DOI: 10.1113/jphysiol.2002.030924. Functional analysis of neurotransmission at β2‐laminin deficient terminals

Functional analysis of neurotransmission at β2‐laminin deficient terminals

10.1113/jphysiol.2002.030924

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

β2‐Laminin is important for the formation of neuromuscular junctions in vertebrates. Previously, we have inactivated the gene that encodes for β2‐laminin in mice and observed predominantly prejunctional structural defects. In this study, we have used both intra‐ and extracellular recording methods to investigate evoked neurotransmission in β2‐laminin‐deficient mice, from postnatal day 8 (P8) through to day 18 (P18). Our results confirmed that there was a decrease in the frequency of spontaneous release, but no change in the postjunctional response to such release. Analysis of evoked neurotransmission showed an increase in the frequency of stimuli that failed to elicit an evoked postjunctional response in the mutants compared to litter mate controls, resulting in a 50 % reduction in mean quantal content at mutant terminals. Compared to littermate controls, β2‐laminin‐deficient terminals showed greater synaptic depression when subjected to high frequency stimulation. Furthermore, the paired pulse ratio of the first two stimuli was significantly lower in β2‐laminin mutant terminals. Statistical analysis of the binomial parameters of release showed that the decrease in quantal content was due to a decrease in the number of release sites without any significant change in the average probability of release. This suggestion was supported by the observation of fewer synaptic vesicle protein 2 (SV2)‐positive varicosities in β2‐laminin‐deficient terminals and by ultrastructural observations showing smaller terminal profiles and increased Schwann cell invasion in β2‐laminin mutants; the differences between β2‐laminin mutants and wild‐type mice were the same at both P8 and P18. From these results we conclude that β2‐laminin plays a role in the early structural development of the neuromuscular junction. We also suggest that transmitter release activity may act as a deterrent to Schwann cell invasion in the absence of β2‐laminin.

Authors 5

David Knight (first)
Lynn K. Tolley (additional)
David K. Kim (additional)
Nick A. Lavidis (additional)
Peter G. Noakes (additional)

References 46 Referenced 58

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Dates

Type	When
Created	22 years, 7 months ago (Feb. 3, 2003, 3:47 p.m.)
Deposited	2 years ago (Sept. 4, 2023, 5:26 p.m.)
Indexed	1 year, 2 months ago (June 8, 2024, 5:18 p.m.)
Issued	22 years, 7 months ago (Feb. 1, 2003)
Published	22 years, 7 months ago (Feb. 1, 2003)
Published Online	22 years, 7 months ago (Feb. 1, 2003)
Published Print	22 years, 7 months ago (Feb. 1, 2003)

Funders 0

None

BibTeX

@article{Knight_2003, title={Functional analysis of neurotransmission at β2‐laminin deficient terminals}, volume={546}, ISSN={1469-7793}, url={http://dx.doi.org/10.1113/jphysiol.2002.030924}, DOI={10.1113/jphysiol.2002.030924}, number={3}, journal={The Journal of Physiology}, publisher={Wiley}, author={Knight, David and Tolley, Lynn K. and Kim, David K. and Lavidis, Nick A. and Noakes, Peter G.}, year={2003}, month=feb, pages={789–800} }

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