DOI: 10.1111/j.1751-1097.1987.tb08413.x. LIGHT‐ABSORPTION AND ELECTRON‐TRANSPORT BALANCE BETWEEN PHOTOSYSTEM II AND PHOTOSYSTEM I IN SPINACH CHLOROPLASTS

LIGHT‐ABSORPTION AND ELECTRON‐TRANSPORT BALANCE BETWEEN PHOTOSYSTEM II AND PHOTOSYSTEM I IN SPINACH CHLOROPLASTS

10.1111/j.1751-1097.1987.tb08413.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract—The distribution of absorbed light and the turnover of electrons by the two photosystems in spinach chloroplasts was investigated. This was implemented upon quantitation of photochemical reaction centers, chlorophyll antenna size and composition of each photosystem (PS), and rate of light absorptionin situ.In spinach chloroplasts, the photosystem stoichiometry was PSIIJPSIIα/PSIIβ/PSI= 1.3/0.4/1.0. The number (N) of chlorophyll (a+b) molecules associated with each PS was N(PSIIα)/N(PSIIβ)/N(PSI)=230/100/200, i.e. about 65% of all Chl is associated with PSII and about 35% with PSI. Light absorption by PSIIin vivois selectively attenuated at the molecular, membrane and leaf levels, (a) The rate of light absorption by PSII was only 0.85 that of PSI because of the lower rate of light absorption by Chlbas compared to Chla(approximately 80% of all Chlbin the chloroplast is associated with PSII). (b) The exclusive localization of PSIIαin the membrane of the grana partition regions and of PSI in intergrana lamellae resulted in a differential “sieve effect” or “flattening of absorbance” by the photosystems in the two membrane regions. Due to this phenomenon, the rate of light absorption by PSII was lower than that of PSI by 15‐20%. (c) Selective filtering of sunlight through the spinach leaf results in a substantial distortion of the effective absorbance spectra and concomitant attenuation of light absorption by the two photosystems. Such attenuation was greater for PSII than for PSI because the latter benefits from light absorption in the 700‐730 nm region.It is concluded that, in spite of its stoichiometric excess in spinach chloroplasts, light absorption by PSII is not greater than that by PSI due to the different molecular composition of the two light‐harvesting antenna systems, due to the localization of PSII in the grana, and also because of the light transmission properties through the leaf. The elevated PSII/PSI reaction center ratio of 1.7 and the association of 65% of all Chl with PSII help to counter the multilevel attenuation of light absorption by PSII and ensure a balanced PSII/PSI electron turnover ratio of about 1:1.

Bibliography

MELIS, A., Spangfort, M., & Andersson, B. (1987). LIGHTâABSORPTION AND ELECTRONâTRANSPORT BALANCE BETWEEN PHOTOSYSTEM II AND PHOTOSYSTEM I IN SPINACH CHLOROPLASTS. Photochemistry and Photobiology, 45(1), 129â136. Portico.

Authors 3

Anastasios MELIS (first)
Michael Spangfort (additional)
Bertil Andersson (additional)

References 37 Referenced 131

10.1104/pp.79.3.872
10.1016/0014-5793(80)80973-2
10.1111/j.1751-1097.1983.tb03863.x
10.1073/pnas.80.3.745
10.1016/0005-2728(80)90078-X
10.1016/0014-5793(82)80695-9
10.1104/pp.24.1.1
10.1104/pp.52.3.257
10.1016/0014-5793(81)80608-4
{'key': 'e_1_2_1_11_1', 'first-page': '85', 'article-title': 'Characterization of the light climate on the floor of a queensland rainforest', 'volume': '71', 'author': 'Björkman O.', 'year': '1972', 'journal-title': 'Carnegie Yearbook'} / Carnegie Yearbook / Characterization of the light climate on the floor of a queensland rainforest by Björkman O. (1972)
10.1016/0003-9861(78)90031-0
10.1073/pnas.74.8.3377
10.1002/j.1537-2197.1943.tb14744.x
10.1104/pp.74.4.993
10.1016/0005-2728(69)90068-1
10.1016/0005-2728(83)90036-1
10.1016/0014-5793(85)80930-3
10.1104/pp.41.3.533
10.1016/S0165-8646(24)00319-2 / Photoblochem. Photobiophys. / The influence of membrane stacking on light absorption by chloroplasts by Jennings R. C. (1985)
10.1016/0005-2728(83)90139-1
10.1016/0005-2728(82)90320-6
10.1016/S0021-9258(18)73399-1 / J. Biol. Chem. / Criteria for purity of chlorophyll preparations by MacKinney G. (1940)
10.1016/0005-2728(86)90023-X
10.1002/jcb.240240308
10.1016/0005-2728(85)90017-9
10.1016/0005-2728(83)90108-1
10.1073/pnas.77.8.4712
10.1016/0005-2728(81)90219-X
{'key': 'e_1_2_1_30_1', 'first-page': '757', 'article-title': 'The mechanism of photosynthetic membrane adaptation to environmental stress conditions: a hypothesis on the role of electron‐transport capacity and of ATP/NADPH pool in the regulation of thylakdid membrane organization and function', 'volume': '23', 'author': 'Melis A.', 'year': '1985', 'journal-title': 'Physiol. Veg.'} / Physiol. Veg. / The mechanism of photosynthetic membrane adaptation to environmental stress conditions: a hypothesis on the role of electron‐transport capacity and of ATP/NADPH pool in the regulation of thylakdid membrane organization and function by Melis A. (1985)
10.1104/pp.65.5.814
10.1111/j.1399-3054.1969.tb07406.x
10.1111/j.1399-3054.1980.tb02685.x
10.1111/j.1432-1033.1980.tb06035.x / Eur. J. Biochem. / The role of the light‐harvesting chlorophyll al b‐protein complex in chloroplast membrane stacking by Ryrie I. J. (1980)
10.1016/0005-2728(67)90015-1
10.1093/oxfordjournals.pcp.a076971
10.1016/0003-9861(84)90401-6
10.1086/334981

