DOI: 10.1111/mmi.12974. <scp>A</scp>fp14 is involved in regulating the length of Anti‐feeding prophage (<scp>A</scp>fp)

Afp14 is involved in regulating the length of Anti‐feeding prophage (Afp)

10.1111/mmi.12974

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe anti‐feeding prophage (Afp), a phage‐tail‐like particle that causes cessation of feeding in the New Zealand grass grub, Costelytra zealandica, is encoded by 18 open reading frames (afp1–18). C‐terminal truncations of afp14 resulted in shortened Afp particles, suggesting that Afp14 is involved in Afp length determination. We constructed an Afp assembly system (afp1–18), wherein Afp14 was truncated after the N‐terminal 88 residues. This construct, when expressed in trans in Escherichia coli expressing a N‐terminal 98‐amino acid Afp14 construct, yielded fully assembled Afp but no assembled Afp was detected in the case of a N‐terminal 96‐amino acid Afp14 construct. These results suggested that the 98 N‐terminal, amino acid residues of Afp14 is crucial for the initiation of Afp assembly via baseplate formation. Trans‐based expression of wild‐type afp14 resulted in Afp particles of varying lengths, all of which were shorter than the wild‐type Afp particle. On the other hand, similar expression of Afp14 harboring a C‐terminal extension (KLLEH6) resulted in elongated Afp particles. This information, combined with bioinformatics data, allowed us to propose a model delineating the mechanism and role of Afp14 in the maturation of the Afp particle.

Bibliography

Rybakova, D., Schramm, P., Mitra, A. K., & Hurst, M. R. H. (2015). Afp14 is involved in regulating the length of Antiâfeeding prophage (Afp). Molecular Microbiology, 96(4), 815â826. Portico.

Authors 4

Daria Rybakova (first)
Peter Schramm (additional)
Alok K. Mitra (additional)
Mark R. H. Hurst (additional)

References 31 Referenced 20

10.1006/viro.1994.1128
10.1111/j.1365-2958.2005.04758.x
10.1093/nar/25.17.3389
{'issue': '9', 'key': 'e_1_2_6_5_1', 'first-page': 'S10', 'article-title': 'The evolution of the tape measure protein: units, duplications and losses BMC', 'volume': '12', 'author': 'Belcaid M.', 'year': '2011', 'journal-title': 'Bioinformatics'} / Bioinformatics / The evolution of the tape measure protein: units, duplications and losses BMC by Belcaid M. (2011)
10.1016/j.mib.2006.01.002
10.1074/jbc.M113.456145
10.1128/jb.176.17.5439-5449.1994
10.1128/JB.186.15.5116-5128.2004
10.1111/j.1574-6968.2007.00645.x
10.1016/S0022-2836(65)80107-3
10.1126/science.1091422
10.1038/327073a0
10.1016/0065-227X(90)90004-D
10.1016/0092-8674(84)90476-8
10.1016/j.jtbi.2009.12.015
10.1038/nprot.2009.2
{'key': 'e_1_2_6_18_1', 'first-page': '19', 'article-title': 'Max efficiency DH10B™: a new host for cloning methylated DNA', 'volume': '12', 'author': 'Lorow D.', 'year': '1990', 'journal-title': 'Focus'} / Focus / Max efficiency DH10B™: a new host for cloning methylated DNA by Lorow D. (1990)
10.1093/nar/gkq1189
10.1128/JB.01215-12
10.1046/j.1365-2958.2000.02135.x
10.1006/viro.2000.0497
10.1016/S0092-8674(03)00233-2
10.4161/viru.1.6.13800
10.1111/j.1462-2920.2008.01861.x
10.1111/mmi.12305
{'key': 'e_1_2_6_27_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual by Sambrook J. (1989)
10.1093/gbe/evu136
10.1128/JB.01363-08
10.1016/0022-2836(86)90385-2
10.1128/JB.182.3.680-688.2000
10.1111/j.1365-2958.2008.06556.x

Dates

Type	When
Created	10 years, 6 months ago (Feb. 17, 2015, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:27 a.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:37 p.m.)
Issued	10 years, 5 months ago (March 16, 2015)
Published	10 years, 5 months ago (March 16, 2015)
Published Online	10 years, 5 months ago (March 16, 2015)
Published Print	10 years, 3 months ago (May 1, 2015)

