DOI: 10.1111/j.1751-1097.1993.tb09218.x. EFFECT OF UVA AND BLUE LIGHT ON PORPHYRIN BIOSYNTHESIS IN EPIDERMAL CELLS*

EFFECT OF UVA AND BLUE LIGHT ON PORPHYRIN BIOSYNTHESIS IN EPIDERMAL CELLS*

10.1111/j.1751-1097.1993.tb09218.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— To study porphyrin biosynthesis in normal human keratinocytes and A431 cells derived from human epidermoid carcinoma, cultured cells were incubated with delta‐aminolevulinic acid (ALA), the precursor of porphyrin synthesis, and accumulation of porphyrins was measured spectrofluorometrically. Both human keratinocytes and A431 cells accumulated porphyrins in a time‐dependent and a dose‐dependent fashion. Protoporphyrin was the predominant porphyrin accumulated by both cell types. Porphyrin accumulation was enhanced by Ca Mg ethylene‐diaminetetraacetic acid, a ferrochelatase inhibitor, and the enhancement was reversed by the addition of iron, suggesting the utilization of iron by ferrochelatase. The effect of light on porphyrin accumulation was evaluated by exposing the ALA‐loaded A431 cells to ultraviolet‐A (UVA) and blue light radiation, followed by continued incubation with ALA for 2–48 h. There was an enhancement of porphyrin accumulation 2–48 h after the radiation as compared with nonirradiated controls. Consistent with this finding, ferrochelatase activity decreased in these cells at 24 h and 48 h. These data demonstrate that human keratinocytes and A431 cells are capable of porphyrin biosynthesis, and that exposure of porphyrin‐containing A431 cells to light, which includes the Soret band spectrum, decreases the ferrochelatase activity, which is responsible, at least in part, for the further increase in porphyrin level.

Bibliography

Sassa, D. H. S., & Lim, H. W. (1993). EFFECT OF UVA AND BLUE LIGHT ON PORPHYRIN BIOSYNTHESIS IN EPIDERMAL CELLS*. Photochemistry and Photobiology, 57(5), 825â829. Portico.

Authors 2

Dan He Shigeru Sassa (first)
Henry W. Lim (additional)

References 17 Referenced 37

10.1111/j.1751-1097.1986.tb04726.x
10.1016/0304-3835(87)90096-6
10.1111/1523-1747.ep12485121
He D. E.Karas S.SassaandH. W.Lim(1992)Porphyrin synthesis by murine epidermal cells.Skin Pharmacol.(In press). (10.1159/000211080)
10.1007/978-1-4684-8312-3_6
10.1084/jem.153.5.1094
10.1016/S0021-9258(19)52451-6
10.1111/j.1751-1097.1989.tb04133.x
10.1159/000459104 / Enzyme / A radiochemical assay for heme synthase activity by Bloomer J. R. (1982)
10.1016/0167-4838(90)90032-B
10.1016/S0021-9258(19)40633-9 / J. Biol. Chem. / Effects by heme, insulin, and serum albumin on heme and protein synthesis in chick embryo liver cells cultured in a chemically denned medium, and a spectrofluorometric assay for porphyrin composition by Granick S. (1975)
10.1016/S0021-9258(17)40571-0 / J. Biol. Chem. / Induction of aminolevulinate synthase and porphyrins in cultured liver cells maintained in chemically denned medium. Permissive effects of hormones on induction process by Sassa S. (1977)
{'key': 'e_1_2_1_14_2', 'first-page': '2394', 'volume-title': 'Internal Medicine', 'author': 'Elin R. J.', 'year': '1988'} / Internal Medicine by Elin R. J. (1988)
{'key': 'e_1_2_1_15_2', 'first-page': '1666', 'volume-title': 'Dermatology in General Medicine', 'author': 'Bickers D. R.', 'year': '1987'} / Dermatology in General Medicine by Bickers D. R. (1987)
10.1172/JCI110520
{'key': 'e_1_2_1_17_2', 'first-page': '14', 'volume-title': 'Erythropoietic Protoporphyria', 'author': 'Baart De La Faille H.', 'year': '1975'} / Erythropoietic Protoporphyria by Baart De La Faille H. (1975)
10.1172/JCI108895

Dates

Type	When
Created	17 years, 7 months ago (Jan. 2, 2008, 3:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 1:12 a.m.)
Indexed	1 year, 9 months ago (Oct. 28, 2023, 8:55 p.m.)
Issued	32 years, 3 months ago (May 1, 1993)
Published	32 years, 3 months ago (May 1, 1993)
Published Online	17 years, 7 months ago (Jan. 2, 2008)
Published Print	32 years, 3 months ago (May 1, 1993)

Funders 0

None

BibTeX

@article{Sassa_1993, title={EFFECT OF UVA AND BLUE LIGHT ON PORPHYRIN BIOSYNTHESIS IN EPIDERMAL CELLS*}, volume={57}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1993.tb09218.x}, DOI={10.1111/j.1751-1097.1993.tb09218.x}, number={5}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Sassa, Dan He Shigeru and Lim, Henry W.}, year={1993}, month=may, pages={825–829} }

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