduction domains",
      "volume": "8",
      "author": "Lundberg",
      "year": "2003",
      "journal-title": "Mol Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF69",
      "doi-asserted-by": "crossref",
      "first-page": "1117",
      "DOI": "10.1074/jbc.272.2.1117",
      "article-title": "Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells",
      "volume": "272",
      "author": "Matsui",
      "year": "1997",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF70",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1016/S0169-409X(01)00138-7",
      "article-title": "The role of caveolae and caveolin in vesicle-dependent and vesicle-independent trafficking",
      "volume": "49",
      "author": "Matveev",
      "year": "2001",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF71",
      "doi-asserted-by": "crossref",
      "first-page": "121",
      "DOI": "10.1038/nrm1315",
      "article-title": "Endocytic recycling",
      "volume": "5",
      "author": "Maxfield",
      "year": "2004",
      "journal-title": "Nat Rev Mol Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF72",
      "doi-asserted-by": "crossref",
      "first-page": "451",
      "DOI": "10.1002/jgm.409",
      "article-title": "Adenovirus endocytosis",
      "volume": "5",
      "author": "Meier",
      "year": "2003",
      "journal-title": "J Gene Med"
    },
    {
      "key": "10.1124/pr.58.1.8_REF73",
      "doi-asserted-by": "crossref",
      "first-page": "310",
      "DOI": "10.1016/0955-0674(95)80084-0",
      "article-title": "Mechanisms of nuclear protein import",
      "volume": "7",
      "author": "Melchior",
      "year": "1995",
      "journal-title": "Curr Opin Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF74",
      "doi-asserted-by": "crossref",
      "first-page": "663",
      "DOI": "10.1146/annurev.bi.55.070186.003311",
      "article-title": "Acidification of the endocytic and exocytic pathways",
      "volume": "55",
      "author": "Mellman",
      "year": "1986",
      "journal-title": "Annu Rev Biochem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF75",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1016/0005-2736(95)00163-W",
      "article-title": "Membrane permeabilization by \u03b1-helical peptides: a flow cytometry study",
      "volume": "1239",
      "author": "Midoux",
      "year": "1995",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF76",
      "doi-asserted-by": "crossref",
      "first-page": "12349",
      "DOI": "10.1073/pnas.93.22.12349",
      "article-title": "Evidence for the role of proteoglycans in cation-mediated gene transfer",
      "volume": "93",
      "author": "Mislick",
      "year": "1996",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF77",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1016/0304-4157(90)90008-Z",
      "article-title": "Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity",
      "volume": "1031",
      "author": "Mollenhauer",
      "year": "1990",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF78",
      "doi-asserted-by": "crossref",
      "first-page": "11307",
      "DOI": "10.1073/pnas.90.23.11307",
      "article-title": "Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity and lack of toxicity in humans",
      "volume": "90",
      "author": "Nabel",
      "year": "1993",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF79",
      "doi-asserted-by": "crossref",
      "first-page": "1011",
      "DOI": "10.1016/j.ymthe.2004.08.010",
      "article-title": "Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement",
      "volume": "10",
      "author": "Nakase",
      "year": "2004",
      "journal-title": "Mol Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF80",
      "doi-asserted-by": "crossref",
      "first-page": "905",
      "DOI": "10.1083/jcb.141.4.905",
      "article-title": "Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains",
      "volume": "141",
      "author": "Orlandi",
      "year": "1998",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF81",
      "doi-asserted-by": "crossref",
      "first-page": "1199",
      "DOI": "10.1083/jcb.127.5.1199",
      "article-title": "Regulated internalization of caveolae",
      "volume": "127",
      "author": "Parton",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF82",
      "doi-asserted-by": "crossref",
      "first-page": "473",
      "DOI": "10.1038/35074539",
      "article-title": "Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER",
      "volume": "3",
      "author": "Pelkmans",
      "year": "2001",
      "journal-title": "Nat Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF83",
      "doi-asserted-by": "crossref",
      "first-page": "7398",
      "DOI": "10.1074/jbc.272.11.7398",
      "article-title": "Biochemical and functional characterization of DNA complexes capable of targeting genes to hepatocytes via the asialoglycoprotein receptor",
      "volume": "272",
      "author": "Perales",
      "year": "1997",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF84",
      "doi-asserted-by": "crossref",
      "first-page": "1275",
      "DOI": "10.1016/S1074-5521(02)00284-3",
      "article-title": "Small molecules, big impact: a history of chemical inhibitors and the cytoskeleton",
      "volume": "9",
      "author": "Peterson",
      "year": "2002",
      "journal-title": "Chem Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF85",
      "doi-asserted-by": "crossref",
      "first-page": "12918",
      "DOI": "10.1016/S0021-9258(18)99963-1",
