DOI: 10.1113/jphysiol.2004.062471. Cl<sup>−</sup> uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1

Cl− uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1

10.1113/jphysiol.2004.062471

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

GABA is the principal inhibitory neurotransmitter in the mature brain, but during early postnatal development the elevated [Cl−]i in immature neocortical neurones causes GABAA receptor activation to be depolarizing. The molecular mechanisms underlying this intracellular Cl− accumulation remain controversial. Therefore, the GABA reversal potential (EGABA) or [Cl−]i in early postnatal rat neocortical neurones was measured by the gramicidin‐perforated patch‐clamp method, and the relative expression levels of the cation−Cl− cotransporter mRNAs (in the same cells) were examined by semiquantitative single‐cell multiplex RT‐PCR to look for statistical correlations with [Cl−]i. The mRNA expression levels were positively (the Cl− accumulating Na+,K+−2Cl− cotransporter NKCC1) or negatively (the Cl− extruding K+−Cl− cotransporter KCC2) correlated with [Cl−]i. NKCC1 mRNA expression was high in early postnatal days, but decreased during postnatal development, whereas KCC2 mRNA expression displayed the opposite pattern. [Cl−]i and NKCC1 mRNA expression were each higher in cortical plate (CP) neurones than in the presumably older layer V/VI pyramidal neurones in a given slice. The pharmacological effects of bumetanide on EGABA were consistent with the different expression levels of NKCC1 mRNA. These data suggest that NKCC1 may play a pivotal role in the generation of GABA‐mediated depolarization in immature CP cells, while KCC2 promotes the later maturation of GABAergic inhibition in the rat neocortex.

Bibliography

Yamada, J., Okabe, A., Toyoda, H., Kilb, W., Luhmann, H. J., & Fukuda, A. (2004). Clâ uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1. The Journal of Physiology, 557(3), 829â841. Portico.

Authors 6

Junko Yamada (first)
Akihito Okabe (additional)
Hiroki Toyoda (additional)
Werner Kilb (additional)
Heiko J. Luhmann (additional)
Atsuo Fukuda (additional)

References 48 Referenced 469

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Dates

Type	When
Created	21 years, 4 months ago (April 19, 2004, 8:45 p.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 3:33 p.m.)
Indexed	5 days, 4 hours ago (Aug. 23, 2025, 9:20 p.m.)
Issued	21 years, 2 months ago (June 1, 2004)
Published	21 years, 2 months ago (June 1, 2004)
Published Online	21 years, 2 months ago (June 14, 2004)
Published Print	21 years, 2 months ago (June 1, 2004)

Funders 0

None

BibTeX

@article{Yamada_2004, title={Cl− uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1}, volume={557}, ISSN={1469-7793}, url={http://dx.doi.org/10.1113/jphysiol.2004.062471}, DOI={10.1113/jphysiol.2004.062471}, number={3}, journal={The Journal of Physiology}, publisher={Wiley}, author={Yamada, Junko and Okabe, Akihito and Toyoda, Hiroki and Kilb, Werner and Luhmann, Heiko J. and Fukuda, Atsuo}, year={2004}, month=jun, pages={829–841} }

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    "0022-3751",
    "1469-7793"
  ],
  "subject": [],
  "container-title-short": "The Journal of Physiology",
  "published": {
    "date-parts": [
      [
        2004,
        6
      ]
    ]
  }
}