DOI: 10.1113/jphysiol.1976.sp011321. The preparation of human red cell ghosts containing calcium buffers.

The preparation of human red cell ghosts containing calcium buffers.

10.1113/jphysiol.1976.sp011321

Crossref journal-article

Wiley

The Journal of Physiology (311)

Abstract

1. Ca buffers may be introduced into human red cells by reversible haemolysis. The resealed ghosts retain Ca and chelating anions in the same ratio as in the haemolysing solution, enabling the intracellular Ca2+ concentration to be calculated simply. 2. The passive permeability of the ghosts to Na and Cl is unaffected by intracellular Ca2+ concentrations in the 10(‐8)‐10(‐4) M range, whereas the K permeability is greatly increased at concentrations above 10(‐7) M. 3. These preparations enable Ca‐dependent K movements to be studied under stable conditions. When the ghosts contain about 5 X 10(‐6) M‐Ca2+, over 96% of K transport occurs via the Ca‐sensitive route.

Bibliography

Simons, T. J. (1976). The preparation of human red cell ghosts containing calcium buffers. The Journal of Physiology, 256(1), 209â225. Portico.

Authors 1

T J Simons (first)

References 0 Referenced 41

None

Dates

Type	When
Created	10 years, 8 months ago (Dec. 19, 2014, 4:01 a.m.)
Deposited	1 year, 9 months ago (Nov. 6, 2023, 1:24 a.m.)
Indexed	1 year, 1 month ago (July 14, 2024, 6:11 a.m.)
Issued	49 years, 5 months ago (March 1, 1976)
Published	49 years, 5 months ago (March 1, 1976)
Published Online	49 years, 5 months ago (March 1, 1976)
Published Print	49 years, 5 months ago (March 1, 1976)

Funders 0

None

BibTeX

@article{Simons_1976, title={The preparation of human red cell ghosts containing calcium buffers.}, volume={256}, ISSN={1469-7793}, url={http://dx.doi.org/10.1113/jphysiol.1976.sp011321}, DOI={10.1113/jphysiol.1976.sp011321}, number={1}, journal={The Journal of Physiology}, publisher={Wiley}, author={Simons, T J}, year={1976}, month=mar, pages={209–225} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        14
      ]
    ],
    "date-time": "2024-07-14T10:11:16Z",
    "timestamp": 1720951876635
  },
  "reference-count": 0,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1976,
            3,
            1
          ]
        ],
        "date-time": "1976-03-01T00:00:00Z",
        "timestamp": 194486400000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1976,
        3
      ]
    ]
  },
  "abstract": "<jats:p>1. Ca buffers may be introduced into human red cells by reversible haemolysis. The resealed ghosts retain Ca and chelating anions in the same ratio as in the haemolysing solution, enabling the intracellular Ca2+ concentration to be calculated simply. 2. The passive permeability of the ghosts to Na and Cl is unaffected by intracellular Ca2+ concentrations in the 10(\u20108)\u201010(\u20104) M range, whereas the K permeability is greatly increased at concentrations above 10(\u20107) M. 3. These preparations enable Ca\u2010dependent K movements to be studied under stable conditions. When the ghosts contain about 5 X 10(\u20106) M\u2010Ca2+, over 96% of K transport occurs via the Ca\u2010sensitive route.</jats:p>",
  "DOI": "10.1113/jphysiol.1976.sp011321",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        12,
        19
      ]
    ],
    "date-time": "2014-12-19T09:01:43Z",
    "timestamp": 1418979703000
  },
  "page": "209-225",
  "source": "Crossref",
  "is-referenced-by-count": 41,
  "title": "The preparation of human red cell ghosts containing calcium buffers.",
  "prefix": "10.1113",
  "volume": "256",
  "author": [
    {
      "given": "T J",
      "family": "Simons",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        1976,
        3
      ]
    ]
  },
  "container-title": "The Journal of Physiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1113%2Fjphysiol.1976.sp011321",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://physoc.onlinelibrary.wiley.com/doi/pdf/10.1113/jphysiol.1976.sp011321",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        6
      ]
    ],
    "date-time": "2023-11-06T06:24:44Z",
    "timestamp": 1699251884000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://physoc.onlinelibrary.wiley.com/doi/10.1113/jphysiol.1976.sp011321"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1976,
        3
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1976,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1113/jphysiol.1976.sp011321"
  ],
  "URL": "http://dx.doi.org/10.1113/jphysiol.1976.sp011321",
  "relation": {},
  "ISSN": [
    "0022-3751",
    "1469-7793"
  ],
  "subject": [],
  "container-title-short": "The Journal of Physiology",
  "published": {
    "date-parts": [
      [
        1976,
        3
      ]
    ]
  }
}