      "article-title": "The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems",
      "volume": "269",
      "author": "Plank",
      "year": "1994",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF86",
      "doi-asserted-by": "crossref",
      "first-page": "7507",
      "DOI": "10.1074/jbc.273.13.7507",
      "article-title": "Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells",
      "volume": "273",
      "author": "Pollard",
      "year": "1998",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF87",
      "doi-asserted-by": "crossref",
      "first-page": "810",
      "DOI": "10.1126/science.275.5301.810",
      "article-title": "Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes",
      "volume": "275",
      "author": "Radler",
      "year": "1997",
      "journal-title": "Science (Wash DC)"
    },
    {
      "key": "10.1124/pr.58.1.8_REF88",
      "doi-asserted-by": "crossref",
      "first-page": "468",
      "DOI": "10.1016/j.ymthe.2005.03.038",
      "article-title": "Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes",
      "volume": "12",
      "author": "Rejman",
      "year": "2005",
      "journal-title": "Mol Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF89",
      "doi-asserted-by": "crossref",
      "first-page": "159",
      "DOI": "10.1042/bj20031253",
      "article-title": "Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis",
      "volume": "377",
      "author": "Rejman",
      "year": "2004",
      "journal-title": "Biochem J"
    },
    {
      "key": "10.1124/pr.58.1.8_REF90",
      "doi-asserted-by": "crossref",
      "first-page": "15300",
      "DOI": "10.1074/jbc.M401604200",
      "article-title": "Cellular uptake of unconjugated TAT peptide involves clathrin-dependent endocytosis and heparan sulfate receptors",
      "volume": "280",
      "author": "Richard",
      "year": "2005",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF91",
      "doi-asserted-by": "crossref",
      "first-page": "585",
      "DOI": "10.1074/jbc.M209548200",
      "article-title": "Cell-penetrating peptides: a reevaluation of the mechanism of cellular uptake",
      "volume": "278",
      "author": "Richard",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF92",
      "doi-asserted-by": "crossref",
      "first-page": "679",
      "DOI": "10.1083/jcb.105.2.679",
      "article-title": "Acidification of the cytosol inhibits endocytosis from coated pits",
      "volume": "105",
      "author": "Sandvig",
      "year": "1987",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF93",
      "doi-asserted-by": "crossref",
      "first-page": "6425",
      "DOI": "10.1073/pnas.83.17.6425",
      "article-title": "Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells",
      "volume": "83",
      "author": "Saraste",
      "year": "1986",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF94",
      "doi-asserted-by": "crossref",
      "first-page": "511",
      "DOI": "10.1146/annurev.biochem.66.1.511",
      "article-title": "Clathrin-coated vesicle formation and protein sorting: an integrated process",
      "volume": "66",
      "author": "Schmid",
      "year": "1997",
      "journal-title": "Annu Rev Biochem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF95",
      "doi-asserted-by": "crossref",
      "first-page": "1217",
      "DOI": "10.1083/jcb.127.5.1217",
      "article-title": "Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis and capillary permeability of select macromolecules",
      "volume": "127",
      "author": "Schnitzer",
      "year": "1994",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF96",
      "doi-asserted-by": "crossref",
      "first-page": "80",
      "DOI": "10.1038/nbt0198-80",
      "article-title": "DNA vector chemistry: the covalent attachment of signal peptides to plasmid DNA",
      "volume": "16",
      "author": "Sebestyen",
      "year": "1998",
      "journal-title": "Nat Biotechnol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF97",
      "doi-asserted-by": "crossref",
      "first-page": "1798",
      "DOI": "10.1038/sj.gt.3301015",
      "article-title": "Mechanisms of gene transfer mediated by lipoplexes associated with targeting ligands or pH-sensitive peptides",
      "volume": "6",
      "author": "Simoes",
      "year": "1999",
      "journal-title": "Gene Ther"
    },
    {
      "key": "10.1124/pr.58.1.8_REF98",
      "doi-asserted-by": "crossref",
      "first-page": "7289",
      "DOI": "10.1128/MCB.19.11.7289",
      "article-title": "Caveolins, liquid-ordered domains and signal transduction",
      "volume": "19",
      "author": "Smart",
      "year": "1999",
      "journal-title": "Mol Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF99",
      "doi-asserted-by": "crossref",
      "first-page": "327",
      "DOI": "10.1016/0304-4157(93)90004-8",
      "article-title": "Liposomes as agents of DNA transfer",
      "volume": "1154",
      "author": "Smith",
      "year": "1993",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "10.1124/pr.58.1.8_REF100",
      "doi-asserted-by": "crossref",
      "first-page": "397",
      "DOI": "10.1038/ng1293-397",
      "article-title": "Adenovirus mediated expression of therapeutic plasma levels of human factor IX in mice",
      "volume": "5",
      "author": "Smith",
      "year": "1993",
      "journal-title": "Nat Genet"
    },
    {
      "key": "10.1124/pr.58.1.8_REF101",
      "first-page": "267",
      "article-title": "Trafficking of the transferrin receptor",
      "volume": "4",