Funders 0

None

BibTeX

@article{Rybakova_2015, title={<scp>A</scp>fp14 is involved in regulating the length of Anti‐feeding prophage (<scp>A</scp>fp)}, volume={96}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/mmi.12974}, DOI={10.1111/mmi.12974}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Rybakova, Daria and Schramm, Peter and Mitra, Alok K. and Hurst, Mark R. H.}, year={2015}, month=mar, pages={815–826} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:37:11Z",
    "timestamp": 1752550631079
  },
  "reference-count": 31,
  "publisher": "Wiley",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2015,
            3,
            16
          ]
        ],
        "date-time": "2015-03-16T00:00:00Z",
        "timestamp": 1426464000000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2015,
        5
      ]
    ]
  },
  "abstract": "<jats:title>Summary</jats:title><jats:p>The anti\u2010feeding prophage (<jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp), a phage\u2010tail\u2010like particle that causes cessation of feeding in the <jats:styled-content style=\"fixed-case\">N</jats:styled-content>ew <jats:styled-content style=\"fixed-case\">Z</jats:styled-content>ealand grass grub, <jats:styled-content style=\"fixed-case\"><jats:italic>C</jats:italic></jats:styled-content><jats:italic>ostelytra zealandica</jats:italic>, is encoded by 18 open reading frames (<jats:styled-content style=\"fixed-case\"><jats:italic>afp1\u201318</jats:italic></jats:styled-content>). <jats:styled-content style=\"fixed-case\">C</jats:styled-content>\u2010terminal truncations of <jats:styled-content style=\"fixed-case\"><jats:italic>afp14</jats:italic></jats:styled-content> resulted in shortened <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp particles, suggesting that <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 is involved in <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp length determination. We constructed an <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp assembly system (<jats:styled-content style=\"fixed-case\"><jats:italic>afp1\u201318</jats:italic></jats:styled-content>), wherein <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 was truncated after the <jats:styled-content style=\"fixed-case\">N</jats:styled-content>\u2010terminal 88 residues. This construct, when expressed <jats:italic>in trans</jats:italic> in <jats:styled-content style=\"fixed-case\"><jats:italic>E</jats:italic></jats:styled-content><jats:italic>scherichia coli</jats:italic> expressing a <jats:styled-content style=\"fixed-case\">N</jats:styled-content>\u2010terminal 98\u2010amino acid <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 construct, yielded fully assembled <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp but no assembled <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp was detected in the case of a <jats:styled-content style=\"fixed-case\">N</jats:styled-content>\u2010terminal 96\u2010amino acid <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 construct. These results suggested that the 98 <jats:styled-content style=\"fixed-case\">N</jats:styled-content>\u2010terminal, amino acid residues of <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 is crucial for the initiation of <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp assembly <jats:italic>via</jats:italic> baseplate formation. <jats:italic>Trans</jats:italic>\u2010based expression of wild\u2010type <jats:styled-content style=\"fixed-case\"><jats:italic>afp14</jats:italic></jats:styled-content> resulted in <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp particles of varying lengths, all of which were shorter than the wild\u2010type <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp particle. On the other hand, similar expression of <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 harboring a <jats:styled-content style=\"fixed-case\">C</jats:styled-content>\u2010terminal extension (<jats:styled-content style=\"fixed-case\">KLLEH</jats:styled-content><jats:sub>6</jats:sub>) resulted in elongated <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp particles. This information, combined with bioinformatics data, allowed us to propose a model delineating the mechanism and role of <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp14 in the maturation of the <jats:styled-content style=\"fixed-case\">A</jats:styled-content>fp particle.</jats:p>",
  "DOI": "10.1111/mmi.12974",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        2,
        17
      ]
    ],
    "date-time": "2015-02-17T08:45:08Z",
    "timestamp": 1424162708000
  },
  "page": "815-826",
  "source": "Crossref",
  "is-referenced-by-count": 20,
  "title": "<scp>A</scp>fp14 is involved in regulating the length of Anti\u2010feeding prophage (<scp>A</scp>fp)",
  "prefix": "10.1111",
  "volume": "96",
  "author": [
    {
      "given": "Daria",
      "family": "Rybakova",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Innovative Farm Systems AgResearch Lincoln Research Centre  Private Bag 4749 Christchurch 8140 New Zealand"
        }
      ]
    },
    {
      "given": "Peter",
      "family": "Schramm",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Academy of Life Science, Engineering, and Design Saxion University of Applied Science  Enschede M.H. Tromplaan 28 7513 AB Enschede The Netherlands"
        }
      ]
    },
    {
      "given": "Alok K.",
      "family": "Mitra",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "School of Biological Sciences University of Auckland  Thomas Building 3A Symonds Street Auckland New Zealand"
        }
      ]
    },
    {
      "given": "Mark R. H.",
      "family": "Hurst",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Innovative Farm Systems AgResearch Lincoln Research Centre  Private Bag 4749 Christchurch 8140 New Zealand"
        }
      ]
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2015,
        3,
        16
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.1994.1128"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2005.04758.x"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/25.17.3389"
    },
    {
      "issue": "9",
      "key": "e_1_2_6_5_1",
      "first-page": "S10",
      "article-title": "The evolution of the tape measure protein: units, duplications and losses BMC",
      "volume": "12",
      "author": "Belcaid M.",
      "year": "2011",
      "journal-title": "Bioinformatics"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.mib.2006.01.002"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M113.456145"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.176.17.5439-5449.1994"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.186.15.5116-5128.2004"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1574-6968.2007.00645.x"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2836(65)80107-3"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1091422"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/327073a0"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0065-227X(90)90004-D"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90476-8"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jtbi.2009.12.015"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nprot.2009.2"
    },
    {
      "key": "e_1_2_6_18_1",
      "first-page": "19",
      "article-title": "Max efficiency DH10B\u2122: a new host for cloning methylated DNA",
      "volume": "12",
      "author": "Lorow D.",
      "year": "1990",
      "journal-title": "Focus"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/gkq1189"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.01215-12"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2000.02135.x"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.2000.0497"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(03)00233-2"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.4161/viru.1.6.13800"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1462-2920.2008.01861.x"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/mmi.12305"
    },
    {
      "key": "e_1_2_6_27_1",
      "volume-title": "Molecular Cloning: A Laboratory Manual",
      "author": "Sambrook J.",
      "year": "1989"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/gbe/evu136"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.01363-08"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(86)90385-2"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.182.3.680-688.2000"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2008.06556.x"
    }
  ],
  "container-title": "Molecular Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmmi.12974",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.12974",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T08:27:22Z",
    "timestamp": 1694852842000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/mmi.12974"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2015,
        3,
        16
      ]
    ]
  },
  "references-count": 31,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2015,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/mmi.12974"
  ],
  "URL": "http://dx.doi.org/10.1111/mmi.12974",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2015,
        3,
        16
      ]
    ]
  }
}