DOI: 10.1113/jphysiol.2002.022418. Excitation‐contraction coupling and sarcoplasmic reticulum function in lechanically skinned fibres from fast skeletal muscles of aged mice

Excitation‐contraction coupling and sarcoplasmic reticulum function in lechanically skinned fibres from fast skeletal muscles of aged mice

10.1113/jphysiol.2002.022418

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

Ageing is generally associated with a decline in skeletal muscle mass and strength, and a slowing of muscle contraction, factors that impact upon the quality of life for the elderly. Alterations in Ca2+ handling are thought to contribute to these age‐related changes in muscle contractility, yet the effects of ageing on sarcoplasmic reticulum (SR) Ca2+ handling and the Ca2+ transport system remain unresolved. We used mechanically skinned single fibres from the fast twitch extensor digitorum longus (EDL) muscles from young (4‐month‐old) and old (27‐ to 28‐month‐old) mice to test the hypothesis that the age‐related changes in skeletal muscle contractility, especially the slower rate of contraction, are due to changes intrinsic to the muscle fibres. There were no age‐related differences in the peak height of depolarization‐induced contractile response (DICR) or the number of DICRs elicited before rundown (DICR < 50 % of initial). The time taken to reach peak DICR (TPDICR) was ≈12 % slower in single muscle fibres from old compared with young mice (P < 0.05). The rate of relaxation following DICR was not different in young and old mice. Examination of SR function demonstrated that SR Ca2+ reloading in Ca2+‐depleted skinned fibres was not different in young and old mice, nor was there any age‐related difference in Ca2+ leak from the SR. However, low [caffeine] contracture in fibres from old mice was only half of that observed in fibres from young mice (P < 0.05), indicating a lower sensitivity of the SR Ca2+ release channel (CRC) to caffeine. We found no difference in maximum Ca2+‐activated force (Po) or specific force (sPo; Po corrected for cross‐sectional area) in EDL muscle fibres from young and old mice. Impaired excitation‐contraction (E‐C) coupling and a decrease in SR CRC function are mechanisms which are likely to contribute to the overall slowing of muscle contraction with age.

Bibliography

Plant, D. R., & Lynch, G. S. (2002). Excitationâcontraction coupling and sarcoplasmic reticulum function in lechanically skinned fibres from fast skeletal muscles of aged mice. The Journal of Physiology, 543(1), 169â176. Portico.

Authors 2

David R. Plant (first)
Gordon S. Lynch (additional)

References 42 Referenced 36

10.1152/ajpcell.2000.279.5.C1564
10.1113/jphysiol.1988.sp017279
10.1152/ajpcell.1994.267.2.C507
10.1016/S0143-4160(96)90010-X
10.1111/j.1748-1716.1995.tb09886.x
10.1046/j.1365-201x.1998.00329.x
10.1007/BF00235039
10.1152/ajpcell.1986.251.3.C421
10.1016/0022-510X(92)90147-D
10.1093/gerona/50A.Special_Issue.124
10.1016/S0005-2736(97)00158-2
10.1074/jbc.273.10.5885
10.1152/ajpcell.2000.279.3.C611
10.1007/s002320010025
{'key': 'e_1_2_5_16_1', 'first-page': '26', 'article-title': 'MHC isoform expression and E‐C coupling in mechanically skinned single muscle fibres of the rat', 'volume': '43', 'author': 'Goodman C. A.', 'year': '2000', 'journal-title': 'The Physiologist'} / The Physiologist / MHC isoform expression and E‐C coupling in mechanically skinned single muscle fibres of the rat by Goodman C. A. (2000)
10.1113/jphysiol.1996.sp021402
10.1113/jphysiol.1990.sp018036
10.1113/jphysiol.1994.sp020253
10.1016/0022-510X(86)90143-7
10.1113/jphysiol.1989.sp017872
10.1152/ajpcell.1997.272.2.C638 / American Journal of Physiology / Effects of aging on shortening velocity and myosin isoform composition in single human skeletal muscle cells by Larsson L. (1997)
10.2165/00002512-200017040-00006
10.1152/ajpcell.1996.270.1.C352
10.1517/13543776.12.1.11
{'key': 'e_1_2_5_26_1', 'first-page': '147', 'article-title': 'The effects of age and low‐intensity endurance exercise on the contractile properties of single skinned fast‐ and slow‐twitch skeletal muscle fibers', 'volume': '57', 'author': 'Lynch G. S.', 'year': '1993', 'journal-title': 'Growth, Development & Aging'} / Growth, Development & Aging / The effects of age and low‐intensity endurance exercise on the contractile properties of single skinned fast‐ and slow‐twitch skeletal muscle fibers by Lynch G. S. (1993)
10.1111/j.1748-1716.1993.tb09528.x
10.1111/j.1469-7793.2001.00591.x
10.1046/j.1365-201x.1999.00624.x
10.1016/0304-4157(94)00014-5
10.1152/ajpcell.1996.271.4.C1032
10.1113/jphysiol.1997.sp021884
10.1113/jphysiol.1993.sp019589
10.1046/j.1440-1681.2001.03521.x
10.1113/jphysiol.2001.013369
10.1113/jphysiol.1996.sp021729
10.1111/j.1469-7793.2000.t01-2-00131.x
10.1007/s002329900233
10.1046/j.1365-201X.1998.0304f.x
10.1111/j.1748-1716.1992.tb09315.x
10.1152/jappl.1999.86.3.881
10.1016/S0006-3495(00)76742-7
{'key': 'e_1_2_5_43_1', 'first-page': '107', 'article-title': 'Peripheral nerve and muscle function in the aging Fischer 344/Brown Norway rat', 'volume': '59', 'author': 'Wineinger M. A.', 'year': '1995', 'journal-title': 'Growth Development & Aging'} / Growth Development & Aging / Peripheral nerve and muscle function in the aging Fischer 344/Brown Norway rat by Wineinger M. A. (1995)

Dates

Type	When
Created	22 years, 11 months ago (Oct. 1, 2002, 3:28 p.m.)
Deposited	2 years ago (Sept. 4, 2023, 9:27 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 3:57 p.m.)
Issued	23 years, 1 month ago (Aug. 1, 2002)
Published	23 years, 1 month ago (Aug. 1, 2002)
Published Online	23 years, 1 month ago (Aug. 1, 2002)
Published Print	23 years, 1 month ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Plant_2002, title={Excitation‐contraction coupling and sarcoplasmic reticulum function in lechanically skinned fibres from fast skeletal muscles of aged mice}, volume={543}, ISSN={1469-7793}, url={http://dx.doi.org/10.1113/jphysiol.2002.022418}, DOI={10.1113/jphysiol.2002.022418}, number={1}, journal={The Journal of Physiology}, publisher={Wiley}, author={Plant, David R. and Lynch, Gordon S.}, year={2002}, month=aug, pages={169–176} }

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