Dates

Type	When
Created	17 years, 8 months ago (Jan. 2, 2008, 4:27 p.m.)
Deposited	7 months, 1 week ago (Jan. 25, 2025, midnight)
Indexed	1 week ago (Aug. 28, 2025, 8:09 a.m.)
Issued	38 years, 8 months ago (Jan. 1, 1987)
Published	38 years, 8 months ago (Jan. 1, 1987)
Published Online	17 years, 8 months ago (Jan. 2, 2008)
Published Print	38 years, 8 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{MELIS_1987, title={LIGHT‐ABSORPTION AND ELECTRON‐TRANSPORT BALANCE BETWEEN PHOTOSYSTEM II AND PHOTOSYSTEM I IN SPINACH CHLOROPLASTS}, volume={45}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1987.tb08413.x}, DOI={10.1111/j.1751-1097.1987.tb08413.x}, number={1}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={MELIS, Anastasios and Spangfort, Michael and Andersson, Bertil}, year={1987}, month=jan, pages={129–136} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        28
      ]
    ],
    "date-time": "2025-08-28T12:09:47Z",
    "timestamp": 1756382987492,
    "version": "3.33.0"
  },
  "reference-count": 37,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            1,
            2
          ]
        ],
        "date-time": "2008-01-02T00:00:00Z",
        "timestamp": 1199232000000
      },
      "content-version": "vor",
      "delay-in-days": 7671,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        1
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>Abstract\u2014</jats:bold>The distribution of absorbed light and the turnover of electrons by the two photosystems in spinach chloroplasts was investigated. This was implemented upon quantitation of photochemical reaction centers, chlorophyll antenna size and composition of each photosystem (PS), and rate of light absorption<jats:italic>in situ.</jats:italic>In spinach chloroplasts, the photosystem stoichiometry was PSIIJPSII<jats:sub>\u03b1</jats:sub>/PSII<jats:sub>\u03b2</jats:sub>/PSI= 1.3/0.4/1.0. The number (N) of chlorophyll (<jats:italic>a+b</jats:italic>) molecules associated with each PS was N(PSII<jats:sub>\u03b1</jats:sub>)/N(PSII<jats:sub>\u03b2</jats:sub>)/N(PSI)=230/100/200, i.e. about 65% of all Chl is associated with PSII and about 35% with PSI. Light absorption by PSII<jats:italic>in vivo</jats:italic>is selectively attenuated at the molecular, membrane and leaf levels, (a) The rate of light absorption by PSII was only 0.85 that of PSI because of the lower rate of light absorption by Chl<jats:italic>b</jats:italic>as compared to Chl<jats:italic>a</jats:italic>(approximately 80% of all Chl<jats:italic>b</jats:italic>in the chloroplast is associated with PSII). (b) The exclusive localization of PSII<jats:sub>\u03b1</jats:sub>in the membrane of the grana partition regions and of PSI in intergrana lamellae resulted in a differential \u201csieve effect\u201d or \u201cflattening of absorbance\u201d by the photosystems in the two membrane regions. Due to this phenomenon, the rate of light absorption by PSII was lower than that of PSI by 15\u201020%. (c) Selective filtering of sunlight through the spinach leaf results in a substantial distortion of the effective absorbance spectra and concomitant attenuation of light absorption by the two photosystems. Such attenuation was greater for PSII than for PSI because the latter benefits from light absorption in the 700\u2010730 nm region.</jats:p><jats:p>It is concluded that, in spite of its stoichiometric excess in spinach chloroplasts, light absorption by PSII is not greater than that by PSI due to the different molecular composition of the two light\u2010harvesting antenna systems, due to the localization of PSII in the grana, and also because of the light transmission properties through the leaf. The elevated PSII/PSI reaction center ratio of 1.7 and the association of 65% of all Chl with PSII help to counter the multilevel attenuation of light absorption by PSII and ensure a balanced PSII/PSI electron turnover ratio of about 1:1.</jats:p>",
  "DOI": "10.1111/j.1751-1097.1987.tb08413.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        1,
        2
      ]
    ],
    "date-time": "2008-01-02T21:27:02Z",
    "timestamp": 1199309222000
  },
  "page": "129-136",
  "source": "Crossref",
  "is-referenced-by-count": 131,
  "title": "LIGHT\u2010ABSORPTION AND ELECTRON\u2010TRANSPORT BALANCE BETWEEN PHOTOSYSTEM II AND PHOTOSYSTEM I IN SPINACH CHLOROPLASTS",
  "prefix": "10.1111",
  "volume": "45",
  "author": [
    {
      "given": "Anastasios",
      "family": "MELIS",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Michael",
      "family": "Spangfort",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Bertil",
