DOI: 10.1111/j.1751-1097.1995.tb05248.x. CATALASE INACTIVATION FOLLOWING PHOTOSENSITIZATION WITH TETRASULFONATEDMETALLOPHTHALOCYANINES

CATALASE INACTIVATION FOLLOWING PHOTOSENSITIZATION WITH TETRASULFONATEDMETALLOPHTHALOCYANINES

10.1111/j.1751-1097.1995.tb05248.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— Catalase (CAT) in solution or incorporated in erythrocytes and K562 leukemic cells is inactivated during photosensitization with tetrasulfonated metallophthalocyantnes (MePcS4). The effect of added scavengers and D20 showed that both singlet oxygen and free radical species are involved in this process. Evidence was found that direct interactions of ground or excited‐stated photosensitizers with CAT are not responsible for CAT inactivation. Specific techniques to probe early damage to the CAT structure involved optical and EPR spectroscopy, HPLC and polyacrylamide gel electrophoresis analyses. Different primary events of photosensitized protein damage included oxidation of cysteine residues as well as other amino acids, as demonstrated by the formation of carbon‐centered free radicals and the loss of absorbance at λ= 275 nm. In parallel, we detected degradation of the CAT heme groups, accompanied by release of Fe(II) ions in solution. These combined phenomena initiate cross‐linkages between CAT subunits and subsequent degradation of the protein with formation of irreversible aggregates in solution. Phthalocyanine‐mediated photoinactivation of cell‐bound CAT results in loss of protection against accumulating H202, providing an additional pathway of phototoxicity.

Bibliography

Gantchev, T. G., & van Lier, J. E. (1995). CATALASE INACTIVATION FOLLOWING PHOTOSENSITIZATION WITH TETRASULFONATEDMETALLOPHTHALOCYANINES. Photochemistry and Photobiology, 62(1), 123â134. Portico.

Authors 2

Tsvetan G. Gantchev (first)
Johan E. van Lier (additional)

References 34 Referenced 39

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10.1111/j.1432-1033.1967.tb00088.x
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10.1152/physrev.1979.59.3.527
{'key': 'e_1_2_2_6_2', 'first-page': '273', 'volume-title': 'Methods of Enzymatic Analysis', 'author': 'Aebi H. E.', 'year': '1984'} / Methods of Enzymatic Analysis by Aebi H. E. (1984)
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10.1111/j.1751-1097.1986.tb03574.x
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10.1080/09553009114552431
10.1111/j.1751-1097.1988.tb02761.x
{'key': 'e_1_2_2_14_2', 'first-page': '17', 'volume-title': 'Photosensitizing Compounds: Their Chemistry, Biology and Chemical Use', 'author': 'Lier J. E.', 'year': '1989'} / Photosensitizing Compounds: Their Chemistry, Biology and Chemical Use by Lier J. E. (1989)
{'key': 'e_1_2_2_15_2', 'first-page': '101', 'article-title': 'Spin‐trapping of free radicals during phthalocyanine photosensitization of lymphoma cell in vitro', 'volume': '13', 'author': 'Gantchev T. G.', 'year': '1992', 'journal-title': 'Cancer Biochem. Biophys.'} / Cancer Biochem. Biophys. / Spin‐trapping of free radicals during phthalocyanine photosensitization of lymphoma cell in vitro by Gantchev T. G. (1992)
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{'key': 'e_1_2_2_24_2', 'first-page': '705', 'article-title': 'DNA strand scission and base release photosensitized by metallo‐phthalocyanines', 'volume': '66', 'author': 'Gantchev T. G.', 'year': '1994', 'journal-title': 'Int. J. Radiat. Biol.'} / Int. J. Radiat. Biol. / DNA strand scission and base release photosensitized by metallo‐phthalocyanines by Gantchev T. G. (1994)
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{'key': 'e_1_2_2_27_2', 'first-page': '21', 'volume-title': 'Detection of active oxygen species in a photosensitizing reaction in vitro', 'author': 'Miyoshi N.', 'year': '1994'} / Detection of active oxygen species in a photosensitizing reaction in vitro by Miyoshi N. (1994)
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{'key': 'e_1_2_2_31_2', 'first-page': '295', 'article-title': 'Thiyl free radical production with he‐matoporphyrin derivative, cysteine and light: a spin‐trapping study', 'volume': '111', 'author': 'Buettner G. R.', 'year': '1985', 'journal-title': 'FEBS Lett.'} / FEBS Lett. / Thiyl free radical production with he‐matoporphyrin derivative, cysteine and light: a spin‐trapping study by Buettner G. R. (1985)
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Dates

Type	When
Created	17 years, 7 months ago (Jan. 2, 2008, 2:38 p.m.)
Deposited	1 year, 9 months ago (Oct. 26, 2023, 1:32 a.m.)
Indexed	1 year ago (Aug. 6, 2024, 2 a.m.)
Issued	30 years, 1 month ago (July 1, 1995)
Published	30 years, 1 month ago (July 1, 1995)
Published Online	17 years, 7 months ago (Jan. 2, 2008)
Published Print	30 years, 1 month ago (July 1, 1995)

Funders 0

None

BibTeX

@article{Gantchev_1995, title={CATALASE INACTIVATION FOLLOWING PHOTOSENSITIZATION WITH TETRASULFONATEDMETALLOPHTHALOCYANINES}, volume={62}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1995.tb05248.x}, DOI={10.1111/j.1751-1097.1995.tb05248.x}, number={1}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Gantchev, Tsvetan G. and van Lier, Johan E.}, year={1995}, month=jul, pages={123–134} }

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