      "author": "Stoorvogel",
      "year": "1991",
      "journal-title": "Targeted Diagn Ther"
    },
    {
      "issue": "Pt 12",
      "key": "10.1124/pr.58.1.8_REF102",
      "doi-asserted-by": "crossref",
      "first-page": "3461",
      "DOI": "10.1242/jcs.107.12.3461",
      "article-title": "Rapid endocytosis of interleukin 2 receptors when clathrin-coated pit endocytosis is inhibited",
      "volume": "107",
      "author": "Subtil",
      "year": "1994",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "10.1124/pr.58.1.8_REF103",
      "doi-asserted-by": "crossref",
      "first-page": "424",
      "DOI": "10.1016/S0962-8924(00)89101-1",
      "article-title": "Macropinocytosis",
      "volume": "5",
      "author": "Swanson",
      "year": "1995",
      "journal-title": "Trends Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF104",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1016/S0169-409X(01)00211-3",
      "article-title": "Quantitative studies on the nuclear transport of plasmid DNA and gene expression employing nonviral vectors",
      "volume": "52",
      "author": "Tachibana",
      "year": "2001",
      "journal-title": "Adv Drug Deliv Rev"
    },
    {
      "key": "10.1124/pr.58.1.8_REF105",
      "doi-asserted-by": "crossref",
      "first-page": "538",
      "DOI": "10.1006/bbrc.1998.9460",
      "article-title": "Intracellular regulation of macromolecules using pH-sensitive liposomes and nuclear localization signal: qualitative and quantitative evaluation of intracellular trafficking",
      "volume": "251",
      "author": "Tachibana",
      "year": "1998",
      "journal-title": "Biochem Biophys Res Commun"
    },
    {
      "key": "10.1124/pr.58.1.8_REF106",
      "doi-asserted-by": "crossref",
      "first-page": "385",
      "DOI": "10.1016/S0962-8924(01)02082-7",
      "article-title": "Clathrin-mediated endocytosis: membrane factors pull the trigger",
      "volume": "11",
      "author": "Takei",
      "year": "2001",
      "journal-title": "Trends Cell Biol"
    },
    {
      "key": "10.1124/pr.58.1.8_REF107",
      "doi-asserted-by": "crossref",
      "first-page": "186",
      "DOI": "10.1038/374186a0",
      "article-title": "Tubular membrane invaginations coated by dynamin rings are induced by GTP-\u03b3 S in nerve terminals",
      "volume": "374",
      "author": "Takei",
      "year": "1995",
      "journal-title": "Nature (Lond)"
    },
    {
      "key": "10.1124/pr.58.1.8_REF108",
      "doi-asserted-by": "crossref",
      "first-page": "1026",
      "DOI": "10.1016/0092-8674(83)90286-6",
      "article-title": "Perturbation of vesicular traffic with the carboxylic ionophore monensin",
      "volume": "32",
      "author": "Tartakoff",
      "year": "1983",
      "journal-title": "Cell"
    },
    {
      "key": "10.1124/pr.58.1.8_REF109",
      "doi-asserted-by": "crossref",
      "first-page": "100",
      "DOI": "10.1016/S0006-291X(03)01135-5",
      "article-title": "Uptake of analogs of penetratin, Tat(48\u201360) and oligoarginine in live cells",
      "volume": "307",
      "author": "Thoren",
      "year": "2003",
      "journal-title": "Biochem Biophys Res Commun"
    },
    {
      "key": "10.1124/pr.58.1.8_REF110",
      "doi-asserted-by": "crossref",
      "first-page": "1972",
      "DOI": "10.1073/pnas.0435906100",
      "article-title": "Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome-DNA complexes",
      "volume": "100",
      "author": "Torchilin",
      "year": "2003",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "10.1124/pr.58.1.8_REF111",
      "doi-asserted-by": "crossref",
      "first-page": "209",
      "DOI": "10.1016/j.ejpb.2004.02.018",
      "article-title": "Chances and pitfalls of cell penetrating peptides for cellular drug delivery",
      "volume": "58",
      "author": "Trehin",
      "year": "2004",
      "journal-title": "Eur J Pharm Biopharm"
    },
    {
      "key": "10.1124/pr.58.1.8_REF112",
      "doi-asserted-by": "crossref",
      "first-page": "3254",
      "DOI": "10.1074/jbc.M006701200",
      "article-title": "Internalization of HIV-1 Tat requires cell surface heparan sulfate proteoglycans",
      "volume": "276",
      "author": "Tyagi",
      "year": "2001",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF113",
      "doi-asserted-by": "crossref",
      "first-page": "16010",
      "DOI": "10.1074/jbc.272.25.16010",
      "article-title": "A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus",
      "volume": "272",
      "author": "Vives",
      "year": "1997",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "10.1124/pr.58.1.8_REF114",
      "doi-asserted-by": "crossref",
      "first-page": "310",
      "DOI": "10.1038/nm996",
      "article-title": "Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis",
      "volume": "10",
      "author": "Wadia",
      "year": "2004",
      "journal-title": "Nat Med"
    },
    {
      "key": "10.1124/pr.58.1.8_REF115",
      "doi-asserted-by": "crossref",
      "first-page": "7934",
      "DOI": "10.1073/pnas.89.17.7934",
      "article-title": "Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle",
      "volume": "89",
      "author": "Wagner",
      "year": "1992",
      "journal-title": "Proc Natl Acad Sci USA"
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      "author": "Wang",
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      "author": "Wasan",
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      "author": "Wu",
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