      "family": "Andersson",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        1,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.79.3.872"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(80)80973-2"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1751-1097.1983.tb03863.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.80.3.745"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(80)90078-X"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(82)80695-9"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.24.1.1"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.52.3.257"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(81)80608-4"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "85",
      "article-title": "Characterization of the light climate on the floor of a queensland rainforest",
      "volume": "71",
      "author": "Bj\u00f6rkman O.",
      "year": "1972",
      "journal-title": "Carnegie Yearbook"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(78)90031-0"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.74.8.3377"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1537-2197.1943.tb14744.x"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.74.4.993"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(69)90068-1"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(83)90036-1"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(85)80930-3"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.41.3.533"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "215",
      "DOI": "10.1016/S0165-8646(24)00319-2",
      "article-title": "The influence of membrane stacking on light absorption by chloroplasts",
      "volume": "9",
      "author": "Jennings R. C.",
      "year": "1985",
      "journal-title": "Photoblochem. Photobiophys."
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(83)90139-1"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(82)90320-6"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "crossref",
      "first-page": "91",
      "DOI": "10.1016/S0021-9258(18)73399-1",
      "article-title": "Criteria for purity of chlorophyll preparations",
      "volume": "132",
      "author": "MacKinney G.",
      "year": "1940",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(86)90023-X"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcb.240240308"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(85)90017-9"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(83)90108-1"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.8.4712"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(81)90219-X"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "757",
      "article-title": "The mechanism of photosynthetic membrane adaptation to environmental stress conditions: a hypothesis on the role of electron\u2010transport capacity and of ATP/NADPH pool in the regulation of thylakdid membrane organization and function",
      "volume": "23",
      "author": "Melis A.",
      "year": "1985",
      "journal-title": "Physiol. Veg."
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.65.5.814"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3054.1969.tb07406.x"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3054.1980.tb02685.x"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "crossref",
      "first-page": "345",
      "DOI": "10.1111/j.1432-1033.1980.tb06035.x",
      "article-title": "The role of the light\u2010harvesting chlorophyll al b\u2010protein complex in chloroplast membrane stacking",
      "volume": "107",
      "author": "Ryrie I. J.",
      "year": "1980",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(67)90015-1"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.pcp.a076971"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(84)90401-6"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/334981"
    }
  ],
  "container-title": "Photochemistry and Photobiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1751-1097.1987.tb08413.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1751-1097.1987.tb08413.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        1,
        25
      ]
    ],
    "date-time": "2025-01-25T05:00:21Z",
    "timestamp": 1737781221000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.1987.tb08413.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        1
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1987,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1751-1097.1987.tb08413.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1751-1097.1987.tb08413.x",
  "relation": {},
  "ISSN": [
    "0031-8655",
    "1751-1097"
  ],
  "subject": [],
  "container-title-short": "Photochem &amp; Photobiology",
  "published": {
    "date-parts": [
      [
        1987,
        1
      ]
    ]
  }
}