DOI: 10.1111/j.1751-1097.1987.tb07896.x. PURIFICATION OF PHYTOCHROME FROM RYE BY FAST PROTEIN LIQUID CHROMATOGRAPHY

PURIFICATION OF PHYTOCHROME FROM RYE BY FAST PROTEIN LIQUID CHROMATOGRAPHY

10.1111/j.1751-1097.1987.tb07896.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract. A rapid procedure for the purification of phytochrome from rye by fast protein liquid chromatography (FPLC) is described. A pre‐purification with Q‐Sepharose fast flow and hydroxylapatite fast flow is necessary in order not to exceed the total capacity of the Mono‐Q HR 5/5 column. The whole fractionation is finished within 24 h and yields an intact 124‐kDa phytochrome of high purity.

Bibliography

Ernst, D., Vojacek, R., & Oesterhelt, D. (1987). PURIFICATION OF PHYTOCHROME FROM RYE BY FAST PROTEIN LIQUID CHROMATOGRAPHY. Photochemistry and Photobiology, 45(S1), 859â862. Portico.

Authors 3

D. Ernst (first)
R. Vojacek (additional)
D. Oesterhelt (additional)

References 14 Referenced 3

10.1016/0003-2697(76)90527-3
10.1111/j.1751-1097.1985.tb03478.x
10.1016/0003-2697(85)90206-4
10.1002/j.1460-2075.1984.tb02261.x / EMBO J. / Purified phytochrome influences in vitro transcription in rye nuclei by Ernst D. (1984)
Górg A. W.Postel J.Weser W. H.BoeskenandH. W.Schiwara(1985)Horizontale SDS‐Elektrophorese in ultradünnen Gradientengelen zur Differenzierung von Urinproben. LKB Produkter Germany Application Note.
10.1016/0304-4211(83)90108-6
10.1016/0014-5793(82)80729-1
10.1038/227680a0
{'key': 'e_1_2_2_10_1', 'first-page': '11 025', 'article-title': 'Structure‐function studies on phytochrome', 'volume': '258', 'author': 'Litts J. C.', 'year': '1983', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. / Structure‐function studies on phytochrome by Litts J. C. (1983)
10.1007/978-3-642-96453-4_27
10.1007/978-1-4684-3551-1_2
10.1016/0003-2697(79)90732-2
10.1073/pnas.79.17.5272
10.1021/bi00279a029

Dates

Type	When
Created	17 years, 7 months ago (Jan. 2, 2008, 4:21 p.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 5:18 a.m.)
Indexed	1 year, 11 months ago (Sept. 28, 2023, 1:42 a.m.)
Issued	38 years, 3 months ago (May 1, 1987)
Published	38 years, 3 months ago (May 1, 1987)
Published Online	17 years, 7 months ago (Jan. 2, 2008)
Published Print	38 years, 3 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Ernst_1987, title={PURIFICATION OF PHYTOCHROME FROM RYE BY FAST PROTEIN LIQUID CHROMATOGRAPHY}, volume={45}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1987.tb07896.x}, DOI={10.1111/j.1751-1097.1987.tb07896.x}, number={S1}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Ernst, D. and Vojacek, R. and Oesterhelt, D.}, year={1987}, month=may, pages={859–862} }

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